Basic Study
Copyright ©The Author(s) 2020. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastrointest Oncol. May 15, 2020; 12(5): 526-534
Published online May 15, 2020. doi: 10.4251/wjgo.v12.i5.526
Identification of integrin β6 gene promoter and analysis of its transcription regulation in colon cancer cells
Wei Niu, Qi-Yu Bo, Jun Niu, Zheng-Chuan Niu, Cheng Peng, Xue-Qing Zou, Zhao-Yang Zhang
Wei Niu, Jun Niu, Zheng-Chuan Niu, Cheng Peng, Xue-Qing Zou, Department of Hepatobiliary Surgery, Qilu Hospital, Shandong University, Jinan 250012, Shandong Province, China
Qi-Yu Bo, Department of Nursing, Qilu Hospital, Shandong University, Jinan 250012, Shandong Province, China
Zhao-Yang Zhang, Department of Emergency Surgery, Qilu Hospital, Shandong University, Jinan 250012, Shandong Province, China
Author contributions: Niu W and Bo QY contributed equally to this article and should be considered as co-first authors; Niu W and Bo QY designed the experiments; Zhang ZY drafted the work and collected the data; Niu J, Niu ZC, Peng C, Zou XQ, and Zhang ZY analysed and interpreted the data and wrote the article.
Supported by National Sciences Foundation of Shandong Province, No. ZR2014HM101.
Institutional review board statement: The study was reviewed and approved by the Institutional Review Board of Qilu Hospital, Shandong University.
Conflict-of-interest statement: The authors declare no conflicts of interest related to this manuscript.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Corresponding author: Zhao-Yang Zhang, MD, Associate Chief Physician, Associate Professor, Department of Emergency Surgery, Qilu Hospital, Shandong University, No. 44, Wenhua Xi Road, Jinan 250012, Shandong Province, China.drzzyang@163.com
Received: January 20, 2020
Peer-review started: January 20, 2020
First decision: March 24, 2020
Revised: March 29, 2020
Accepted: April 15, 2020
Article in press: April 15, 2020
Published online: May 15, 2020
ARTICLE HIGHLIGHTS
Research background

The integrin β6 gene, which is expressed in epithelial cancer, plays a pivotal role in various aspects of cancer progression. The present research for integrin β6 regulation mainly focuses on the post-transcription and translation related regulation mechanism and its role in tumorigenesis.

Research motivation

The mechanism of how the integrin β6 gene is regulated, and the promoter and transcription factors responsible for basic transcription of integrin β6 gene remain unknown.

Research objectives

This study aimed to clone and characterize the integrin β6 promoter.

Research methods

The region containing the integrin β6 promoter was predicted and luciferase reporter plasmids were constructed. The location of core promoter and binding site for transcription factors were identified by element deletion analysis.

Research results

We found that the regulatory elements for transcription of integrin β6 gene were located between -286 and-85 and contained binding sites for transcription factors such as STAT3 and Ets-1.

Research conclusions

We have identified the region of β6 core promoter and the binding sites for transcription factors such as Ets-1 and STAT3, which are important for integrin β6 promoter transcription activity.

Research perspectives

The findings of this study are important for investigating the mechanism of integrin β6 activation in cancer progression.