Cytoprotective effects of amifostine, ascorbic acid and N-acetylcysteine against methotrexate-induced hepatotoxicity in rats

Figure 2 Methotrexate-induced effects on glycogen storage in hepatocytes. Photomicrographs of representative liver tissues are shown for the control group (A), model group (B, C), and antioxidant treatment groups (D-F). Normal histological appearence of PAS stained control liver tissue showin glycogen within the hepatocytes (magenta color) at × 20 (A). Decreased glycogen storage in hepatocytes is shown in PAS stained model liver tissues at × 10 (B) and × 20 (C) . Differential effects on MTX-reduced glycogen storage are shown in PAS stained liver tissues from the MTX + NAC group at × 20 (D), MTX + AMF group at × 20 (E), and MTX + ASC group at × 20 (F). VC: Vena centralis; MTX: Methotrexate; NAC: N-acetyl cysteine; AMF: Amifostine; ASC: Ascorbic acid; PAS: Periodic acid-Schiff.