Basic Study Open Access
Copyright ©The Author(s) 2019. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Exp Med. Dec 19, 2019; 9(2): 14-31
Published online Dec 19, 2019. doi: 10.5493/wjem.v9.i2.14
Localization of ATP-sensitive K+ channel subunits in rat liver
Ming Zhou, Hideo Akashi, Ryoji Suzuki, Yoshio Bando, Department of Anatomy, Akita University Graduate School of Medicine, Akita 010-8543, Japan
Kiwamu Yoshikawa, Mitsutaka Miura, Department of Cell Biology and Morphology, Akita University Graduate School of Medicine, Akita 010-8543, Japan
Tao-Sheng Li, Department of Stem Cell Biology, Atomic Bomb Disease Institute, Nagasaki University, Nagasaki 852-8523, Japan
Hiroshi Abe, TRUST, A Long-Term Care Health Facility, Sendai 980-0011, Japan
ORCID number: Ming Zhou (0000-0001-8775-9738); Kiwamu Yoshikawa (0000-0002-0053-6492); Hideo Akashi (0000-0001-8520-0252); Mitsutaka Miura (0000-0001-8454-8978); Ryoji Suzuki (0000-0003-4526-7406); Tao-Sheng Li (0000-0002-7653-8873); Hiroshi Abe (0000-0002-4683-4487); Yoshio Bando (0000-0002-2466-5248).
Author contributions: Zhou M did the main experiments, collected the data, and wrote the paper; Yoshikawa K and Miura M performed the experiment of primary culture for hepatic stellate cells and Kupffer cells; Akashi H and Suzuki R participated in treatment of animals; Li TS, Abe H, and Bando Y supervised the investigation and approved the manuscript for publication.
Supported by the Program of the network-type joint Usage/Research Center for Radiation Disaster Medical Science of Hiroshima University, Nagasaki University, and Fukushima Medical University.
Institutional review board statement: This study was approved by the Animal Research Committee of Akita University.
Institutional animal care and use committee statement: All procedures involving animals were reviewed and approved by the Animal Research Committee of Akita University (a-1-2405).
Conflict-of-interest statement: Authors declare no conflicts of interest for this article.
Data sharing statement: No additional data are available.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Corresponding author: Ming Zhou, MD, PhD, Assistant Professor, Department of Anatomy, Akita University Graduate School of Medicine, 1-1-1 Hondo, Akita 010-8543, Japan. mzhou@med.akita-u.ac.jp
Telephone: +81-18-8846260 Fax: +81-18-8846440
Received: May 17, 2019
Peer-review started: May 20, 2019
First decision: August 2, 2019
Revised: September 5, 2019
Accepted: November 20, 2019
Article in press: November 20, 2019
Published online: December 19, 2019
Processing time: 215 Days and 5.6 Hours

Abstract
BACKGROUND

ATP-sensitive K+ (KATP) channels were originally found in cardiac myocytes by Noma in 1983. KATP channels were formed by potassium ion-passing pore-forming subunits (Kir6.1, Kir6.2) and regulatory subunits SUR1, SU2A and SUR2B. A number of cells and tissues have been revealed to contain these channels including hepatocytes, but detailed localization of these subunits in different types of liver cells was still uncertain.

AIM

To investigate the expression of KATP channel subunits in rat liver and their localization in different cells of the liver.

METHODS

Rabbit anti-rat SUR1 peptide antibody was raised and purified by antigen immunoaffinity column chromatography. Four of Sprague-Dawley rats were used for liver protein extraction for immunoblot analysis, seven of them were used for immunohistochemistry both for the ABC method and immunofluorescence staining. Four of Wistar rats were used for the isolation of hepatic stellate cells (HSCs) and Kupffer cells for both primary culture and immunocytochemistry.

RESULTS

Immunoblot analysis showed that the five kinds of KATP channel subunits, i.e. Kir6.1, Kir6.2, SUR1, SUR2A, and SUR2B, were detected in liver. Immunohistochemical staining showed that Kir6.1 and Kir6.2 were weakly to moderately expressed in parenchymal cells and sinusoidal lining cells, while SUR1, SUR2A, and SUR2B were mainly localized to sinusoidal lining cells, such as HSCs, Kupffer cells, and sinusoidal endothelial cells. Immunoreactivity for SUR2A and SUR2B was expressed in the hepatocyte membrane. Double immunofluorescence staining further showed that the pore-forming subunits Kir6.1 and/or Kir6.2 colocalized with GFAP in rat liver sections and primary cultured HSCs. These KATP channel subunits also colocalized with CD68 in liver sections and primary cultured Kupffer cells. The SUR subunits colocalized with GFAP in liver sections and colocalized with CD68 both in liver sections and primary cultured Kupffer cells. In addition, five KATP channel subunits colocalized with SE-1 in sinusoidal endothelial cells.

CONCLUSION

Observations from the present study indicated that KATP channel subunits expressed in rat liver and the diversity of KATP channel subunit composition might form different types of KATP channels. This is applicable to hepatocytes, HSCs, various types of Kupffer cells and sinusoidal endothelial cells.

