Zhou XL, Wu B, Xie ZJ, Li HD. Collagen III combined with autologous adipose-derived mesenchymal stem cells accelerates burn wound healing in a rat model. World J Stem Cells 2025; 17(5): 101898 [PMID: 40503366 DOI: 10.4252/wjsc.v17.i5.101898]
Corresponding Author of This Article
Hong-De Li, PhD, Hunan Provincial University Key Laboratory of the Fundamental and Clinical Research on Functional Nucleic Acid, College of Laboratory Medicine, Changsha Medical University, No. 1501 Leifeng Avenue, Changsha 410219, Hunan Province, China. azbyccx@163.com
Research Domain of This Article
Cell & Tissue Engineering
Article-Type of This Article
Basic Study
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
World J Stem Cells. May 26, 2025; 17(5): 101898 Published online May 26, 2025. doi: 10.4252/wjsc.v17.i5.101898
Collagen III combined with autologous adipose-derived mesenchymal stem cells accelerates burn wound healing in a rat model
Xiao-Long Zhou, Ben Wu, Zi-Jing Xie, Hong-De Li
Xiao-Long Zhou, Hunan Provincial Key Laboratory of the Traditional Chinese Medicine Agricultural Biogenomics, College of Pharmacy, Changsha Medical University, Changsha 410219, Hunan Province, China
Ben Wu, College of Pharmacy, Changsha Medical University, Changsha 410219, Hunan Province, China
Zi-Jing Xie, Hunan Provincial University Key Laboratory of the Regional Characteristic Traditional Chinese Medicine Resources and Ecological Agriculture, College of Pharmacy, Changsha Medical University, Changsha 410219, Hunan Province, China
Hong-De Li, Hunan Provincial University Key Laboratory of the Fundamental and Clinical Research on Functional Nucleic Acid, College of Laboratory Medicine, Changsha Medical University, Changsha 410219, Hunan Province, China
Author contributions: Zhou XL performed the experiments and wrote the draft of the manuscript; Wu B provided animal care and experimental assistance; Xie ZJ analyzed the data and revised the paper; Li HD designed and supervised the study. All the authors have read and approved the final manuscript.
Supported by the Natural Science Foundation of Hunan Province, China, No. 2021JJ40638; the Research Foundation of Education Bureau of Hunan Province, China, No. 22B0897; and General Funding Project of Hunan Provincial Health Commission, China, No. 202202055404.
Institutional animal care and use committee statement: All procedures involving animals were reviewed and approved by the Institutional Animal Care and Use Committee of Changsha Medical University (IACUC protocol number: 2020032).
Conflict-of-interest statement: The authors report no relevant conflicts of interest for this article.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Data sharing statement: All data can be supplied for reasonable requests.
Open Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Hong-De Li, PhD, Hunan Provincial University Key Laboratory of the Fundamental and Clinical Research on Functional Nucleic Acid, College of Laboratory Medicine, Changsha Medical University, No. 1501 Leifeng Avenue, Changsha 410219, Hunan Province, China. azbyccx@163.com
Received: September 30, 2024 Revised: December 31, 2024 Accepted: April 15, 2025 Published online: May 26, 2025 Processing time: 238 Days and 0.8 Hours
Abstract
BACKGROUND
Skin is the largest organ in the human body and plays crucial roles in human health. Efficient and rapid healing of burn wounds is of great significance. While stem cell therapies have offered potential methods to treat burn wounds, relatively few have had success in clinical practice.
AIM
To investigate the effect of the combined application of collagen III and adipose-derived stem cells (ASCs) on vascular regeneration in skin wound healing.
METHODS
Burn wounds were created in 18 adult male Sprague-Dawley rats, and they were randomly divided into two groups. In the treatment group, each rat was injected with 4 × 106 Zs-Green-labeled autologous ASCs suspended in collagen III. In the control group, each rat was injected with collagen III. Each rat received six injections. Images of the wounds were acquired every 3 days.
RESULTS
Multiple injections of autologous ASCs improved wound closure rate more efficiently compared to the control group. Moreover, autologous ASCs do not survive long-term during the skin wound healing process.
CONCLUSION
This study demonstrated that multiple injections of autologous ASCs combined with collagen III accelerated burn wound healing by increasing collagen deposition and improving angiogenesis.
Core Tip: This study demonstrates that multiple injections of autologous adipose-derived mesenchymal stem cells combined with collagen III accelerated burn wound healing by increasing collagen deposition and improving angiogenesis. Moreover, the autologous adipose-derived mesenchymal stem cells had no long-term survival in the skin wound healing process. This study provides insight into novel treatments of skin wound healing. Further detailed investigations are necessary to fully understand the therapeutic mechanism of this innovative strategy.
