Clinical and Translational Research
Copyright ©The Author(s) 2022. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Clin Cases. Jun 6, 2022; 10(16): 5165-5184
Published online Jun 6, 2022. doi: 10.12998/wjcc.v10.i16.5165
Circulating miR-627-5p and miR-199a-5p are promising diagnostic biomarkers of colorectal neoplasia
Dong-Yan Zhao, Lei Zhou, Teng-Fei Yin, Yuan-Chen Zhou, Ge-Yu-Jia Zhou, Qian-Qian Wang, Shu-Kun Yao
Dong-Yan Zhao, Ge-Yu-Jia Zhou, Shu-Kun Yao, Graduate school, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China
Dong-Yan Zhao, Ge-Yu-Jia Zhou, Shu-Kun Yao, Department of Gastroenterology, China-Japan Friendship Hospital, Beijing 100029, China
Lei Zhou, Department of General Surgery, China-Japan Friendship Hospital, Beijing 100029, China
Teng-Fei Yin, Yuan-Chen Zhou, Qian-Qian Wang, Graduate school, Peking University China-Japan Friendship School of Clinical Medicine, Beijing 100029, China
Author contributions: Zhao DY designed and performed the study, analyzed the data, and drafted the manuscript; Zhou L and Yin TF collected blood samples from subjects, and provided guidance on experimental procedures; Zhou YC, Zhou GYJ, and Wang QQ collected the clinical data and colorectal samples from the subjects; Yao SK designed the study, supervised the study performance, revised the manuscript, and obtained the funding; all authors read and approved the final manuscript.
Supported by National Key Development Plan for Precision Medicine Research, No. 2017YFC0910002.
Institutional review board statement: This study was approved by the Ethics Committee of China-Japan Friendship Hospital (No. 2018-116-K85).
Informed consent statement: All study participants, or their legal guardian, provided informed written consent prior to study enrollment.
Conflict-of-interest statement: There are no conflicts of interest to report.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Shu-Kun Yao, PhD, Professor, Department of Gastroenterology, China-Japan Friendship Hospital, Graduate school, Chinese Academy of Medical Sciences and Peking Union Medical College, No. 2 Yinghua East Road, Chaoyang District, Beijing 100029, China. shukunyao@126.com
Received: October 22, 2021
Peer-review started: October 22, 2021
First decision: December 27, 2021
Revised: December 29, 2021
Accepted: March 15, 2022
Article in press: March 15, 2022
Published online: June 6, 2022
Core Tip

Core Tip: This report is the first on the diagnostic usefulness of circulating miR-627-5p and miR-199a-5p in patients with colorectal neoplasms. We identified that serum levels of miR-627-5p and miR-199a-5p were markedly elevated in patients with colorectal neoplasia and appeared to be novel biomarkers for the non-invasive screening of colorectal neoplasia. An integrated model combining miR-199a-5p and miR-627-5p obtained a better discriminative capacity than each miRNA alone. Additionally, miR-627-5p and miR-199a-5p had different expression levels in the serum of cancer patients before and after surgery and in vitro gain-of-function experiments demonstrated that both microRNAs played crucial roles in regulating the progression and invasion of colorectal cancer cells.