Collagen III combined with autologous adipose-derived mesenchymal stem cells accelerates burn wound healing in a rat model

Figure 1 Wound closure rates for the treatment group vs the control group. A: Wound size at different time points; B: The mean wound size of the two groups (n = 9) was analyzed by t-tests. ^bP < 0.01. ASCs: Adipose-derived stem cells.

Figure 2 Dermis layer assessment of recovered skin. A: Hematoxylin and eosin staining results of the dermal layer thickness; B: Statistical analysis of the average dermal layer thickness (n = 9, P = 0.0629); C: Masson staining results of the collagen deposition thickness; D: Statistical analysis of the average collagen deposition thickness (n = 9, P = 0.0413). ^aP < 0.05. ASCs: Adipose-derived stem cells.

Figure 3 Immunohistochemical staining against CD31. A: There were more micro-blood vessels in the collagen III group; B: Larger blood vessels were found in the treatment group. The orange arrows indicate some key areas of interest within the figure; C: The average number of micro blood vessels of the dermal layer was significantly different (n = 9, P < 0.0001). ^bP < 0.01. ASCs: Adipose-derived stem cells.

Figure 4 Green fluorescence detection of adipose-derived stem cells. A: Zs-Green adipose-derived stem cells infected with lentivirus grow well in culture; B: Positive control; C: Negative control; D: No Zs-Green labeled adipose-derived stem cells were found in either group. ASCs: Adipose-derived stem cells.