Retrospective Study
Copyright ©The Author(s) 2016. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastrointest Pharmacol Ther. May 6, 2016; 7(2): 254-260
Published online May 6, 2016. doi: 10.4292/wjgpt.v7.i2.254
Clinical utility of quantitative multi-antibody Polycheck immunoassays in the diagnosis of coeliac disease
Ewa Konopka, Maciej Grzywnowicz, Beata Oralewska, Joanna Cielecka-Kuszyk, Ilona Trojanowska, Bożena Cukrowska
Ewa Konopka, Joanna Cielecka-Kuszyk, Ilona Trojanowska, Bożena Cukrowska, Department of Pathology, the Children’s Memorial Health Institute, 04-730 Warsaw, Poland
Maciej Grzywnowicz, Department of Experimental Hematooncology, Medical University of Lublin, 20-059 Lublin, Poland
Beata Oralewska, Department of Gastroenerology, Hepatology, Nutritional Disorders and Pediatrics, the Children’s Memorial Health Institute, 04-730 Warsaw, Poland
Author contributions: Konopka E and Grzywnowicz M contributed equally; all authors contributed to this manuscript.
Supported by S135/2013 and 229/14 grants from the Children’s Memorial Health Institute.
Institutional review board statement: The study was reviewed and approved by the Children’s Memorial Health Institute Bioethical Committee Review Board.
Informed consent statement: All patient involved in the study obtained informed consent statement from the principal investigator.
Conflict-of-interest statement: Grzywnowicz M is an employee of the EMMA Paweł Majsiak company. For the remaining authors, none are declared.
Data sharing statement: Technical appendix, statistical code, and dataset available from the corresponding author at b.cukrowska@czd.pl. Informed consent for data sharing was not obtained. The presented data are anonymized and there is no risk identification.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Bożena Cukrowska, Professor, Department of Pathology, the Children’s Memorial Health Institute, Aleja Dzieci Polskich 20, 04-730 Warsaw, Poland. b.cukrowska@czd.pl
Telephone: +48-228-151091 Fax: +48-228-15197
Received: April 10, 2015
Peer-review started: April 11, 2015
First decision: August 1, 2015
Revised: October 13, 2015
Accepted: January 21, 2016
Article in press: January 22, 2016
Published online: May 6, 2016
Core Tip

Core tip: Detection of coeliac-specific antibodies has become a useful tool in the diagnostics of coeliac disease. Different serology test combinations have been found to improve diagnosis in comparison to a single antibody test. Recently, multi-antibody strategy has been implemented in immunoassays. In this study we have found that multi-parametric quantitative Polycheck immunoassay is reliable in reference to intestinal biopsy results and measurements of anti-tissue transglutaminase-IgA by a reference method. The best overall clinical performance was obtained by a combination of both IgA and IgG panels, with two and more positively detected antibodies, to determine the outcome.