Case Report Open Access
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World J Clin Cases. Apr 16, 2021; 9(11): 2619-2626
Published online Apr 16, 2021. doi: 10.12998/wjcc.v9.i11.2619
Cryptococcus infection with asymptomatic diffuse pulmonary disease in an immunocompetent patient: A case report
Yong Li, Lei Fang, Fang-Qun Chang, Fang-Zhou Xu, Yan-Bei Zhang
Yong Li, Lei Fang, Fang-Qun Chang, Fang-Zhou Xu, Yan-Bei Zhang, Department of Geriatric Respiratory and Critical Care, The First Affiliated Hospital of Anhui Medical University, Hefei 230000, Anhui Province, China
Yong Li, Department of Tuberculosis, Anhui Medical University Clinical College of Chest & Anhui Chest Hospital, Hefei 230000, Anhui Province, China
ORCID number: Yong Li (0000-0002-2091-3793); Lei Fang (0000-0001-6798-2932); Fang-Qun Chang (0000-0002-7951-8640); Fang-Zhou Xu (0000-0002-3101-8727); Yan-Bei Zhang (0000-0003-0551-4054).
Author contributions: Li Y, Chang FQ, and Xu FZ collected the clinical data and wrote the paper; Zhang YB and Fang L contributed to the design and revision of the paper.
Supported by The Colleges and Universities in Anhui Province Natural Science Research Projects, No. KJ2018A0208.
Informed consent statement: Informed written consent was obtained from the patient for publication of this report and any accompanying images.
Conflict-of-interest statement: The authors declare that they have no conflicts of interest to report.
CARE Checklist (2016) statement: The authors have read the CARE Checklist (2016), and the manuscript was prepared and revised according to the CARE Checklist (2016).
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See:
Corresponding author: Yan-Bei Zhang, MAMS, Chief Doctor, Professor, Department of Geriatric Respiratory and Critical Care, The First Affiliated Hospital of Anhui Medical University, No. 218 Jixi Street, Hefei 230000, Anhui Province, China.
Received: December 7, 2020
Peer-review started: December 7, 2020
First decision: December 21, 2020
Revised: January 9, 2021
Accepted: February 10, 2021
Article in press: February 10, 2021
Published online: April 16, 2021


Cryptococcus presenting as an opportunistic pathogen mainly affects immunocompromised patients, but the disseminated form of infection is rare among immunocompetent populations. The partial radiographic characteristics of pulmonary cryptococcosis mimic lung carcinoma, leading to unnecessary open chest exploratory surgery, and the lack of a gold-standard noninvasive diagnostic increases the risk of misdiagnosis. Positron emission tomography/computed tomography (PET/CT), a sensitive method for distinguishing malignant tumors, coupled with cryptococcal latex agglutination test showing a high positive rate may overcome these issues.


A 36-year-old man presented for general examination, without health complaints. Routine CT showed multiple pulmonary nodules and a mass with high maximum standardized uptake value. Initially, we suspected primary malignancy with hematogenous metastasis. Although his routine fungal analysis had been negative, subsequent CT-guided percutaneous core needle biopsy and histopathology examination indicated a diagnosis of pulmonary cryptococcosis. Fluconazole (200 mg/d) antifungal drug treatment was initiated, and 1 mo later the pulmonary mass had reduced in size markedly (on chest CT scan) without any complications.


Serologic and PET/CT examinations may not rule out cryptococcosis, and percutaneous lung puncture is critical under all circumstances.

Key Words: Cryptococcus, Immunocompetent, Positron emission tomography, Computed tomography, Cryptococcal latex agglutination test, Lung diseases, Fungal, Case report

Core Tip: Cryptococcosis is a systemic fungal infection, with presentation ranging from asymptomatic pulmonary involvement to meningitis and disseminated disease. Generally, cryptococcosis is considered an opportunistic infection in immunocompro-mised individuals, affecting persons with human immunodeficiency virus infection in particular. Mass-like and disseminated cryptococcosis lesions are rare in patients with normal immunity, and a negative result on the cryptococcal latex agglutination test may be related to low-grade virulence of the fungus. We describe here a case of pulmonary cryptococcosis that was likely caused by a low-virulence strain, presenting bilaterally distributed lesions on imaging and mimicking hematogenous metastasis.


