Protective effect of some vitamins against the toxic action of ethanol on liver regeneration induced by partial hepatectomy in rats

Table 1 Mortality and body weight parameters in each experimental group after seven days of treatment partial hepatectomy (PH), ethanol (EtOH) and some vitamins

Group	Mortality (%)	Initial body Weight’ (g)	Final body weight’ (g)	Differential between final and initial weight (g)
Sham	0/30 (0)	259 ± 10	274 ± 1	15
PH	0/30 (0)	250 ± 11	267 ± 10	17
PH-EtOH	22/30 (73.3)a	255 ± 10	276 ± 15	21
PH-EtOH-VA	16/30 (53.3)e	255 ± 12	270 ± 12	15
PH-EtOH-VC	10/30 (33.3)e	260 ± 12	275 ± 10	15
PH-EtOH-VE	8/30 (26.6)e	242 ± 10	258 ± 11	16

¹Values are the mean ± SE of survivors for each experimental group (n = 8-22) as it corresponds. VA: Vitamin A; VC: Vitamin C; VE: Vitamin E.

^aP < 0.05 vs the sham group;

^eP < 0.05 vs hepatectomized + ethanol group.

Table 2 Evaluation of liver regeneration parameters in each experimental group after seven days of treatment with partial hepatectomy (PH), ethanol (EtOH) and vitamins. Experimental designs are described in material and methods section

Group	Restitution liver mass’ (%)	Resected liver mass’ (g)	Final liver weight’ (g)	Liver/body ratio’ (%)	mgDNA/g liver1
Sham	None	None	9.41 ± 0.3	3.41 ± 0.2	2.1 ± 0.2
PH	83.95 ± 3.0	6.9 ± 0.3	8.27 ± 0.3	3.09 ± 0.3a	4.1 ± 0.4a
PH-EtOH	54.89 ± 2.0c	6.8 ± 0.4	5.33 ± 0.2c	1.93 ± 0.1ac	2.9 ± 0.1ac
PH-EtOH VA	62.90 ± 3.9ce	7.1 ± 0.3	6.38 ± 0.4c	2.36 ± 0.3ace	3.4 ± 0.2ace
PH-EtOH-VC	79.00 ± 2.0c	7.0 ± 0.5	7.90 ± 0.2e	2.87 ± 0.2ae	4.0 ± 0.3ae
PH-EtOH-VE	81.00 ± 3.0c	6.8 ± 0.2	7.86 ± 0.3e	3.03 ± 0.3ae	3.9 ± 0.4ae

¹Values are the mean ± SE of survivors for each experimental group (n = 8-22) as it corresponds. Vitamin A (VA); Vitamin C (VC); Vitamin E (VE).

^aP < 0.05 vs the sham group;

^cP < 0.05 vs the hepatectomized group;

^eP < 0.05 vs hepatectomized + ethanol group.

Table 3 Albumin and bilirubin levels on serum from rats after seven days of partial hepatectomy (PH) and daily ingestion of ethanol and application of vitamins A, C or E

Group	Albumin’ (g/dL)	Bilirubin’ (mg/dL)
Sham	4.5 ± 0.2	0.42 ± 0.01
PH	4.7 ± 0.3	0.40 ± 0.02
PH-EtOH	3.1 ± 0.4ac	1.72 ± 0.04ac
PH-EtOH VA	3.5 ± 0.3ac	1.91 ± 0.06ac
PH-EtOH-VC	4.2 ± 0.2e	0.45 ± 0.01e
PH-EtOH-VE	4.6 ± 0.3e	0.29 ± 0.03ace

¹Values are the mean ± SE of survivors for each experimental group (n = 8-22) as it corresponds. vitamin A (VA); Vitamin C (VC); vitamin E (VE).

^aP < 0.05 vs the sham group;

^cP < 0.05 vs the hepatectomized group;

^eP < 0.05 vs hepatectomized + ethanol group.