Basic Research
Copyright ©The Author(s) 2002. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Dec 15, 2002; 8(6): 1112-1116
Published online Dec 15, 2002. doi: 10.3748/wjg.v8.i6.1112
Effect of matrine on Kupffer cell activation in cold ischemia reperfusion injury of rat liver
Xin-Hua Zhu, Yu-Dong Qiu, Hao Shen, Ming-Ke Shi, Yi-Tao Ding
Xin-Hua Zhu, Yu-Dong Qiu, Ming-Ke Shi, Yi-Tao Ding, Department of Hepatobiliary Surgery, Affiliated Drum Tower Hospital, Medical Department of Nanjing University, Nanjing 210008, Jiangsu Province, China
Hao Shen, Biochemistry Department of Nanjing University, Nanjing 210008, Jiangsu Province, China
Author contributions: All authors contributed equally to the work.
Supported by the Chinese Medicine Administration Bureau of Jiangsu Province, No SZ 9902
Correspondence to: Dr. Xin-Hua Zhu, Department of Hepatobiliary Surgery, Affiliated Drum Tower Hospital, Medical College of Nanjing University, Zhongshang Road 321, Nanjing 210008, Jiangsu Province, China. drzhu@elong.com
Telephone: +86-25-3304616 Ext. 11601 Fax: +86-25-3317016
Received: June 1, 2002
Revised: June 19, 2002
Accepted: June 26, 2002
Published online: December 15, 2002
Abstract

AIM: To study the effect of matrine on activation of Kupffer cell during cold ischemia and reperfusion injury in rat orthotopic liver transplantation (OLT).

METHODS: 168 syngeneic SD rats were randomly divided into four groups: untreated group, small-dose treated group, large-dose treated group and sham operation group. After 5 h of preservation in Ringer’s (LR) solution, orthotopic implantation of the donor liver was performed. At 1 h, 2 h, 4 h and 24 h after reperfusion of the portal vein, 6 rats were killed in each group to collect the serum and the liver for assay and pathology.

RESULTS: Matrine markedly inhibited the activation of Kupffer cells and their release of tumor necrosis factor (TNF). TNF cytotoxicity level at 2 h decreased significantly by matrine treatment (7.94 ± 0.42, 2.39 ± 0.19 and 2.01 ± 0.13 U/mL, respectively; P < 0.01), so did the other three time points. The level of hylluronic acid (HA) and alanine transaminase (ALT) decreased significantly in both treated groups, and matrine treatment markedly ameliorated focal necrosis of hepatocytes, inflammatory cells aggregating, rounding and detachment of sinusoidal endothelial cells (SEC). And no significant difference was observed between the treated groups.

CONCLUSION: Matrine can inhibit the activation of Kupffer cell and prevent the donor liver from cold preservation and reperfusion injury in rat orthotopic liver transplantation.

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