Published online Apr 28, 2019. doi: 10.3748/wjg.v25.i16.1964
Peer-review started: February 20, 2019
First decision: March 13, 2019
Revised: March 21, 2019
Accepted: March 24, 2019
Article in press: March 25, 2019
Published online: April 28, 2019
The circular RNA circ-PRKCI is an endogenous non-coding RNA that forms a covalently closed ring after reverse splicing, which plays a key role in the occurrence and development of multiple digestive system tumors.
To investigate the role and mechanism of circ-PRKCI in the occurrence and development of hepatocellular carcinoma (HCC).
This study used real-time polymerase chain reaction to detect the expression of circ-PRKCI in tumor tissues, tumor adjacent tissues, and blood in patients with HCC and other digestive system tumor cells. A series of functional tests were performed to explore whether circ-PRKCI affects the growth of HCC cells and what is its mechanism in HCC. Meanwhile, fluorescence in situ hybridization was used to detect the subcellular localization of circ-PRKCI. Survival analysis was performed to predict the correlation between circ-PRKCI and the prognosis of HCC. Chi-square test and t-test were performed for statistical analyses.
The level of circ-PRKCI was significantly higher in HCC tissues than in tumor adjacent tissues, and in HCC cell lines than in cells lines of esophageal, liver, stomach, and colon cancers. A series of functional tests showed that circ-PRKCI substantially inhibited cell apoptosis and promoted cell invasion. It was found that circ-PRKCI can act as the sponge of miRNA-545 to reduce the expression of AKT3 protein. Moreover, the result of survival analysis showed that circ-PRKCI target gene E2F7 can reduce liver cancer patients’ survival rate. And clinical data suggested that the distribution of circ-PRKCI rose with the depth of invasion, lymph node metastasis, distant metastasis, and TNM stage, indicating that circ-PRKCI may affect the survival and prognosis of patients with HCC by regulating E2E7.
This study explores the role and mechanism of circ-PRKCI in HCC, which provides a new research direction and theoretical basis for the treatment of HCC.
Core tip: The circular RNA circ-PRKCI plays an important role in a variety of digestive system tumors. This study explored the role and mechanism of circ-PRKCI in hepatocellular carcinoma. The results of the study showed that: (1) significant high circ-PRKCI expression is present in liver cancer progression; (2) circ-PRKCI promotes the invasion of liver cancer cells; (3) circ-PRKCI can function as a ceRNA to regulate protein kinase B signaling pathway along with miRNA-545; and (4) circ-PRKCI target gene E2F7 may affect the survival and prognosis of patients with liver cancer. This study provides a new research direction and theoretical basis for the treatment of HCC.