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Human papillomavirus DNA and P16INK4A expression in concurrent esophageal and gastric cardia cancers
Guang-Cheng Ding, Jing-Li Ren, Fu-Bao Chang, Ji-Lin Li, Ling Yuan, Xin Song, Sheng-Li Zhou, Tao Guo, Zong-Min Fan, Yi Zeng, Li-Dong Wang
Guang-Cheng Ding, Tao Guo, Department of Gastroenterology, The First Affiliated Hospital and The Fifth Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, Henan Province, China
Jing-Li Ren, Xin Song, Sheng-Li Zhou, Zong-Min Fan, Li-Dong Wang, Henan Key Laboratory for Esophageal Cancer Research, Department of Gastroenterology, The First Affiliated Hospital, College of Basic Medicine, Zhengzhou University, Zhengzhou 450052, Henan Province, China
Fu-Bao Chang, Department of Thoracic Surgery, Linzhou Center Hospital, Linzhou 456500, Henan Province, China
Ji-Lin Li, Department of Pathology, Yaocun Esophageal Cancer Hospital, Linzhou 456500, Henan Province, China
Ling Yuan, Department of Oncoradiotherapy, Henan Province Tumor Hospital, Zhengzhou 450003, Henan Province, China
Yi Zeng, Institute of Virology, Chinese Academy of Preventive Medicine, Beijing 100052, China
Author contributions: Ding GC and Wang LD designed the study and drafted the article; Chang FB, Li JL, Yuan L, Song X, Zhou SL and Fan ZM collected and prepared the CC samples; Guo T performed the DNA extraction and interpreted the data; Ren JL performed the immunohistochemical staining and statistical analysis; Wang LD and Zeng Y critically revised the manuscript.
Correspondence to: Dr. Li-Dong Wang, Henan Key Laboratory for Esophageal Cancer Research, Department of Gastroenterology, The First Affiliated Hospital, College of Basic Medicine, Zhengzhou University, Zhengzhou 450052, Henan Province, China. ldwang2007@126.com
Telephone: +86-371-66658335 Fax: +86-371-66658335
Received: July 9, 2010
Revised: September 20, 2010
Accepted: September 27, 2010
Published online: December 14, 2010
AIM: To investigate the relationship between human papillomavirus (HPV) infection and concurrent esophagus and gastric cardia cancer from the same patient (CC) and examine the significance of P16INK4A protein expression.
METHODS: Polymerase chain reaction was used to detect the presence of HPV type16 (HPV16). The expression of P16INK4A protein was detected using immunohistochemistry.
RESULTS: Among the CC specimens, HPV16-DNA was found in eight cases of esophageal squamous cell carcinoma (ESCC) and five cases of gastric cardia adenocarcinoma (GCA), respectively (47% vs 29%), and two of both ESCC and GCA. P16INK4A was highly expressed in both ESCC and GCA. In the HPV-associated positive CC, higher P16INK4A expression was observed in the GCA than in the ESCC (75% vs 25%, P < 0.05).
CONCLUSION: HPV16 as a correlated risk factor may play an important role in the development of ESCC and GCA. P16INK4A may be a screening index in the HPV-associated carcinoma of gastric cardia.
