Published online Apr 28, 2019. doi: 10.3748/wjg.v25.i16.1964
Peer-review started: February 20, 2019
First decision: March 13, 2019
Revised: March 21, 2019
Accepted: March 24, 2019
Article in press: March 25, 2019
Published online: April 28, 2019
Digestive system tumors have high morbidity and mortality and account for a large proportion of all tumors. It has been confirmed that circRNAs are important in the occurrence and development of some digestive system tumors, such as gastric cancer, liver cancer, and colon cancer.
Does circ-PRKCI affect the occurrence and development of digestive tract tumors, especially hepatocellular carcinoma?
To investigate the role and molecular mechanism of circ-PRKCI in the progression of hepatocellular carcinoma.
This study used quantitative real-time PCR, Western blot, knockdown and overexpression experiments, fluorescence in situ hybridization, survival analysis, and statistical analyses.
The level of circ-PRKCI was significantly higher in HCC tissues than in adjacent tissues. Circ-PRKCI substantially inhibited cell apoptosis and promoted cell invasion. It was found that circ-PRKCI can act as the sponge of miRNA-545 to reduce the expression of AKT3 protein. Circ-PRKCI target gene E2F7 can reduce liver cancer patients’ survival rate. Circ-PRKCI was positively correlated with the depth of invasion, lymph node metastasis, distant metastasis, and TNM stage.
Circ-PRKCI is significantly involved in the development of hepatocellular carcinoma.
This study provides a new research direction and theoretical basis for the treatment of hepatocellular carcinoma.