Li H, Hou YX, Yang Y, He QQ, Gao TH, Zhao XF, Huo ZB, Chen SB, Liu DX. Tetramethylpyrazine inhibits proliferation of colon cancer cells in vitro. World J Clin Cases 2021; 9(18): 4542-4552 [PMID: 34222421 DOI: 10.12998/wjcc.v9.i18.4542]
Corresponding Author of This Article
Deng-Xiang Liu, PhD, Academic Research, Chief Doctor, Institute of Cancer Control, Xingtai People’s Hospital, No. 16 Hongxing Street, Xingtai 054001, Hebei Province, China. dengxianglfangliao@163.com
Research Domain of This Article
Gastroenterology & Hepatology
Article-Type of This Article
Basic Study
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Hua Li, Yan-Xu Hou, Qing-Qiang He, Tian-Hua Gao, Xiao-Feng Zhao, Zhi-Bin Huo, Shu-Bo Chen, Deng-Xiang Liu, Institute of Cancer Control, Xingtai People’s Hospital, Xingtai 054001, Hebei Province, China
Yu Yang, School of First Clinical Medicine, Nanjing University of Chinese Medicine, Nanjing 210029, Jiangsu Province, China
Author contributions: Li H, Hou YX and Yang Y contributed equally to this work; Chen SB and Liu DX designed the research; Li H and Yang Y analyzed the data and drafted the manuscript; Hou YX revised the manuscript critically for important intellectual content and contributed to data analysis; He QQ, Gao TH, Zhao XF, and Huo ZB helped draft the manuscript; all authors read and approved the final manuscript.
Institutional review board statement: Our study was not involving human and animal subjects.
Conflict-of-interest statement: The authors declare no conflict of interest related to this manuscript.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Deng-Xiang Liu, PhD, Academic Research, Chief Doctor, Institute of Cancer Control, Xingtai People’s Hospital, No. 16 Hongxing Street, Xingtai 054001, Hebei Province, China. dengxianglfangliao@163.com
Received: January 14, 2021 Peer-review started: January 14, 2021 First decision: March 15, 2021 Revised: March 27, 2021 Accepted: April 22, 2021 Article in press: April 22, 2021 Published online: June 26, 2021
ARTICLE HIGHLIGHTS
Research background
Colon cancer is one of the most common malignancies worldwide, and chemotherapy is a widely used strategy in clinical therapy for colon cancer. However, chemotherapy resistance is a major cause of recurrence and disease progression in colon cancer, and thus novel drugs for treatment are urgently needed. Tetramethylpyrazine (TMP), a component of the traditional Chinese medicine Chuanxiong Hort, has been proven to exhibit a beneficial effect in a number of types of malignant tumors.
Research motivation
To investigate the potential anticancer activity of TMP in colon cancer and its underlying mechanisms.
Research objectives
To investigate the potential anticancer activity of TMP in colon cancer and its underlying mechanisms.
Research methods
Colon cancer cells were incubated with different concentrations of TMP. Cell viability was evaluated by crystal violet staining assay and Cell Counting Kit-8 assay, and cell apoptosis and cell cycle were assessed by flow cytometry.
Research results
TMP significantly inhibited the proliferation of colon cancer cells in a dose- and time-dependent manner. In addition, flow cytometry revealed that TMP induced cell cycle arrest at the G0/G1 phase.
Research conclusions
Our study demonstrated that TMP inhibits the proliferation of colon cancer cells in a dose- and time-dependent manner by inducing apoptosis and arresting the cell cycle at the G0/G1 phase.
Research perspectives
Further studies of the mechanisms of TMP in human colon cancer are needed.
