Basic Study
Copyright ©The Author(s) 2019. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Clin Cases. Jun 26, 2019; 7(12): 1383-1392
Published online Jun 26, 2019. doi: 10.12998/wjcc.v7.i12.1383
Study on gene expression patterns and functional pathways of peripheral blood monocytes reveals potential molecular mechanism of surgical treatment for periodontitis
Jin-Ji Ma, Hong-Mei Liu, Xiang-Hua Xu, Li-Xin Guo, Qing Lin
Jin-Ji Ma, Jinan Stomatological Hospital Gaoxin Branch, Jinan 250001, Shandong Province, China
Hong-Mei Liu, Li-Xin Guo, Qing Lin, Department of Endodontics, Jinan Stomatological Hospital, Jinan 250001, Shandong Province, China
Xiang-Hua Xu, Department of Stomatology, Shandong Provincial Hospital, Jinan 250001, Shandong Province, China
Author contributions: Ma JJ and Liu HM contributed equally to this work. Ma JJ, Liu HM, and Xu XH designed the research; Ma JJ, Liu HM, and Guo LX performed the research; Xu XH and Lin Q analyzed the data; Ma JJ, Liu HM, and Lin Q wrote the paper.
Institutional review board statement: This study was reviewed and approved by the Institutional Review Board of Jinan Stomatological Hospital.
Conflict-of-interest statement: The authors declare no conflict of interest.
Data sharing statement: No additional data are available.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See:
Corresponding author: Qing Lin, BSc, Attending Doctor, Department of Endodontics, Jinan Stomatological Hospital, No. 101, Jingliu Road, Jinan 250001, Shandong Province, China.
Telephone: +86-531-86261570 Fax: +86-531-86261570
Received: March 21, 2019
Peer-review started: March 23, 2019
First decision: April 18, 2019
Revised: April 23, 2019
Accepted: May 1, 2019
Article in press: May 1, 2019
Published online: June 26, 2019
Research background

Periodontal diseases are associated with various systemic diseases, including cardiovascular diseases such as stroke and atherosclerosis, which seriously affect the quality of life for patients.

Research motivation

At present, little is known about the potential mechanism of surgical treatment for periodontitis.

Research objectives

To explore the mechanism of surgical treatment for periodontitis based on the gene expression of peripheral blood mononuclear cells.

Research methods

Co-expression analysis, enrichment analysis, crosstalk analysis, and pivot regulatory factor prediction

Research results

A total of 337 genes related to periodontitis were clustered into 8 co-expression modules. These genes are mainly involved in G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger, and adenylate cyclase-modulating G-protein coupled receptor signaling pathway. In addition, 94 transcription factors (including NFKB1, SP1, and STAT3) and 1198 ncRNAs (including MALAT1, CRNDE, and ANCR) regulatory module genes were identified.

Research conclusions

The key factors we have identified affect the recovery of periodontitis after surgery through a variety of biological processes and signaling pathways

Research perspectives

The results of this study provide a new theoretical basis and individualized treatment direction for follow-up treatment.