Key Words: ATP-sensitive K+ channel; Liver; Hepatic stellate cells; Kupffer cells; Sinusoidal endothelial cells; Rat

Core tip: ATP-sensitive K+ (KATP) channels have been found to be ubiquitously distributed in a variety of cell types and tissues, but the present study revealed that five kinds of KATP channel subunits are expressed in rat liver by applying western blot analysis, immunohistochemistry, and immunocytochemistry of rat liver tissue and primary cultured cells. These KATP channel subunits were not only localized in hepatocytes but also in sinusoidal cells, such as hepatic stellate cells, Kupffer cells, and sinusoidal endothelial cells. This was demonstrated using specific marker labelling, such as GFAP for hepatic stellate cells, CD68 for Kupffer cells, and SE-1 for sinusoidal endothelial cells. The wide diversity of KATP channel subunit compositions in rat liver might correspond to their various functions in hepatocytes and sinusoidal cells.



INTRODUCTION

ATP-sensitive K+ (KATP) channels were found ubiquitously distributed in a variety of cells and tissues[1], such as pancreatic β cells[2-5], skeletal muscle[6], neurons and glial cells[7-9], kidney[10-13], and liver[14,15]. KATP channels close at high concentrations of intracellular ATP and open at low concentrations during ischemia[7,16-18], and couple altered energy metabolism with membrane potential[19,20]. KATP channels are hetero-octamers composed of two kinds of subunits: the four pore-forming subunit Kir6.x (Kir6.1 or Kir6.2), and the four regulatory subunit sulfonylurea receptor SUR (SUR1, SUR2A, or SUR2B)[21-24]. Their physiological and pharmacological functions depend on their subunit compositions[25-27]. In the hypothalamus, glucose homeostasis is controlled by glucagon and catecholamine, which are counter-regulatory hormones, and these functions are involved with KATP channels[26,28]. The liver is also an organ that directly takes part in glucose metabolism with changes in intracellular ATP (ATPi) concentrations during which KATP channels are quite sensitive. The mRNA of KATP channels in liver is detected by northern blot analysis[14], KATP channels play roles in regulating hepatocyte proliferation in primary rat hepatocytes[29]. However, there are no detailed references on the localization of KATP channel molecules and their functions in the liver with respect to hepatocytes, hepatic stellate cells (HSCs), Kupffer cells, and sinusoidal endothelial cells (SECs). In addition to glucose metabolism, the liver has important functions in regeneration after injury and partial hepatectomy, as well as some functions of immune defense because of Kupffer cells. HSCs are localized between SECs and hepatocytes, and are activated during liver injury, producing many growth factors, chemokines, and cytokines, and also excessive extracellular matrix. They are also known as one of the reasons for hepatic fibrosis after liver injury recovery[30-33]. We are interested in clarifying the relationships between KATP channel subunits and hepatocytes, Kupffer cells, HSCs, and SECs in the liver during normal and pathological conditions.

Since there is no detailed information on the localization of KATP channel molecules in the liver with respect to different cells, we focused on the expression and localization of these KATP channel subunits in liver, and supply morphological data to help understand what functions they could play in parenchymal and non-parenchymal cells.

MATERIALS AND METHODS
Animals and tissue preparation

Sprague-Dawley and Wistar rats were supplied by CLEA Japan Inc. (Tokyo, Japan). This study was conducted according to the protocols of the Animal Research Committee of Akita University. We handled the animals according to the Guidelines of Akita University for Animal Experimentation. Rats used in this study were kept under constant environmental conditions in group cages (2-3 rats per cage) with 12 h light/dark cycles, and normal food and water were given.

Male SD rats (4-8 wk) under deep anesthesia were perfused through the left ventricle with cold Zamboni fixatives (2 g paraformaldehyde solved in 15 mL of saturated picric acid and Karasson Schwlt’s phosphate buffer solution added to make 100 mL). Liver tissue blocks were taken and further fixed in the same fixative for 6 h at 4 °C, and then changed to 30% sucrose in PBS until they sank to the bottom. No-fixed liver tissue was also taken for GFAP immunostaining. Cryosections were cut 10 µm thick with a Leica CM1950 cryostat, thaw-mounted on glass slides (MAS-coated, Matsunami Industries, Kishiwada, Japan) and stored at -20 °C before use. Liver samples for immunoblot analysis were directly taken out without fixation, put into dry ice powder, and stored at -80 °C until use.

