Published online Jun 26, 2019. doi: 10.12998/wjcc.v7.i12.1383
Peer-review started: March 23, 2019
First decision: April 18, 2019
Revised: April 23, 2019
Accepted: May 1, 2019
Article in press: May 1, 2019
Published online: June 26, 2019
Periodontitis is a chronic inflammation of periodontal supporting tissue caused by local factors. Periodontal surgery can change the gene expression of peripheral blood mononuclear cells. However, little is known about the potential mechanism of surgical treatment for periodontitis.
To explore the potential molecular mechanism of surgical treatment for periodontitis.
First, based on the expression profiles of genes related to surgical treatment for periodontitis, a set of expression disorder modules related to surgical treatment for periodontitis were obtained by enrichment analysis. Subsequently, based on crosstalk analysis, we proved that there was a significant crosstalk relationship between module 3 and module 5. Finally, based on predictive analysis of multidimensional regulators, we identified a series of regulatory factors, such as endogenous genes, non-coding RNAs (ncRNAs), and transcription factors, which have potential regulatory effects on periodontitis.
A total of 337 genes related to surgical treatment for periodontitis were obtained, and 3896 genes related to periodontitis were amplified. Eight expression modules of periodontitis were obtained, involving the aggregation of 2672 gene modules. These modules are mainly involved in G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger, and adenylate cyclase-modulating G-protein coupled receptor signaling pathway. In addition, eight endogenous genes (including EGF, RPS27A, and GNB3) were screened by network connectivity analysis. Finally, based on this set of potential dysfunction modules, 94 transcription factors (including NFKB1, SP1, and STAT3) and 1198 ncRNAs (including MALAT1, CRNDE, and ANCR) were revealed. These core regulators are thought to be involved in the potential molecular mechanism of periodontitis after surgical treatment.
Based on the results of this study, we can show biologists and pharmacists a new idea to reveal the potential molecular mechanism of surgical treatment for periodontitis, and provide valuable reference for follow-up treatment programs.
Core tip: Based on the expression pattern and function of genes in peripheral blood mononuclear cells of periodontitis patients, key genes (including EGF, RPS27A, GNB3, etc.) were identified. Further, transcription factors (including NFKB1, SP1, STAT3, etc.,) and non-coding RNAs (including MALAT1, CRNDE, and ANCR) that significantly regulated gene co-expression modules were identified. Exploring the relationships between genes and regulators may reveal the potential molecular mechanism of surgical treatment for periodontitis.