Published online Sep 19, 2023. doi: 10.5498/wjp.v13.i9.620
Peer-review started: May 19, 2023
First decision: June 1, 2023
Revised: July 4, 2023
Accepted: July 28, 2023
Article in press: July 28, 2023
Published online: September 19, 2023
Autophagy is associated with hippocampal injury following status epilepticus (SE) and is considered a potential therapeutic mechanism. Baicalin, an emerging multitherapeutic drug, has shown neuroprotective effects in patients with nervous system diseases due to its antioxidant properties.
To investigate the potential role of autophagy in LiCl-pilocarpine-induced SE.
The drugs were administered 30 min before SE. Nissl staining showed that Baicalin attenuated hippocampal injury and reduced neuronal death in the hippocampus. Western blotting and terminal deoxynucleotidyl transferase dUTP nick end labeling assay confirmed that Baicalin reversed the expression intensity of cleaved caspase-3 and apoptosis in hippocampal CA1 following SE. Fur-thermore, western blotting and immunofluorescence staining were used to measure the expression of autophagy markers (p62/SQSTM1, Beclin 1, and LC3) and apoptotic pathway markers (cleaved caspase-3 and Bcl-2).
Baicalin significantly upregulated autophagic activity and downregulated mitochondrial apoptotic pathway markers. Conversely, 3-methyladenine, a commonly used autophagy inhibitor, was simultaneously administered to inhibit the Baicalin-induced autophagy, abrogating the protective effect of Baicalin on the mitochondrial apoptotic level.
We illustrated that Baicalin-induced activation of autophagy alleviates apoptotic death and protects the hippocampus of SE rats.
Core Tip: This study established the rat model of status epilepticus by intraperitoneally injecting LiCl-pilocarpine. Then, Baicalin was administered to the rats for treatment. The pathological changes of hippocampal were observed. Western blotting and terminal deoxynucleotidyl transferase dUTP nick end labeling assays were used to verify the inhibitory effect of Baicalin on apoptosis of rat hippocampal neuronal cells. We have drawn the conclusion that Baicalin protects the hippocampus from apoptosis.