Published online Mar 14, 2017. doi: 10.3748/wjg.v23.i10.1771
Peer-review started: November 23, 2016
First decision: February 19, 2016
Revised: January 22, 2017
Accepted: February 7, 2017
Article in press: February 8, 2017
Published online: March 14, 2017
To establish a severe acute cholangitis (SAC) model in mice.
Cholecystic catheterization was performed under the condition of bile duct ligation (BDL). Trans-cholecystic injection of lipopolysaccharide (LPS) was defined as the SAC animal model. Sham operation group, intraperitoneal injection of LPS without BDL group, intraperitoneal injection of LPS with BDL group and trans-cholecystic injection of normal saline with BDL group were defined as control groups. The survival rates and tissue injuries in liver, lungs and kidney were evaluated.
Mice in the SAC group showed a time-dependent mortality and much more severe tissue injuries in liver, lungs and kidney, compared with other groups. However, relieving biliary obstruction could effectively reduce mortality and attenuate liver injury in the SAC mouse model.
Trans-cholecystic injection of LPS under the condition of biliary obstruction could establish a repeatable and reversible mouse model of SAC.
Core tip: Severe acute cholangitis (SAC) is a severe biliary tract infection. Although mice are the most common experimental animal and have a similar anatomical construction of bile ducts to humans, there is still no valid study on establishing a SAC model in mice. To study SAC more easily and exactly, we established a repeatable and reversible mouse model of SAC through cholecystic catheterization under the condition of bile duct ligation.