Preparation of hepatic stellate cells and Kupffer cells

Male Wistar rats (400-500 g body mass), maintained on a normal chow diet, were used. Rats were perfused via the portal vein in situ with pre-perfusion solution (Ca2+/Mg2+-free Hanks’ balanced salt solution (HBSS) containing 10 mM hydroxyethyl piperazine ethanesulfonic acid (HEPES), 0.5 mM EGTA, 4.2 mM NaHCO3, and 5 mM glucose; pH 7.2) for 5 min at 37 °C, followed by collagenase solution (HBSS) containing 10 mM HEPES, 5 mM CaCl2, 4.2 mM NaHCO3, and 0.5 mg/mL bacterial collagenase; pH 7.5) for 15 min at 37 °C at a flow rate of 15 mL/min. Liver digested by collagenase solution was excised, dispersed, and filtered through stainless mesh. Isolation of HSCs and Kupffer cells was performed based on cell size and density[34]. To eliminate most of the parenchymal cells, the cell suspension was centrifuged at 50 × g for 1 min. The supernatant was further centrifuged at 50 × g for 3 min. The supernatant of the second run was centrifuged at 400 × g for 5 min to pellet non-parenchymal cells. The non-parenchymal cells in the pellet were further centrifuged at 700 × g for 30 min in 25% (v/v) Percoll PLUS solution (GE Healthcare Bio-Sciences, Piscataway, NJ, United States). HSCs were enriched in the supernatant, and Kupffer cells were mostly enriched in the pellet with other types of cells. The supernatant enriched in HSCs was diluted with 2 volumes of Dulbecco’s modified Eagle’s medium (DMEM) and centrifuged at 400 × g for 5 min to wash out Percoll PLUS. All centrifugations were performed at 4 °C. The cell fractions enriched in HSCs and Kupffer cells were suspended in culture media (DMEM containing 10% FBS, 100 U/mL penicillin, and 100 µg/mL streptomycin), and then plated on glass-bottom dishes coated with poly-L-lysine (Matsunami Glass, Osaka, Japan). Twelve to sixteen hours later, the cells were washed to remove debris, and then used for immunocytochemistry.

Generation of SUR1 antibody

Rabbit anti-rat SUR1 antibody was generated by immunization with a synthetic 22-mer peptide, NH2-DSALVLSPAARNCSLSQECALD-(COOH) (Biologica Co. Nagoya, Japan), which corresponds to amino acid residues of 1038-1059 of rat SUR1 (GenBank Accession No. L40624). Antiserum was purified by peptide antigen immunoaffinity column chromatography.

Western blot analysis

SDS/PAGE was performed as the procedure of Laemmli. Proteins were treated with modified sample buffer (125 mM Tris-HCl, pH 6.8, 2% SDS, 25% glycerol, 0.01% Bromophenol blue, and 10% 2-mercaptoethanol). After electrophoresis, proteins (10 µg/lane) were subsequently transferred to a polyvinylidene difluoride (PVDF) membrane (NENTM Life Science, Boston, MA, United States) using a semi-dry transfer unit (Hoefer TE70 series; Amersham Pharmacia Biotechnology, Little Chalfont, Buckinghamshire, England). After blocking with 5% BLOT-QuickBlocker (Chemicon International, Inc., Temecula, CA, United States) in PBS at room temperature overnight, immunoblotting was carried out by incubating the PVDF membranes with polyclonal goat anti-human Kir6.1 and/or Kir6.2 (Santa Cruz Biotechnology) and rabbit anti-rat SUR1, SUR2A and/or SUR2B antibody[35], each diluted to 1:1000, respectively, for 1 h at room temperature. Following thorough washing with PBS-T (PBS with 0.1% Tween-20), the PVDF membranes were then incubated with HRP-conjugated donkey anti-rabbit IgG (NA9340; Amersham Pharmacia Biotechnology, England), or HRP-conjugated donkey anti-goat IgG (AP180P), each diluted 1:3000. Signals were visualized with chemiluminescence detection reagents (Amersham Pharmacia Biotechnology, England), and PVDF membranes were exposed to XOMAT film (Eastman Kodak, Rochester, NY, United States) in the dark room. Negative controls and specificity verification of the SUR1 antibody were performed using the antigen peptide preabsorption test.

Immunohistochemistry

After air drying for 30 min, cryosections of liver were treated with 0.3% Tween-20 in PBS for 45 min at room temperature. Sections were blocked either with 5% normal rabbit serum or 5% normal goat serum for 30 min, and then incubated with goat anti-human Kir6.1 (Santa Cruz Biotechnology), goat anti-human Kir6.2 (Santa Cruz Biotechnology), and rabbit anti-rat SUR1, SUR2A, and/or SUR2B, each diluted at 1:500. After washing with PBS three times, the biotinylated rabbit anti-goat IgG, or biotinylated goat anti-rabbit IgG, were applied to sections. As a negative control, the immunoreactive solution was prepared without the primary antibody, and an absorption test was also confirmed by pre-incubation with peptide antigen.

To show whether the pore-forming subunits Kir6.1 and/or Kir6.2 are colocalized with the regulatory subunits SUR1, SUR2A and/or SUR2B in the liver, sections were incubated with a mixture of goat anti-human Kir6.1 and rabbit anti-rat SUR1, and/or anti-rat SUR2A, and/or SUR2B; or a mixture of goat anti-human Kir6.2 with rabbit anti-rat SUR1, SUR2A and/or SUR2B antibody, each diluted 1:500, respectively, for 12 h at room temperature. Sections were then incubated with a mixture of Alexa488-conjugated donkey anti-goat IgG (A21432; Molecular Probes, Inc., Eugene, OR) and Alexa594-conjugated donkey anti-rabbit IgG (A21207; Molecular Probes, Inc., Eugene, OR), each diluted 1:200.

