Heme oxygenase-1 protects donor livers from ischemia/reperfusion injury: The role of Kupffer cells

Figure 3 Concentration of IL-6 and TNF-α in cultured supernatant. Detection of cytokines at 48 h of Kupffer cell culture. ZnPP treatment increased cytokine levels of IL-6 (A) and TNF-α (B) were assayed. Significantly lower cytokine levels were detected in the CoPP group as compared with controls. The cytokine levels in cultured supernatant were measured by ELISA technique as described in Materials and methods (n = 5/group). ^aP < 0.05, ^bP < 0.01.