Cell division cyclin 25C knockdown inhibits hepatocellular carcinoma development by inducing endoplasmic reticulum stress

Table 1 Primers used in this study

Gene	Forward	Reverse
CDC25C	5′-CCTTGGATTCATCTGGACCTCTG-3′	5′-ATGTGCTACGCTTTGCAATCTTC-3′
GRP78	5′-GAAGAGCTGTGCTCGGACCT-3′	5′-CAGCAGCTTCTGCACCTTGG-3′
XBP-1	5′-CAGCAAGTGGTGGATTTGGAAG-3′	5′-TCTTAACTCCTGGTTCTCAACCACA-3′
CHOP	5′-GTCACACGCACATCCCAAA-3′	5′-GTTCTTCCTTGCTCTTCCTCCTC-3′
Caspase 9	5′-GGGACAGTGGCAGTAATGTCTTCA-3′	5′-AACTCAATGCCCAGTGTTCCAG-3′
Caspase 3	5′-TGAGCAGAGTCAAAGGCTGGAA-3′	5′-AGCTATAATGCATGCTATGGTGGTC-3′
GAPDH	5′-TGTGTCCGTCGTGGATCTGA-3′	5′-TTGCTGTTGAAGTCGCAGGAG-3′

CDC25C: Cell division cyclin 25C; GRP78: Glucose-regulated protein 78; XBP-1: X-box binding protein-1; CHOP: C/EBP homologous protein.

Table 2 Primary antibodies used in this study

Antibody	Species	Catalog	Company	Dilution
CDC25C	Rabbit	E302	Cambridge, MA	1:1000
GRP78	Rabbit	3177S	Cell Signaling Technology	1:500
XBP-1	Rabbit	AF5110	Affinity Biosciences	1:500
CHOP	Mouse	66741-1-lg	Proteintech	1:200
Caspase 9	Rabbit	abs145753	Absin Bioscience	1:2000
Caspase 3	Rabbit	ab184787	Abcam Cambridge, United Kingdom	1:500
GAPDH	Rabbit	FD0063	Fdbio	1:2000

CDC25C: Cell division cyclin 25C; GRP78: Glucose-regulated protein 78; XBP-1: X-box binding protein-1; CHOP: C/EBP homologous protein.