To determine whether the KATP channel subunit molecules colocalize with HSCs, Kupffer cells and/or SECs, sections were incubated with a mixture of mouse anti-GFAP (marker of HSCs), or incubated with a mixture of mouse anti-CD68 (marker of Kupffer cells), and/or incubated with a mixture of mouse anti-SE-1 antibody (marker of SECs) and one of the KATP channel subunit antibodies, such as goat anti-human Kir6.1, goat anti-human Kir6.2, rabbit anti-rat SUR1, rabbit anti-rat SUR2A, and/or rabbit anti-rat SUR2B, respectively, each diluted 1:200. After thoroughly washing with PBS, the liver sections were then incubated for 30 min with a mixture of the appropriate fluorescent Alexa 488 or Alexa 594-conjugated secondary antibodies (Molecular Probes, Inc., Eugene, OR) each diluted 1:200. Finally, the sections stained above were mounted with aqueous mounting medium (Thermo, Pittsburgh, PA, United States).

Primary cultured HSCs and Kupffer cells were fixed with Zamboni’s fixatives for 5 min and then double immunofluorecent staining was carried out as (1) mouse anti-GFAP with one of the antibodies of the pore-forming subunits Kir6.1 and/or Kir6.2, and/or one of the antibodies of the regulatory subunits SUR1, SUR2A and/or SUR2B; (2) mouse anti-CD68 with one of the antibodies of goat anti-human Kir6.1, and/or Kir6.2, and rabbit anti-rat SUR1, SUR2A and/or SUR2B; (3) goat anti-human Kir6.1 or Kir6.2 with one of the antibodies of rabbit anti-rat SUR1, SUR2A, and/or SUR2B. After thoroughly washing with PBS, the dishes were incubated for 30 min with the appropriate fluorescent Alexa 488- or Alexa 594-conjugated secondary antibodies (Molecular Probes, Inc., Eugene, OR) each diluted 1:200. Fluorescence image signals were acquired using an Olympus microscope (BX51; Tokyo, Japan) and a laser scanning confocal microscope (LSM 510; Carl Zeiss, Germany).

RESULTS
Expression of KATP channel subunits in rat liver

Immunoblot analysis demonstrated that Kir6.1, Kir6.2, SUR2A and SUR2B were expressed in rat liver (Figure 1A). For the anti-SUR1 antibody, immunoblot analysis showed that the molecular weight of SUR1 was about 100 kDa with some weak small bands below. After preincubation with immunizing peptide from which the antibody was raised, the SUR1 band disappeared (Figure 1B, +peptide).

Figure 1
Figure 1 The expression of ATP-sensitive K+ channel subunits in rat liver extracts. A: Antibodies to Kir6.1, Kir6.2, SUR2A and/or SUR2B showed prominent specific bands, respectively; B: A prominent band around 100 kDa was recognized with our new anti-SUR1 antibody. The band disappeared when blocked by preincubation with the corresponding SUR1 peptide antigen from which the antibody had been raised (+peptide).

Immunohistochemistry revealed that immunoreactivity for Kir6.1 was widely and weakly observed in hepatocytes in the hepatic lobules (Figure 2A), as well as in the endothelia of the central vein, the branches of the hepatic artery, and the portal vein at the portal area (Figure 2C). The intensity of immunoreactivity was gradually decreased along the liver plate from the central vein to the distal area (Figure 2A). Weak immunoreactivity for Kir6.1 was expressed in the cell membrane and cytoplasm of hepatocytes. Under high magnification, the Kir6.1 protein was observed as punctate immunoreactive products in the cytoplasm of hepatocytes and sinusoidal lining cells (Figure 2B). When the anti-Kir6.1 antibody was omitted or preincubated with antigen peptide, significant immunoreactivity could not be observed (Figure 2D).

Figure 2
Figure 2 Kir6. 1 immunoreactivity in liver sections. Kir6.1 was expressed in hepatocytes and sinusoidal cells of the liver. A: Staining was intense in the area around the central vein; B: Showing punctate reactivity around the nucleus in hepatocytes cells and sinusoidal cells when viewing with high magnification; C: Weaker immunoreactivity was evident in the triangular areas of the vein and artery, but no reactivity was evident in the bile duct; D: A negative control section devoid of staining. a: Artery; Bd: Bile duct, Cv: Central vein; Pv: Portal vein. Bars: 100 µm (A and C), 20 µm (B), 50 µm (D).

Immunoreactivity for Kir6.2 was also weakly distributed in the hepatic lobule (Figure 3A) and in the endothelium of the central vein, the branches of the hepatic artery, and the portal vein in the portal area (Figure 3C). The intensity of immunoreactivity was gradually decreased along the liver plate (Figure 3A and B). Weak immunoreactivity for Kir6.2 was expressed in the cell membrane and cytoplasm of hepatocytes. Under high magnification, the immunoreactive products for Kir6.2 could be observed as rough granules in the cytoplasm of hepatocytes and sinusoidal lining cells (Figure 3B). When the anti-Kir6.2 antibody was omitted or pre-incubated with immunizing antigen peptides, no significant immunoreactivity could be observed (Figure 3D).

Figure 3
Figure 3 Immunoreactivity with Kir6. 2 was expressed in hepatocytes and sinusoidal cells of the liver. A and B: Mediated immunoreactivity was seen in the area around the central vein; A and C: Mediated immunoreactivity was weaker in the border areas; D: A negative control section devoid of staining (no more than background staining was observed). a: Artery; Bd: Bile duct, Cv: Central vein; Pv: Portal vein. Bars: 100 µm (A and C), 20 µm (B), 50 µm (D).

