Fan JZ, Wang GF, Cheng XB, Dong ZH, Chen X, Deng YJ, Song X. Relationship between mismatch repair protein, RAS, BRAF, PIK3CA gene expression and clinicopathological characteristics in elderly colorectal cancer patients. World J Clin Cases 2021; 9(11): 2458-2468 [PMID: 33889611 DOI: 10.12998/wjcc.v9.i11.2458]
Corresponding Author of This Article
Xin Song, MM, Associate Chief Technician, Department of Pathology, First Medical Center, Chinese People's Liberation Army General Hospital, No. 28 Fuxing Road, Haidian District, Beijing 100853, China. sxin66@hotmail.com
Research Domain of This Article
Pathology
Article-Type of This Article
Retrospective Study
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
World J Clin Cases. Apr 16, 2021; 9(11): 2458-2468 Published online Apr 16, 2021. doi: 10.12998/wjcc.v9.i11.2458
Relationship between mismatch repair protein, RAS, BRAF, PIK3CA gene expression and clinicopathological characteristics in elderly colorectal cancer patients
Jun-Zhen Fan, Gao-Fei Wang, Xue-Bin Cheng, Zhou-Huan Dong, Xin Chen, Xin Song, Department of Pathology, First Medical Center, Chinese People's Liberation Army General Hospital, Beijing 100853, China
Yu-Jiao Deng, Department of Ultrasound, First Medical Center, Chinese People's Liberation Army General Hospital, Beijing 100853, China
Author contributions: Fan JZ, Wang GF and Cheng XB made equal contributions to this article, they analyzed the data and drafted the paper; Dong ZH, Chen X and Deng YJ revised the paper; Song X analyzed the data, revised and finalized the paper.
Institutional review board statement: The study was reviewed and approved by the Ethics Committee of Chinese PLA General Hospital (Approval No. S2020-319-01).
Informed consent statement: Patients were not required to give informed consent to the study because the analysis used anonymous clinical data that were obtained after each patient agreed to treatment by written consent.
Conflict-of-interest statement: All authors declare no conflicts of interest related to this article.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Xin Song, MM, Associate Chief Technician, Department of Pathology, First Medical Center, Chinese People's Liberation Army General Hospital, No. 28 Fuxing Road, Haidian District, Beijing 100853, China. sxin66@hotmail.com
Received: December 22, 2020 Peer-review started: December 22, 2020 First decision: January 10, 2021 Revised: January 12, 2021 Accepted: February 1, 2021 Article in press: February 1, 2021 Published online: April 16, 2021
ARTICLE HIGHLIGHTS
Research background
Mismatch repair (MMR) protein deletion is one of the causes of colorectal cancer (CRC). The RAS (KRAS/NRAS), BRAF, and PIK3CA genes are important gene targets in CRC treatment and are closely related to the prognosis and survival of patients.
Research motivation
This study provides a further theoretical basis for clinicians in the diagnosis, treatment and prognosis of CRC.
Research objectives
This study aimed to explore the relationship between MMR, RAS (KRAS/NRAS), BRAF, PIK3CA and clinicopathological characteristics, and the relationship between MMR and the four target genes.
Research methods
The MMR protein was detected by immunohistochemistry, and real-time polymerase chain reaction was performed to detect KRAS, NRAS, BRAF, PIK3CA genes.
Research results
There were no significant differences between MMR protein deletion and sex, pathological type, tumor morphology, differentiation degree or lymph node metastasis, but there was a significant difference between MMR protein deletion and tumor diameter and tumor location. The KRAS gene mutation was closely related to tumor morphology, but not to other clinicopathological features, and there were no significant differences between NRAS gene mutation and clinicopathological features, MMR protein deletion and NRAS mutation.
Research conclusions
In elderly CRC patients, the deletion rate of MLH1 and PMS2 is more common; the mutation rate of KRAS gene is higher than that of the NRAS, BRAF and PIK3CA genes.
Research perspectives
The relationship between these indicators and the treatment or prognosis requires further investigation.