Cryptococcosis is a systemic infection caused by two species of the encapsulated yeast-like fungus Cryptococcus: Cryptococcus gattii (C. gattii) and Cryptococcus neoformans (C. neoforman)[1]. These opportunistic fungal pathogens usually cause cryptococcosis in immunocompromised patients, with the respiratory system being the usual route of infection. Infections in immunocompetent persons are much rarer.

Positron emission tomography/computed tomography (PET/CT) is a useful tool for differentiating benign and malignant tissues. Higher uptake values of fluorodeoxyglucose on PET/CT reflect the characteristics of a malignant disease. Unfortunately, since the radiographic characteristics of pulmonary cryptococcosis mimic lung carcinoma, clinicians may order open chest exploratory surgery, with patients bearing the burden of undergoing an unnecessary invasive procedure[2].

Cases of pulmonary cryptococcosis, therefore, require further noninvasive testing for a definitive diagnosis; yet, no gold-standard noninvasive diagnostic exists and the risk of misdiagnosis remains large. Prior to a biopsy and histological assessment [by Grocott’s methenamine silver (GMS) staining], the most common suspected diagnoses are lung cancer, inflammatory granuloma, metastases from an extrathoracic malignancy, and pulmonary tuberculosis.

As described below for our case, CT-guided percutaneous core needle biopsy of a presumed lung carcinoma can be critical for a definitive diagnosis of Cryptococcus infection.

Chief complaints

A 34-year-old apparently immunocompetent Chinese man presented for general health examination. On routine CT, multiple pulmonary nodules and a mass were incidentally found.

History of present illness

The patient reported having had no symptoms of discomfort prior to presentation, including cough, sputum, chest pain, chills, or fever. He also denied tiring easily, having night sweats, and rapid weight loss.

History of past illness

The patient reported occupation as an engineer, smoking ten cigarettes a day for the past 3 years, and having no pets. He also had no history of allergies, hepatitis B, or diabetes mellitus, or any other significant medical history.

Physical examination

Physical examination upon admission found bilateral lungs to be clear, with no wheezing, rales, or rhonchi detected by auscultation. Temperature was 36.5 °C. Heart rate was 95 beats/min. Respiratory rate was 20 breaths/min. Blood pressure was 104/71 mmHg. There were no other remarkable findings.

Laboratory examinations

Test findings for liver function, renal function, and electrolytes were normal. The values of fasting blood glucose were within the normal range twice during hospitalization. All inflammatory biomarkers were also within the normal range. The result of human immunodeficiency virus (HIV) serology test was negative. Tumor markers in blood, including carcinoembryonic antigen, progastrin-releasing peptide, neuron-specific enolase, and cytokeratin 19-fragments (CYFRA 21.1), were all normal. Serology test for Cryptococcus antigen also gave negative results.

Imaging examinations

Due to the pulmonary lesions of unknown cause, PET/CT was arranged. The imaging showed multiple nodules in both lungs, enlarged right hilar lymph nodes, and an elliptic mass lesion without clear margin in the right lower lobe (Figure 1). The mass showed higher uptake values of 18F-fluorodeoxyglucose (FDG) and SUVmax of 16.4 in normal scans and 19.1 in delayed scans. The SUVmax for FDG uptake of those nodules was 6.8 in normal scans and 10.2 in delayed scans. The two enlarged lymph nodes in the right hilum gave 4.8 in normal scans and 6.9 in delayed scans.

Figure 1
Figure 1 Positron emission tomography/computed tomography showing bilateral lesions. An elliptic mass without clear margin was shown in the right lower lobe. The size of the mass was 3.46 cm × 2.39 cm. The enlarged right hilar lymph nodes showed a high value of SUVmax. There were also scattered nodules found in both lungs.
Further diagnostic work-up

Subsequently, the patient underwent CT-guided percutaneous core needle biopsy of the right lower-lobe mass. No fungi or remarkable bacteria were found in the puncture fluid smear.

Department of Pathology, Nanjing Gulou Hospital Affiliated to Medical School of Nanjing University

The pathologic consultation found granulomatous inflammation. GMS was positive and periodic acid-Schiff staining was negative (Figure 2). No evidence of malignancy was noted. In summary, a diagnosis of cryptococcal pneumonia was established.