Immunoreactivity for SUR1 was quite different from that for Kir6.1 and Kir6.2. Moderate immunoreactivity for SUR1 was distributed in small sinusoidal lining cells with round, ovular, or spindle shapes beside the liver plate (Figure 4A and B), and also in the endothelial cells of the branches of hepatic artery and portal veins at the portal area (Figure 4C). The intensity was not decreased along the liver plate. In hepatocytes, the immunoreactivity for SUR1 was hardly observed in the cytoplasm, and only faintly in the cell membrane (Figure 4B). When the anti-SUR1 antibody was omitted or preincubated with immunizing peptide antigen, the positive immunoreactivity disappeared (Figure 4D).

Figure 4
Figure 4 Immunoreactivity with SUR1 was mainly evident in sinusoidal cells (arrows) and weaker in the cell membranes of hepatocytes. A and B: There was no such tendency toward immunoreactivity with SUR1 in the area of the central vein vs the border area; C: In the portal area, endothelial cells of the blood vessels showed intense immunoreactivity with SUR1; D: Absorption negative control section devoid of staining (no more than background) after incubation with cognate antigen peptide. a: Artery, Cv: Central vein; Pv: Portal vein. Bars: 100 µm (A and C), 20 µm (B), 50 µm (D).

Immunoreactivity for SUR2A was distributed in the hepatic lobule with moderate intensity around the central vein (Figure 5A), and gradually attenuated along the liver plate. It was mainly localized in irregular- or spindle-shaped cells in the sinusoidal lining (Figure 5B). Weak immunoreactivity for SUR2A was expressed in the cell membrane of hepatocytes. In the portal area, it was weakly expressed in the branches of hepatic arteries and portal veins (Figure 5C).

Figure 5
Figure 5 Immunoreactivity with SUR2A was evident in hepatocytes and sinusoidal cells (arrows) of the liver. A: Immunoreactivity with SUR2A was stronger in the area of the central vein (Cv) vs the border areas; B: Weaker immunoreactivity in the cell membrane and moderate immunoreactivity in the sinusoidal cells were observed (arrows); C: Weaker immunoreactivity with SUR2A was also observed in the endothelial cells of the blood vessels; D: Absorption negative control section devoid of staining (no more than background was observed). a: Artery. Cv: Central vein; Pv: Portal vein. Bars: 100 µm (A and C), 20 µm (B), 50 µm (D).

Immunoreactivity for SUR2B was widely and weakly distributed in the hepatic lobule (Figure 6A). It was mainly localized in small round or oval-shaped cells in the sinusoidal lining. Weak immunoreactivity for SUR2B was expressed in the cell membrane of hepatocytes (Figure 6B). The intensity of immunoreactivity was gradually decreased along the liver plate from the central vein to the distal area (Figure 6A). In the portal area, it was moderately expressed in the branches of the hepatic artery and portal vein, and was especially intense in the bile duct (Figure 6C). When the anti-SUR2A or anti-SUR2B antibodies were omitted or preincubated with each immunizing peptide antigen, the immunoreactivity for SUR2A and/or SUR2B disappeared (Figures 5D and 6D).

Figure 6
Figure 6 Immunoreactivity with SUR2B. A: Immunoreactivity with SUR2B was stronger in the area of the central vein (Cv) vs the border areas; B: Immunoreactivity with SUR2B was expressed in hepatocytes and sinusoidal cells (arrows) of the liver. Weaker immunoreactivity in the cell membrane and medium immunoreactivity in sinusoidal cells was observed (arrows); C: Immunoreactivity with SUR2B was also observed in the endothelial cells of blood vessels and bile ducts; D: Absorption negative control section devoid of staining (no more than background was observed). Bd: Bile duct; Cv: Central vein; Pv: Portal vein. Bars: 100 µm (A), 20 µm (B), 50 µm (C and D).
Colocalization among KATP channel subunits and sinusoidal cell marker proteins

To show if pore-forming subunits are co-localized with regulatory subunits in rat liver, immunofluorescence double staining was performed. Positive immunoreactivity of Kir6.1 (Kir6.1+) and Kir6.2 (Kir6.2+) was detected as green fluorescence (Alexa488, Figures 7A, 7D, 8A, 8D, 9A and 9D), and that of SUR1 (SUR1+), SUR2A (SUR2A+) and SUR2B (SUR2B+) was detected as red fluorescence (Alexa594, Figures 7B, 7E, 8B, 8E, 9B, and 9E). In hepatocytes, Kir6.1+ and/or Kir6.2+ were hardly observed to be colocalized with SUR1+, SUR2A+, and/or SUR2B+. Instead, in a small number of round, oval, or spindle-shaped cells located beside the sinusoid, Kir6.1+ and/or Kir6.2+ cells were colocalized with that for SUR1, SUR2A, and/or SUR2B (Figures 7C, 7F, 8C, 8F, 9C, and 9F). When labeled by GFAP, a marker of HSCs, some of the Kir6.1+ and/or Kir6.2+ cells were colocalized with GFAP beside the sinusoid (Figure 10). GFAP+ cells also showed immunoreactivity to SUR1, SUR2A, and SUR2B in the sinusoidal lining (Figure 10). When labeled with CD68, a marker of Kupffer cells, a few CD68+ cells were colocalized with Kir6.1 and Kir6.2, and more of these small CD68+ cells were showed immunoreactivity to SUR1, SUR2A and SUR2B (Figure 11).

