Original Articles
Copyright ©The Author(s) 2001.
World J Gastroenterol. Jun 15, 2001; 7(3): 352-356
Published online Jun 15, 2001. doi: 10.3748/wjg.v7.i3.352
Table 1 Primers for K-ras and APC gene sequencing
GenesRegionCodonSize of PCRATSequence
K-rasexon11-5416356 °C5-GACTGAATATAAACTTGTGG
5-CTGTATCAAAGAAGTGTCCT
K-rasexon231-84161BP54 °C5-GACTGTGTTTCTCCCTTCT
5-GGCAAATACACAAAGAAAG
APC15-A1263-1393390BP54 °C5-GTGTAGAAGATACTCCAATA
5-GTGAACTGACAGAAGTACAT
APC15-B1338-1436295BP56 °C5-CAGGGTTCTAGTTTATCTTC
5-TTCTGCTTGGTGGCATGGTT
APC15-C1412-1515310BP56 °C5-GGAATGGTAAGTGGCATAAT
5-AAATGGCTCATCGAGGCTCA
APC15-D1496-1596300BP56 °C5-ACTCCAGATGGATTTTCTTG
5-GGCTGGCTTTTTTGCTTTAC
Table 2 APC gene mutation
DNARegionCodonMutationAmino acid exchange
N 3 CRCA1354TTT→TTAPhe→Leu
N17 CRCA1309GAA→TAAStop cocon
N14 CRCB1389TCT→TTTSer→Phe
N 5 CRCB1357GGA→AGAGly→Arg
N21 CRCB1365GGT→GGCnonsense
N26 CRCB1398AGT→ACTSer→Thr
N28 CRCC1465GTG→GCGVal→Ala
N33 CRCD1547GAA→TAAStop codon
Tubular villous adenomaA1301ins1 AStop codon
Villous adenomaA1309GAA→TAAStop codon
Villous adenomaC1490ins8 TTATTACAFrame shift
Tubular villous adenomaB1367CAG→CACGln→His
ACFA1309GAA→TAAStop codon