Published online Dec 15, 2003. doi: 10.3748/wjg.v9.i12.2711
Revised: May 2, 2003
Accepted: May 16, 2003
Published online: December 15, 2003
AIM: To express Hsp60 protein of H pylori by a constructed vector and to evaluate its immunogenicity.
METHODS: Hsp60 DNA was amplified by PCR and inserted into the prokaryotie expression vector pET-22b (+), which was transformed into BL21 (DE3) E.coli strain to express recombinant protein. Immunogenicity of expressed Hsp60 protein was evaluated with animal experiments.
RESULTS: DNA sequence analysis showed Hsp60 DNA was the same as GenBank’s research. Hsp60 recombinant protein accounted for 27.2% of the total bacterial protein, and could be recognized by the serum from H pylori infected patients and Balb/c mice immunized with Hsp60 itself.
CONCLUSION: Hsp60 recombinant protein might become a potential vaccine for controlling and treating H pylori infection.