Figure 7
Figure 7 Immunofluorescence double staining for Kir6. 1 and/or Kir6.2 with SUR1 in liver sections. A and D: Kir6.1 and Kir6.2 were widely localized in hepatocyte and sinusoidal cells; B and E: SUR1 was mainly localized in sinusoidal cells; C: There were few sinusoidal cells that had colocalized Kir6.1 and SUR1 (arrows); F: There were few sinusoidal cells that had colocalized Kir6.2 and SUR1 (arrows). Bars: 20 µm.
Figure 8
Figure 8 Immunofluorescence double staining for Kir6. 1 and/or Kir6.2 with SUR2A in liver sections. A and D: Kir6.1 and Kir6.2 were widely localized in hepatocyte and sinusoidal cells; B and E: SUR2A was mainly localized in sinusoidal cells; C: There were few sinusoid cells with colocalized Kir6.1 and SUR2A (arrows); F: There were few sinusoidal cells with colocalized Kir6.2 and SUR2A (arrows). Bars: 20 µm.
Figure 9
Figure 9 Immunofluorescence double staining for Kir6. 1 and/or Kir6.2 with SUR2B in liver sections. A and D: Kir6.1 and Kir6.2 were widely localized in hepatocyte and sinusoidal cells; B and E: SUR2B staining was mainly present in sinusoidal cells; C: There were few sinusoidal cells with colocalized Kir6.1 and SUR2B (arrows); F: There were few sinusoid cells with colocalized Kir6.2 and SUR2B (arrows). Bars: 20 µm.
Figure 10
Figure 10 Immunofluorescence double staining. Immunofluorescence double staining for Kir6.1, Kir6.2, SUR1, SUR2A, and/or SUR2B (A, D, G, J, and M) and GFAP [B, E, H, K, and N, marker of hepatic stellate cells (HSCs)] in no-fixed liver sections; Most of the GFAP+ cells (HSCs) colocalized with Kir6.1 (C, arrows) and/or Kir6.2 (F, arrows); There were few GFAP+ cells (HSCs) colocalized with SUR1 (i, arrows), SUR2A (L, arrows), and/or SUR2B (O, arrows). Bars: 20 µm.
Figure 11
Figure 11 Immunofluorescence double staining for ATP-sensitive K+ channel subunits Kir6. 1, Kir6.2, SUR1, SUR2A and SUR2B with CD68 in liver sections. Immunofluorescence double staining for ATP-sensitive K+ channel subunits Kir6.1 (A), Kir6.2 (D), SUR1 (G), SUR2A (J) and SUR2B (M) with CD68 (B, E, H, K, and N, marker of Kupffer cells) in liver sections; Some CD68+ cells (Kupffer cells) colocalized with Kir6.1 (C, arrows) and/or Kir6.2 (F, arrows), but most colocalized with SUR1 (I, arrows), SUR2A (L, arrows) and SUR2B (O, arrows). Bars: 20 µm.

Similarly, when labeled with SE-1, a marker of SECs, a few SE-1+ cells colocalized with Kir6.1, SUR1, and SUR2B. On the other hand, a number of SE-1+ cells colocalized with Kir6.2 and SUR2A, respectively (Figure 12).

Figure 12
Figure 12 Immunofluorescence double staining for ATP-sensitive K+ channel subunits Kir6. 1, Kir6.2, SUR1, SUR2A and SUR2B with SE-1. Immunofluorescence double staining for ATP-sensitive K+ channel subunits Kir6.1, Kir6.2, SUR1, SUR2A and SUR2B (A, D, G, J, and M) with SE-1 [marker of sinusoidal endothelial cells (SECs)]; In the liver sections, SECs showed immunopositive reactivity to SE-1 (B, E, H, K, and N); A few SE-1+ cells colocalized with Kir6.1 (C, arrows), but a number of them colocalized with Kir6.2 (F, arrows); As for SURs, some SE-1+ cells colocalized with SUR1 (I, arrows) and/or SUR2B (O, arrows); Most SE-1+ cells colocalized with SUR2A (L, arrows). Bars: 20 µm.

Primary HSCs were labeled with GFAP antibody. GFAP+ cells also showed positive immunoreactivity for Kir6.1 and/or Kir6.2 (Figure 13). This result indicated that the pore-forming subunits of the KATP channel were expressed in HSCs. In these HSCs, immunoreactivity for Kir6.1 and Kir6.2 were located in the peripheral cytoplasm, and that for GFAP was located in the perinuclear region. Colocalization of SURs and GFAP in primary HSCs has not been observed.

Figure 13
Figure 13 Immunofluorescence double staining for pore-forming subunits in primary cultured hepatic stellate cells. The primary hepatic stellate cells were marked by GFAP (red), they showed immunoreactivity to Kir6.1 and Kir6.2 (green). Bars: 50 µm.

Primary Kupffer cells showed immunoreactivity for CD68, which confirmed them to be Kupffer cells. When incubating these cells with antibodies of KATP channel subunits, the CD68+ cells showed immunopositive reactivity for Kir6.1, Kir6.2, SUR1, SUR2A, and SUR2B (Figure 14).

