Bai Y, Li LR, Wang JD, Chen Y, Jin JF, Zhang ZS, Zhou DY, Zhang YL. Expression of Helicobacter pylori Hsp60 protein and its immunogenicity. World J Gastroenterol 2003; 9(12): 2711-2714 [PMID: 14669319 DOI: 10.3748/wjg.v9.i12.2711]
Corresponding Author of This Article
Dr. Ya-Li Zhang, PLA Institute for Digestive Medicine, Nanfang Hospital, First Military Medical University, Guangzhou 510515, Guangdong Province, China. baiyang1030@hotmail.com
Article-Type of This Article
H Pylori
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World J Gastroenterol. Dec 15, 2003; 9(12): 2711-2714 Published online Dec 15, 2003. doi: 10.3748/wjg.v9.i12.2711
Expression of Helicobacter pylori Hsp60 protein and its immunogenicity
Yang Bai, Liang-Ren Li, Ji-De Wang, Ye Chen, Jian-Feng Jin, Zhao-Shan Zhang, Dian-Yuan Zhou, Ya-Li Zhang
Yang Bai, Liang-Ren Li, Ji-De Wang, Ye Chen, Dian-Yuan Zhou, Ya-Li Zhang, PLA Institute for Digestive Medicine, Nanfang Hospital, First Military Medical University, Guangzhou 510515, Guangdong Province, China
Jian-Feng Jin, Chemistry University of Beijing, Beijing 100071, China
Zhao-Shan Zhang, Institute of Biotechnology, Academy of Military Medical Science, Beijing 100071, China
Author contributions: All authors contributed equally to the work.
Supported by the National Natural Science Foundation of China, No.30270078
Correspondence to: Dr. Ya-Li Zhang, PLA Institute for Digestive Medicine, Nanfang Hospital, First Military Medical University, Guangzhou 510515, Guangdong Province, China. baiyang1030@hotmail.com
Telephone: +86-20-61641532
Received: March 12, 2003 Revised: May 2, 2003 Accepted: May 16, 2003 Published online: December 15, 2003
Abstract
AIM: To express Hsp60 protein of H pylori by a constructed vector and to evaluate its immunogenicity.
METHODS: Hsp60 DNA was amplified by PCR and inserted into the prokaryotie expression vector pET-22b (+), which was transformed into BL21 (DE3) E.coli strain to express recombinant protein. Immunogenicity of expressed Hsp60 protein was evaluated with animal experiments.
RESULTS: DNA sequence analysis showed Hsp60 DNA was the same as GenBank’s research. Hsp60 recombinant protein accounted for 27.2% of the total bacterial protein, and could be recognized by the serum from H pylori infected patients and Balb/c mice immunized with Hsp60 itself.
CONCLUSION: Hsp60 recombinant protein might become a potential vaccine for controlling and treating H pylori infection.
