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©The Author(s) 2017. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Jun 28, 2017; 23(24): 4341-4353
Published online Jun 28, 2017. doi: 10.3748/wjg.v23.i24.4341
Published online Jun 28, 2017. doi: 10.3748/wjg.v23.i24.4341
Indole phytoalexin derivatives induce mitochondrial-mediated apoptosis in human colorectal carcinoma cells
Viera Tischlerova, Martin Kello, Jan Mojzis, Department of Pharmacology, Faculty of Medicine, Pavol Jozef Safarik University, 04011 Kosice, Slovak Republic
Mariana Budovska, Department of Organic Chemistry, Institute of Chemistry, Faculty of Science, Pavol Jozef Safarik University, 04154 Kosice, Slovak Republic
Author contributions: Tischlerova V designed the procedure; conducted in vitro experiments; wrote the manuscript; Kello M designed the procedure, conducted in vitro experiments; Budovska M synthesized the studied compounds; Mojzis J revised the paper and gave final approval.
Supported by the Slovak Grant Agency for Science VEGA , No. 1/0322/14 and No. 1/0753/17 .
Institutional review board statement: No human or animal subjects were involved in our experiments.
Institutional animal care and use committee statement: No animal subjects were involved in our experiments.
Conflict-of-interest statement: All authors declared that there was no conflict of interest related to this study.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Jan Mojzis, Professor, DVM, DSc, Department of Pharmacology, Faculty of Medicine, Pavol Jozef Safarik University, 04011 Kosice, Slovak Republic. jan.mojzis@upjs.sk
Telephone: +421-55-2343485 Fax: +421-55-2343485
Received: January 26, 2017
Peer-review started: February 2, 2017
First decision: February 15, 2017
Revised: March 27, 2017
Accepted: May 19, 2017
Article in press: May 19, 2017
Published online: June 28, 2017
Peer-review started: February 2, 2017
First decision: February 15, 2017
Revised: March 27, 2017
Accepted: May 19, 2017
Article in press: May 19, 2017
Published online: June 28, 2017
Core Tip
Core tip: In this study we inform about our first results obtained from cell proliferation assays, flow cytometry analysis and Western blot analysis of HCT116 cells after treatment with compound K-453, which belongs to a group of synthetic derivatives of indole phytoalexins originally isolated from cruciferous vegetables. This study demonstrates that our compound induces the mitochondrial pathway of apoptosis and that the antiproliferative effect of our substance may be linked to inhibition of several signaling pathways. Our results provide a rationale for further in vivo anticancer efficacy studies.