Figure 14
Figure 14 Immunofluorescence double staining for ATP-sensitive K+ channel subunits in primary cultured Kupffer cells. Primary Kupffer cells were labeled with CD68 (red). These CD68+ Kupffer cells also showed immunoreactivity to five kinds of ATP-sensitive K+ channel subunits: Kir6.1, Kir6.2, SUR1, SUR2A and SUR2B (green). Bars: 50 µm.

Furthermore, when primary Kupffer cells were incubated with multiple paired combined antibodies of pore-forming subunits and regulatory subunits, they showed immunoreactivity for Kir6.1/SUR1, Kir6.1/SUR2A, Kir6.1/SUR2B, Kir6.2/SUR1, Kir6.2/SUR2A, and Kir6.2/SUR2B (Figure 15). The immunoreactive products for KATP channel subunits exhibited a punctate or granular appearance.

Figure 15
Figure 15 Immunofluorescence double staining for combinations of pore-forming subunits and regulatory subunits in primary cultured Kupffer cells. In primary Kupffer cells, the pore-forming subunits Kir6.1 and Kir6.2 are labeled in green, and the regulatory subunits SUR1, SUR2A and SUR2B are labeled in red. The combinations of Kir6.1/SUR1, Kir6.1/SUR2A, Kir6.1/SUR2B, Kir6.2/SUR1, Kir6.2/SUR2A and Kir6.2/SUR2B are all exhibited in the Kupffer cells. Bars: 50 µm.
DISCUSSION

In this study, a new polyclonal anti-SUR1 antibody was generated and applied to investigate the exhibition and localization of SUR1 in rat liver. Its specificity was assessed by preabsorption with an immunizing peptide antigen in western blot analysis (Figure 1B). The most significant band at 100 kDa disappeared in the presence of the immunizing antigen, showing the high specificity of the antiserum. The specificity of the antisera for SUR2A and SUR2B was demonstrated elsewhere[35]. The expression of five kinds of KATP channel subunits in rat liver extract was revealed by western blot analysis with the antibodies, including this new anti-SUR1, and every antibody revealed their corresponding band in the liver extract, respectively (Figure 1). This means that the rat liver contains five kinds of KATP channel subunits: Kir6.1, Kir6.2, SUR1, SUR2A, and SUR2B.

In liver sections, immunoreactivity for Kir6.1, Kir6.2, SUR2A and SUR2B was weakly to moderately expressed around the central vein and gradually decreased along the liver plate (Figures 2, 3, 5, and 6), but the immunoreactivity for SUR1 showed no such tendency (Figure 4). The Kir6.1 and Kir6.2 pore-forming subunits of the KATP channel were weakly expressed in hepatocytes and sinusoidal lining cells, such as HSCs, Kupffer cells, and SECs. Under high magnification, the Kir6.1 protein was detected as punctate immunoreactive products (Figure 2B) and the Kir6.2 protein was detected as granular immunoreactive products, indicating that Kir6.1 was located in the mitochondria and Kir6.2 in the endoplasmic reticulum, as shown in previous studies on rat brain[8,9]. The regulatory subunits SUR1, SUR2A, and SUR2B were mainly located in small cells, and exhibited staining in sinusoidal cells and weak staining in the cell membrane of hepatocytes.

The KATP channels play important roles in hyperglycemia, hypoglycemia, ischemia and hypoxia[16,36]. Kir6.1 mRNA and protein are upregulated during myocardial infarction[17,37,38]. When the liver is in ischemia followed by reperfusion, hepatocytes around the central veins were more markedly degenerated than those in the peripheral zone of the liver lobule[38]. During liver ischemia, the different degenerated zonal areas are accordant with the liver blood supply being made up of different gradients of oxygen, hormones, and metabolites, as well as zonal differences form periportal to perivenous[39,40]. The similar zonal differences in immunoreactivity for Kir6.1, Kir6.2, SUR2A, and SUR2B might indicate that these subunits of KATP channels have some relationship with the protection of hepatocytes, though colocalization of these subunits was hardly observed in the sections of normal rat liver. KATP channels are important in liver regeneration for keeping a higher ATP content in liver tissue during partial hepatectomy[15] since, clinically, hepatocyte regeneration is a key point for partial liver transplantation.

It is well known that, in addition to parenchymal cells, the liver consists of three other kinds of cells, i.e. HSCs, Kupffer cells, and SECs[41]. HSCs are located between hepatocytes and SECs[31]. They store large amounts of vitamin A and produce extracellular matrix for liver fibrogenesis[31,41]. The pore-forming subunits (Kir6.1 and Kir6.2) were clearly colocalized in HSCs with GFAP both in liver sections and primary cells (Figures 10 and 13). Although the regulatory subunits (SURs) were not yet observed in primary HSCs, they were colocalized in HSCs with GFAP in liver sections (Figure 11). These results might indicate that the KATP channels have some relation to HSC matrix secretion and fibrogenesis.