Figure 2
Figure 2 Pathological examination revealed Cryptococcus infection. A: Grocott’s methenamine silver staining showed Cryptococcus spores by black staining (arrow); B: Periodic acid-Schiff staining was negative. Original magnification: × 400.

A diagnosis of pulmonary cryptococcosis was made according to the radiologic evidence of pulmonary lesions and positive histopathology, although the serology test for Cryptococcus antigen was negative.


Fluconazole (200 mg) per day orally.


After 1 mo and 3 mo of treatment, the pulmonary lesions were found to be markedly reduced in size on chest CT (Figures 3 and 4).

Figure 3
Figure 3 Chest computed tomography scan after 1 mo of antifungal treatment showing resolution of the bilateral lesions. The mass in the right lung was reduced markedly. A: The 25th floor scan; B: The 26th floor scan.
Figure 4
Figure 4 Chest computed tomography scan after 3 mo of antifungal treatment showing near complete disappearance of the nodules and infiltration distributed around the lesions. Only a small number of pulmonary cavities remained at this time. A: The 29th floor scan; B: The 30th floor scan.

Pulmonary cryptococcosis is prevalent in patients with compromised cell-mediated immunity, such as those with HIV infection or who have been subject to chemotherapy or other immunosuppressive therapy[3,4]. It is known that patients with HIV infection are more likely to have central nervous system involvement with the cryptococcosis disease than those without. In that group, the disease itself is associated with a high mortality rate. Cryptococcosis can also occur in individuals with normal immunity and causes severe nervous system infections[5], but such cases are rare. Table 1 illustrates the special features of our report.

Table 1 Cryptococcus infection in immunocompetent patients.
Sex, age
Medical history
Diagnostic modalities
Clinical presentation
1, this reportM36Good conditionCT-guided percutaneous core needle biopsyNonePulmonary nodules and a mass in both lungs; PET-CT showed the SUVmax of the cryptococcal lesions fluctuated from 4.8 to 19.1NegativeGranulomatous inflammation; GMS was positive and PSA was negativeFluconazole
2, Bavishi et al[20]F67Hypertension and cholelithiasisCT-guided percutaneous core needle biopsyRecurrent dry cough for 4 yrMultiple pulmonary nodules in both the lower lobesSerology cryptococcal antigen titer of 1: 32FMS staining was strongly positiveIntravenous amphotericin B for 2 d and then changed to fluconazole
3, Zhou et al[21]M44Good conditionBrochoscopy3-mo history of cough, hemoptysisPulmonary nodules in both lungs; PET-CT showed the SUVmax of the cryptococcal lesions fluctuated from 9.86 to 10.99Titer of more than 1: 1, 280GMS stain was positive; Culture of bronchoscopy with brush was positiveAmphotericin B
4, Marroni et al[22]F21Good conditionCT-guided percutaneous core needle biopsy Rigors, fever, dyspnoea, dry cough, and chest painA round mass in the lungPositive at a titre of 1: 256GMS and PSA staining was positive Fluconazole
5, Oliveira et al[5]M64Arterial hypertensionFiberoptic bronchoscopy with bronchoalveolar lavageFever, weakness, anorexia, headache, dyspnea, cough, purulent sputum production, and disorientationPulmonary spherical mass lesion, 5 cm in diameterThe CSF cryptococcal antigen titer was 1: 4096 with a serum titer of 1:2048MGG staininh was positiveAmphotericin B
6, Ruan et al[12]M68Good conditionSurgical drainageProgressive multiple abscesses, fever, lower extremity weakness, and urinary retentionPulmonary abscess formation and multiple destruction of vertebral bodiesNegativeCulture revealed; Cryptococcus neoformans; India ink stainingItraconazole; Fluorocytosine; Fluconazole

For our case, the patient was otherwise healthy and had no neurological symptoms. However, he presented bilaterally disseminated lesions in the lung, which is extremely rare for an asymptomatic immunocompetent person, and this increased the chance of mistaken and missed diagnosis. Interestingly, his cryptococcal latex agglutination test (CLAT) was also negative. In other reports in the literature, the positive rate for CLAT has been high, even exceeding 90%[6,7]. CLAT is also used to evaluate response to therapy for pulmonary cryptococcosis in patients without HIV infection[7].

