Published online May 15, 2020. doi: 10.4251/wjgo.v12.i5.526
Peer-review started: January 20, 2020
First decision: March 24, 2020
Revised: March 29, 2020
Accepted: April 15, 2020
Article in press: April 15, 2020
Published online: May 15, 2020
The integrin β6 gene, which is expressed in epithelial cancer, plays a pivotal role in various aspects of cancer progression. The present research for integrin β6 regulation mainly focuses on the post-transcription and translation related regulation mechanism and its role in tumorigenesis.
The mechanism of how the integrin β6 gene is regulated, and the promoter and transcription factors responsible for basic transcription of integrin β6 gene remain unknown.
This study aimed to clone and characterize the integrin β6 promoter.
The region containing the integrin β6 promoter was predicted and luciferase reporter plasmids were constructed. The location of core promoter and binding site for transcription factors were identified by element deletion analysis.
We found that the regulatory elements for transcription of integrin β6 gene were located between -286 and-85 and contained binding sites for transcription factors such as STAT3 and Ets-1.
We have identified the region of β6 core promoter and the binding sites for transcription factors such as Ets-1 and STAT3, which are important for integrin β6 promoter transcription activity.
The findings of this study are important for investigating the mechanism of integrin β6 activation in cancer progression.