In addition to the metabolism of glucose, lipids, hormones, and various substances, the liver also plays important roles in the immune response to eliminate pathogens, such as microorganisms, endotoxins, portal antigens, and tumor cells, and Kupffer cells are responsible for these crucial functions of host defense[42]. There are several subsets of Kupffer cells categorized by the cytokines they produce, such as CD11b+, CD68+, CD11b- and/or CD68-, CD32, and so on[40,43]. Kupffer cells can also be divided into two groups: bone marrow-derived macrophages and sessile hepatic macrophages. These different groups of Kupffer cells play different roles in the liver. For instance, when mice were given food with high-fat or high-cholesterol, CD68+ Kupffer cells decreased while CD11b+ Kupffer cells increased in number[40]. KATP channels exhibit different physiological and pharmacological functions depending on their subunit composition[26]. For example, Kir6.2 with SUR1 is strongly sensitive to the KATP channel activator diazoxide, but not to other activators such as pinacidil[25], while Kir6.2 with SUR2A is strongly sensitive to pinacidil and cromakalin but only weakly to diazoxide[26]. The diversity of subunit composition of KATP channels in Kupffer cells might correspond to different types of Kupffer cells or to their various functional stages.

In SECs, SE-1 immunopositive cells were colocalized in different numbers with the five kinds of KATP channel subunits, respectively (Figure 12). It is well known that SECs are highly specialized endothelial cells (with different classifying types) located between blood, hepatocytes and HSCs[31]. In the physiological state, SECs have a low proliferation rate. They maintain HSC quiescence, thus inhibiting intrahepatic vasoconstriction and fibrosis development under normal conditions. They are also differentially renewable under physiological and pathological conditions[44]. They are also involved in liver regeneration following acute liver injury and partial hepatectomy[45]. They play a key role in promoting liver regeneration by orchestrating the harmonious regeneration of the different cell types and renew themselves with asynchronous liver regeneration[44]. In the present study, SECs showed different combinations of Kir6.x and SURs, which indicates that they have multiple kinds of KATP channels corresponding with their multiple functions under physiological and pathological conditions.

The present study is, to our knowledge, the first report that HSCs, Kupffer cells, and SECs contain KATP channel subunits. Further investigation is needed to elucidate their functions in detail in liver metabolism, immunity, and fibrogenesis during liver injury.

ARTICLE HIGHLIGHTS
Research background

ATP-sensitive K+ (KATP) channels coupling nutrient metabolism to membrane potential are sensitive to intracellular ATP concentrations and ATP/ADP ratios. KATP channels are hetero-octameric in composition, formed by four pore-forming subunits including Kir6.1 and Kir6.2, which belong to the inward rectifying potassium channel family, and four regulatory subunits, SUR1, SUR2A and SUR2B, which belong to members of the ATP-binding cassette protein family. KATP channels were considered to be involved in cellular functions, such as pancreatic β cell secretion, muscle and neuronal excitability, and so on.

Research motivation

How KATP channels work in liver hepatocytes and sinusoidal cells is important to help understand liver function under normal and injury conditions. The first step is to characterize the localization and distribution of KATP channel subunits in different cells of the liver. This research focused on the localization and distribution of KATP channel subunits in hepatocytes and sinusoidal cells of rat liver. This research will provide the basic data to guide further research in medicine exploitation and clinical treatment for related liver diseases.

Research objectives

To investigate the expression of KATP channel subunits in rat liver and their localization in different cells of the liver.

Research methods

In order to obtain basic knowledge of KATP channel subunits in rat liver distribution and localization, extracted liver protein was used for immunoblot analysis, cryosections of liver were used for immunohistochemistry, and primary cultured hepatic stellate cells (HSCs) and Kupffer cells were used for immunocytochemistry.

Research results

In the present study, five types of KATP channel subunits, including Kir6.1, Kir6.2, SUR1, SUR2A and SUR2B, were expressed in rat liver as revealed by immunoblot analysis and immunohistochemistry. Double immunofluorescence staining of cryosections, primary cultured HSCs and Kupffer cells showed that not only hepatocytes, but also HSCs, Kupffer cells and sinusoidal endothelial cells (SECs) contain KATP channel subunits.

Research conclusions

The present study revealed that KATP channel subunits are expressed in rat liver, which is the first report that HSCs, Kupffer cells, and SECs contain KATP channel subunits formed by Kir6.1/SUR1, Kir6.1/SUR2A, Kir6.1/SUR2B, Kir6.2/SUR1, Kir6.2/SUR2A, and Kir6.2/SUR2B. Different combinations of KATP channels have different physiological and pharmacological characteristics. Thus, this research will supply basic experimental data to guide further exploration of liver function under normal and injury conditions.

Research perspectives

In the present study, all experiments were performed under normal conditions. Thus, further study is needed to elucidate changes in KATP channel subunit composition under different metabolic states, including ischemia and hypoxia in vitro and/or in vivo with the use of KATP channel openers and blockers, such as diazoxide, pinacidil, glibenclamide, and tolbutamide.

Footnotes

Manuscript source: Invited manuscript

Specialty type: Medicine, research and experimental

Country of origin: Japan

Peer-review report classification

Grade A (Excellent): 0

Grade B (Very good): 0

Grade C (Good): C

Grade D (Fair): 0

Grade E (Poor): 0

P-Reviewer: Gencdal G S-Editor: Yan JP L-Editor: Filipodia E-Editor: Ma YJ

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