Most cryptococcal strains are encapsulated by polysaccharides[8] and capsule-deficient C. neoformans may have reduced virulence[9]. A cryptococcal strain that has lost its capsular material can, however, trigger strong inflammatory reactions, which tends to lead to the formation of granulomas composed of histiocytes, giant cells, and lymphocytes. This is consistent with our pathological outcome. We speculated that our patient may have been infected with a low-virulence C. neoformans strain, thus causing the false negative CLAT result[10]. Certainly, other unknown possibilities cannot be excluded.

Of the two Cryptococcus species, C. neoformans is widespread, especially in pigeon feces or in soil contaminated with these feces, and it tends to cause disease in patients who are immunocompromised. In Taiwan, there is evidence to show a significant prevalence of C. gattii infection associated with the eucalyptus tree[11]. C. gattii usually causes disease in immunocompetent persons and behaves as a primary pathogen. The percentage of C. gattii infection in immunocompetent hosts is significantly higher than that of C. neoformans infection[12].

In 2018, the World Health Organization recommended that without the results of cerebrospinal fluid, rapid serum, plasma, or whole-blood cryptococcal antigen assays are the preferred diagnostic methods (either lateral flow assay or latex agglutination assay). Some studies have reported that CLAT has an insufficient sensitivity for C. gattii compared to the lateral flow assay, increasing the risk of delayed diagnosis and probability of misdiagnosis[13]. As such, in order to diagnose cryptococcosis, multiple detection methods should be applied to improve the accuracy of diagnosis.

C. gattii infection is more common in tropical areas[11], which is inconsistent with the geography of our patient’s residential area. During the subsequent visit, the patient told us that one of his neighbors kept pigeons, which supported our speculation that the patient was likely to have been infected with a low-virulence strain of C. neoformans.

The most common radiographic characteristics of pulmonary cryptococcosis consist of solitary pulmonary mass or nodules, combined patchy and nodular shadows, cavitation, and enlarged mediastinal lymph nodes[14]. However, the most common imaging finding of immunocompetent patients is single nodule. Mass-like and bronchopneumonic patterns are common in immunocompromised patients and rare in the patients with normal immunity[15]. PET/CT is a sensitive method for distinguishing malignant tumors from benign tissue, and it has gradually become a useful imaging technology for the differential diagnosis, staging, and follow-up of cancer[16]. The related SUVmax quantitatively measures the FDG uptake of a tumor.

Generally, the SUVmax value of 2.5 is used as the cut-off for differentiating benign lesions from malignant ones, as it is known to have the best sensitivity and specificity for such. However, the diagnostic accuracy of this value is not high in pulmonary infectious diseases, especially for those due to granulomatous diseases[17] such as in our patient. The SUVmax values for cases of pulmonary cryptococcosis may vary widely, indicating anywhere from mild to marked uptake. In previous reports, the SUVmax of the cryptococcal lesions fluctuate from 0.93 to 13.0[15,18], with most cases exceeding the 2.5 threshold. Nevertheless, few of the SUVmax values of pulmonary lesions reach 19.

The mechanism of increased FDG uptake in cryptococcal lesions may be related to the “respiratory burst”, which increases cellular glucose metabolism. Based on this principle, PET/CT can play a role in assessing the treatment of patients with fungal infection[19]. Therefore, diagnosis of malignant tumor by PET/CT should exclude the possibility of fungal infection. Our case was remarkable since we could not find evidence of immunocompromise in our patient; additional reports of such cases and bench-to-bedside studies will help to explain the development of masses and diffuse lesions in patients who are infected with a low-virulence strain (as we suspect our patient was).


We describe here an immunocompetent patient with pulmonary Cryptococcus infection, who manifested a mass and diffuse lesions in his lung while being asymptomatic. Although it is very rare, cryptococcosis must be included in the differential diagnosis of pulmonary lesions in patients without immunosuppression. It is important to recognize that pulmonary fungal infection can mimic metastases, even when the patient has been diagnosed with lung cancer by PET/CT. Furthermore, the potential for false negative results of fungal smears and serologic testing for cryptococcosis is another risk for misdiagnosis of this infectious disease. Thus, percutaneous lung puncture is critical under all circumstances in order to promote more timely initiation of appropriate treatment and avoidance of unnecessary surgery.


Manuscript source: Unsolicited manuscript

Specialty type: Respiratory system

Country/Territory of origin: China

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