Basic Study
Copyright ©The Author(s) 2019. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Clin Cases. Jun 26, 2019; 7(12): 1383-1392
Published online Jun 26, 2019. doi: 10.12998/wjcc.v7.i12.1383
Study on gene expression patterns and functional pathways of peripheral blood monocytes reveals potential molecular mechanism of surgical treatment for periodontitis
Jin-Ji Ma, Hong-Mei Liu, Xiang-Hua Xu, Li-Xin Guo, Qing Lin
Jin-Ji Ma, Jinan Stomatological Hospital Gaoxin Branch, Jinan 250001, Shandong Province, China
Hong-Mei Liu, Li-Xin Guo, Qing Lin, Department of Endodontics, Jinan Stomatological Hospital, Jinan 250001, Shandong Province, China
Xiang-Hua Xu, Department of Stomatology, Shandong Provincial Hospital, Jinan 250001, Shandong Province, China
Author contributions: Ma JJ and Liu HM contributed equally to this work. Ma JJ, Liu HM, and Xu XH designed the research; Ma JJ, Liu HM, and Guo LX performed the research; Xu XH and Lin Q analyzed the data; Ma JJ, Liu HM, and Lin Q wrote the paper.
Institutional review board statement: This study was reviewed and approved by the Institutional Review Board of Jinan Stomatological Hospital.
Conflict-of-interest statement: The authors declare no conflict of interest.
Data sharing statement: No additional data are available.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Corresponding author: Qing Lin, BSc, Attending Doctor, Department of Endodontics, Jinan Stomatological Hospital, No. 101, Jingliu Road, Jinan 250001, Shandong Province, China. cunl69338@163.com
Telephone: +86-531-86261570 Fax: +86-531-86261570
Received: March 21, 2019
Peer-review started: March 23, 2019
First decision: April 18, 2019
Revised: April 23, 2019
Accepted: May 1, 2019
Article in press: May 1, 2019
Published online: June 26, 2019
Abstract
BACKGROUND

Periodontitis is a chronic inflammation of periodontal supporting tissue caused by local factors. Periodontal surgery can change the gene expression of peripheral blood mononuclear cells. However, little is known about the potential mechanism of surgical treatment for periodontitis.

AIM

To explore the potential molecular mechanism of surgical treatment for periodontitis.

METHODS

First, based on the expression profiles of genes related to surgical treatment for periodontitis, a set of expression disorder modules related to surgical treatment for periodontitis were obtained by enrichment analysis. Subsequently, based on crosstalk analysis, we proved that there was a significant crosstalk relationship between module 3 and module 5. Finally, based on predictive analysis of multidimensional regulators, we identified a series of regulatory factors, such as endogenous genes, non-coding RNAs (ncRNAs), and transcription factors, which have potential regulatory effects on periodontitis.

RESULTS

A total of 337 genes related to surgical treatment for periodontitis were obtained, and 3896 genes related to periodontitis were amplified. Eight expression modules of periodontitis were obtained, involving the aggregation of 2672 gene modules. These modules are mainly involved in G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger, and adenylate cyclase-modulating G-protein coupled receptor signaling pathway. In addition, eight endogenous genes (including EGF, RPS27A, and GNB3) were screened by network connectivity analysis. Finally, based on this set of potential dysfunction modules, 94 transcription factors (including NFKB1, SP1, and STAT3) and 1198 ncRNAs (including MALAT1, CRNDE, and ANCR) were revealed. These core regulators are thought to be involved in the potential molecular mechanism of periodontitis after surgical treatment.

CONCLUSION

Based on the results of this study, we can show biologists and pharmacists a new idea to reveal the potential molecular mechanism of surgical treatment for periodontitis, and provide valuable reference for follow-up treatment programs.

Keywords: Peripheral blood mononuclear cells, Gene expression, Dysregulation module, Potential molecular mechanism, Gene expression pattern

Core tip: Based on the expression pattern and function of genes in peripheral blood mononuclear cells of periodontitis patients, key genes (including EGF, RPS27A, GNB3, etc.) were identified. Further, transcription factors (including NFKB1, SP1, STAT3, etc.,) and non-coding RNAs (including MALAT1, CRNDE, and ANCR) that significantly regulated gene co-expression modules were identified. Exploring the relationships between genes and regulators may reveal the potential molecular mechanism of surgical treatment for periodontitis.