Brief Article
Copyright ©2010 Baishideng.
World J Gastroenterol. Mar 14, 2010; 16(10): 1285-1292
Published online Mar 14, 2010. doi: 10.3748/wjg.v16.i10.1285
Figure 1
Figure 1 ED2 immunocytochemistry staining of cultured Kupffer cells. More than 95% are ED2 positive. Cells adhere to plastic and exhibit a spread out morphology and irregular shape (× 400).
Figure 2
Figure 2 HO-1 overexpression ameliorates hepatic I/R injury. A: The expression of hepatic HO-1 was evaluated by RT-PCR before liver transplantation. β-actin was used as an internal control. Decreased expression of HO-1 mRNA was detected 24 h after ZnPP treatment. CoPP treatment increased HO-1 mRNA (n = 4/group). bP < 0.001; cP < 0.0001; B: Hepatocellular function. Serum ALT/AST levels (U/L) were higher in the ZnPP group as compared with controls. Significantly lower serum transaminase levels were seen in the CoPP group (n = 8/group). aP < 0.05; dP < 0.01; C: Representative photomicrographs of rat livers following 6 h reperfusion. Livers in ZnPP group show severe lobular distortion, sinusoidal congestion, ballooning, hepatocyte necrosis. Livers pretreated with CoPP exhibit good preservation of lobular architecture. Original magnification, × 400; HE stain.
Figure 3
Figure 3 Concentration of IL-6 and TNF-α in cultured supernatant. Detection of cytokines at 48 h of Kupffer cell culture. ZnPP treatment increased cytokine levels of IL-6 (A) and TNF-α (B) were assayed. Significantly lower cytokine levels were detected in the CoPP group as compared with controls. The cytokine levels in cultured supernatant were measured by ELISA technique as described in Materials and methods (n = 5/group). aP < 0.05, bP < 0.01.
Figure 4
Figure 4 HO-1 and CD14 expression levels of cultured Kupffer cells. A: HO-1 mRNA expression levels of Kupffer cells were significantly downregulated in the ZnPP group and upregulated in the CoPP group. ZnPP treatment CD14 mRNA expression was expressed as relative increase compared with control. CoPP pretreated was lower (n = 4/group); B: Western blot analysis for CD14 protein of Kupffer cells. β-actin was used as internal control. CD14 protein expression levels increased in the ZnPP group and decreased in the CoPP group (n = 4/group). aP < 0.05, bP < 0.01.