Retrospective Study
Copyright ©The Author(s) 2025. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Psychiatry. Sep 19, 2025; 15(9): 109175
Published online Sep 19, 2025. doi: 10.5498/wjp.v15.i9.109175
Anxiety and depression in recurrent implantation failure after frozen-thawed embryo transfer and efficacy of endometrial receptivity testing
Ya-Bin Guo, Bin Tang, Ling Zhang, Xue Wu, Zhi-Hua Huang
Ya-Bin Guo, Bin Tang, Ling Zhang, Xue Wu, Center of Reproductive Medicine, Changde Hospital, Xiangya School of Medicine, Central South University (The First People’s Hospital of Changde City), Changde 415000, Hunan Province, China
Zhi-Hua Huang, Department of Neurology, Changde Hospital, Xiangya School of Medicine, Central South University (The First People’s Hospital of Changde City), Changde 415000, Hunan Province, China
Author contributions: Guo YB designed the study, analyzed the data, and performed the experiments; Guo YB, Tang B, Zhang L, and Wu X collected the data; Guo YB and Huang ZH prepared the manuscript; and all authors read and approved the final manuscript.
Institutional review board statement: This study was approved by the Medical Ethics Committee of the First People’s Hospital of Changde city.
Informed consent statement: Patients were not required to give informed consent to the study because the analysis used anonymous clinical data that were obtained after each patient agreed to treatment by written consent.
Conflict-of-interest statement: All the authors report no relevant conflicts of interest for this article.
Data sharing statement: No additional data are available.
Open Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Zhi-Hua Huang, Associate Chief Physician, Department of Neurology, Changde Hospital, Xiangya School of Medicine, Central South University (The First People’s Hospital of Changde City), No. 818 Renmin Road, Changde 415000, Hunan Province, China. hzhgyb@163.com
Received: May 14, 2025
Revised: June 22, 2025
Accepted: July 15, 2025
Published online: September 19, 2025
Processing time: 104 Days and 2 Hours
Abstract
BACKGROUND

Despite advances in the use of in vitro fertilization-embryo transfer (ET) in couples with infertility, recurrent implantation failure (RIF) after ET is still a major problem affecting women, with great psychological and economic burdens for the patient and his/her family.

AIM

To investigate the psychological burden (anxiety and depression) and evaluate the clinical benefits of endometrial receptivity testing (ERT) in patients experiencing RIF following frozen-thawed ET.

METHODS

A retrospective cohort study analyzed 371 patients experiencing RIF after assisted reproductive treatment at the First People’s Hospital of Changde City between January 2021 and June 2024. Demographic and clinical data were systematically collected through standardized questionnaires. Psychological assessment utilized validated instruments: The Self-Rating Depression Scale for depression evaluation and the Self-Rating Anxiety Scale for anxiety assessment. Participants were stratified by psychological status (anxiety/non-anxiety and depression/non-depression) to analyze influencing factors for anxiety and depression. The cohort was further categorized into the ERT and non-ERT groups based on ERT implementation to comparatively analyze their clinical outcomes. Additionally, they were divided into clinical and nonclinical pregnancy groups to identify factors affecting clinical pregnancy using univariate and multivariate logistic regression models. Compared with the non-ERT group, the 226 patients who underwent ERT-guided ET achieved a higher clinical pregnancy rate, thicker endometrium on transfer day, fewer embryos transferred, and a lower miscarriage rate.

RESULTS

The study identified a substantial psychological burden, with anxiety prevalence at 55.0% (mean Self-Rating Anxiety Scale score, 50.89 ± 9.34) and depression at 61.2% (mean Self-Rating Depression Scale score, 55.55 ± 9.48). Multivariate analysis identified annual household income > 100000 yuan as protective factors against both anxiety and depression, whereas advanced maternal age (> 35 years) and multiple implantation failures (≥ 3) served as risk factors. Additionally, anxiety-specific risk factors included prolonged infertility treatment (> 5 years) and the spouse’s status as an only child. As to depression-specific risks, chronic infertility (> 3 years) and higher educational attainment (college/bachelor’s degree or higher) were key determinants, whereas urban residence was a protective factor. Age > 35 years was a risk factor for clinical pregnancy in patients experiencing RIF, whereas blastocyst-stage ET, a higher number of embryos transferred, and thicker endometrium were protective factors.

CONCLUSION

Patients experiencing RIF are particularly susceptible to anxiety and depression, and advanced maternal age and multiple implantation failures represent salient risk factors. Clinicians should implement proactive and evidence-based interventions to mitigate these psychological burdens. For patients experiencing RIF, ERT-guided ET demonstrates significant potential to improve assisted reproductive outcomes.

Keywords: Recurrent implantation failure; Frozen-thawed embryo transfer; Psychological distress; Uterine receptivity assessment; Reproductive outcomes

Core Tip: Repeated implantation failures, i.e., three consecutive embryo transfer failures, occur in ≥ 20% of patients undergoing assisted conception, which not only imposes a huge economic burden on the patients but also brings about greater physical and psychological trauma. This study investigated the incidence of anxiety and depression in patients experiencing repeated implantation failures and analyzed factors influencing these conditions to propose a feasible and effective intervention model aimed at alleviating psychological distress and ultimately improving clinical pregnancy and birth rates.