Effect of 5-2Aza-2'-deoxycytidine on the P16 tumor suppressor gene in hepatocellular carcinoma cell line HepG2

Figure 1 Immunohistochemical demonstration of p16 protein expression in HepG2 cell line before treatment with 5-Aza-cdR. SABC × 200

Figure 2 Immunohistochemical demonstration of p16 protein expression in HepG2 cell line after treatment with 5-Aza-cdR. SABC × 200

Figure 3 Effects of 5-Aza-cdR on the p16 mRNA expression in HepG2 cell line. Cells were treated with 5 × 10^-7 M 5-Aza-cdR for 24 hours; after culturing for 9 d, p16 mRNA were determined by RT-PCR. U: untreated T: treated with 5-Aza-CdR

Figure 4 Detection of aberrant methylation of the p16 gene in HCC cell line HepG2. Lane M: molecular weight marker; Lane 1: Neither treated with 5-aza-cdR nor digested with Hpa II or Msp I; Lane 2: Untreated with 5-aza-cdR, but digested with Hpa II; Lane 3: Untreated with 5-Aza-cdR, but digested with Msp I; Lane 4: Treated with 5-aza-cdR, but not digested with Hpa II or Msp I; Lane 5: Treated with 5-aza-cdR, and digested with Hpa II; Lane 6: Treated with 5-aza-cdR, and digested with Msp I.