Construction of IL-2 gene-modified human hepatocyte and its cultivation with microcarrier

Table 1 The clnongeneicity rate of L-02/IL-2, L-02/Neo and L-02 cells

No. of repeated wells	L-02/IL-2	L-02/Neo	L-02
1	124	148	150
2	135	144	157
3	133	154	144
4	144	141	153
5	128	166	162
6	123	152	156
Mean value	131.2 (13.1%)b	150.8 (15.1%)	153.7 (15.4%)

^bP < 0.01, vs L-02/Neo and L-02 cells

Table 2 Values of biochemical items and IL-2 in supernatant of L-02/IL-2 cells cultured with microcarrier

	Cultivation of day
	0	2	4	6	8	10
ALB(mg/L)
L-02	0	8.58 ± 0.30	25.01 ± 0.50	53.81 ± 1.64	39.13 ± 1.22	12.16 ± 0.68
L-02/IL-2	0	8.23 ± 0.39	26.14 ± 0.33	52.54 ± 1.28	40.42 ± 1.15	11.87 ± 0.51
UREA(mmol/L)
L-02	0.64 ± 0.03	0.90 ± 0.06	2.45 ± 0.14	5.35 ± 0.13	2.92 ± 0.11	1.15 ± 0.07
L-02/IL-2	0.60 ± 0.05	0.85 ± 0.03	2.57 ± 0.23	5.29 ± 0.17	2.93 ± 0.14	1.08 ± 0.09
AST(IU/L)
L-02	1.0 ± 0.58	3.3 ± 0.75	14.0 ± 0.58	17.6 ± 0.98	46.6 ± 1.62	70.3 ± 1.80
L-02/IL-2	1.0 ± 0.43	3.5 ± 0.93	15.1 ± 0.65	18.6 ± 0.72	47.0 ± 1.69	72.1 ± 2.04
LDH(IU/L)
L-02	15.9 ± 0.69	25.3 ± 0.95	63.0 ± 1.41	87.4 ± 2.07	522 ± 11.7	687 ± 13.4
L-02/IL-2	14.5 ± 0.62	25.0 ± 1.05	64.6 ± 1.57	88.7 ± 2.35	534 ± 14.6	694 ± 15.7
IL-2 (pg/L)
L-02	0	< 10	33.2 ± 2.6	78.3 ± 3.5	43.6 ± 3.1	21.7 ± 1.8
L-02/IL-2	0	6456 ± 373b	23765 ± 688b	52180 ± 1483b	38643 ± 1104b	4360 ± 587b

^bP < 0.001, vs L-02 cells.