Clinical and Translational Research
Copyright ©The Author(s) 2020. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Clin Cases. Aug 6, 2020; 8(15): 3197-3208
Published online Aug 6, 2020. doi: 10.12998/wjcc.v8.i15.3197
Overexpression of AMPD2 indicates poor prognosis in colorectal cancer patients via the Notch3 signaling pathway
Qi-Zhong Gao, Yan Qin, Wei-Jia Wang, Bo-Jian Fei, Wei-Feng Han, Jian-Qiang Jin, Xiang Gao
Qi-Zhong Gao, Bo-Jian Fei, Wei-Feng Han, Department of Gastrocolorectal Surgery, Affiliated Hospital of Jiangnan University, Wuxi 214100, Jiangsu Province, China
Yan Qin, Wei-Jia Wang, Jian-Qiang Jin, Department of Pathology, Affiliated Hospital of Jiangnan University, Wuxi 214000, Jiangsu Province, China
Xiang Gao, Department of Oncology, Affiliated Hospital of Jiangnan University, Wuxi 214062, Jiangsu Province, China
Author contributions: Gao QZ and Qin Y designed the research; Gao QZ performed the research. Qin Y and Jin JQ reviewed the surgical pathology; Fei BJ and Han WF analyzed the data; Gao QZ and Gao X wrote the paper; all authors read and approved the final manuscript.
Supported by the Wuxi Young Medical Talents, Jiangsu Province, China, No. QNRC063.
Institutional review board statement: This study was approved by the Ethics Committee of Affiliated Hospital of Jiangnan University and was performed in accordance with the ethical standards of the Declaration of Helsinki and its later amendments.
Clinical trial registration statement: This registration policy applies to prospective, randomized, controlled trials only.
Informed consent statement: All study participants, or their legal guardian, provided informed written consent prior to study enrollment.
Conflict-of-interest statement: The authors declare that they have no competing interests.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Corresponding author: Xiang Gao, MD, Academic Research, Department of Oncology, Affiliated Hospital of Jiangnan University, No. 200 Huihe Road, Wuxi 214062, Jiangsu Province, China. 13606189128@139.com
Received: March 24, 2020
Peer-review started: March 24, 2020
First decision: April 24, 2020
Revised: May 26, 2020
Accepted: July 15, 2020
Article in press: July 15, 2020
Published online: August 6, 2020
ARTICLE HIGHLIGHTS
Research background

As an important gastrointestinal tumor, colorectal cancer (CRC) is often accompanied by abnormal energy metabolism in the case of malignant progression and poor prognosis.

Research motivation

As the abnormal energy metabolism in tumor cells has received considerable attention, we have combined the tissue type and biological characteristics of CRC. With AMPD2 as the entry point for research, a variety of scientific methods can be integrated for research.

Research objectives

Clarify the role of AMPD2 in CRC and study the pathway and prognostic value of its role.

Research methods

RNAseq data analysis of the TCGA-COAD data set, GO and KEGG analysis. Western blot was used to detect the expression of Notch3-related pathway proteins, as well as clinical pathological samples combined with patient prognosis analysis.

Research results

AMPD2 is commonly overexpressed in TCGA CRC tissues, and the function of AMPD2 is associated with the Notch signaling pathway in CRC in vitro. In the CRC cohort, as indicated by tissue microarray analysis, high expression of AMPD2 protein was correlated with advanced depth of tumor and poor differentiation.

Research conclusions

The mRNA and protein levels of AMPD2 are significantly highly expressed in CRC tissues. The high expression of AMPD2 has a positive relationship with activation of the Notch3 signaling pathway. In patients with CRC, those with high expression of AMPD2 have a poor prognosis.

Research perspectives

In the future, we plan to further study the biological mechanism of AMPD2 stimulation of abnormal lipid metabolism in CRC cells.