Randomized Controlled Trial
Copyright ©2014 Baishideng Publishing Group Inc.
World J Gastroenterol. Aug 7, 2014; 20(29): 10158-10165
Published online Aug 7, 2014. doi: 10.3748/wjg.v20.i29.10158
Figure 1
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Figure 1 Methotrexate-induced effects on hepatic structure in rats. Photomicrographs of representative liver tissues are shown for the control group (A) and model group (B-D). A: Normal histological appearence of H-E stained control liver tissues, showing vena centralis (VC) at × 40; B: Abnormal histological appearance of H-E stained model liver tissues, showing hepatocytes with eosinophilic cytoplasm around the portal area (arrows) at × 40; C: Inflammatory cells in the portal area (arrows) and congestion at × 40 (arrowhead); and D: Hydropic degeneration (vacuolization/cellular swelling) in hepatocytes at × 40.
Figure 2
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Figure 2 Methotrexate-induced effects on glycogen storage in hepatocytes. Photomicrographs of representative liver tissues are shown for the control group (A), model group (B, C), and antioxidant treatment groups (D-F). Normal histological appearence of PAS stained control liver tissue showin glycogen within the hepatocytes (magenta color) at × 20 (A). Decreased glycogen storage in hepatocytes is shown in PAS stained model liver tissues at × 10 (B) and × 20 (C) . Differential effects on MTX-reduced glycogen storage are shown in PAS stained liver tissues from the MTX + NAC group at × 20 (D), MTX + AMF group at × 20 (E), and MTX + ASC group at × 20 (F). VC: Vena centralis; MTX: Methotrexate; NAC: N-acetyl cysteine; AMF: Amifostine; ASC: Ascorbic acid; PAS: Periodic acid-Schiff.
Figure 3
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Figure 3 Methotrexate-induced structural damage to hepatic tissues in rats treated with antioxidant agents. A: Photomicrographs of representative H-E stained liver tissues are shown at × 40; B:The MTX + NAC group showed radial hepatocytes in the region from the central vein to the periphery; C: The MTX + AMF group showed sinusoidal dilatation (arrows) that was similar to that observed in the untreated model group. VC: Vena centralis; H-E: Hematoxylin-eosin; MTX: Methotrexate; NAC: N-acetyl cysteine; AMF: Amifostine.