RhoC, vascular endothelial growth factor and microvascular density in esophageal squamous cell carcinoma

Table 1 Ras homolog C mRNA relative content, expression of Ras homolog C mRNA and protein and vascular endothelial growth factor protein in normal esophageal mucosa, adjacent atypical hyperplasia, and cancer tissues

Site	Cases (n)	RhoC mRNA relative content	χ2	P value	RhoC mRNA expression			χ2	P value	RhoC protein expression			χ2	P value	VEGF protein expression			χ2	P value
					-	+	Positive rate			-	+	Positive rate			-	+	Positive rate
Normal esophageal mucosa tissues	62	0.653 ± 0.069			49	13	21%			50	12	19.4%			44	18	29%
Adjacent atypical hyperplasia tissues	31	0.731 ± 0.065	99.629	0.000	21	10	32.3%	47.735	0.000	20	11	35.5%	54.25	0.000	14	17	54.8%	18.994	0.000
Cancer tissues	62	0.902 ± 0.119			12	50	80.6%			10	52	83.9%			20	42	67.7%

RhoC: Ras homolog C; VEGF: Vascular endothelial growth factor.

Table 2 Ras homolog C mRNA relative content, expression of Ras homolog C mRNA and protein and vascular endothelial growth factor protein in esophageal squamous cell carcinoma and the relationship with clinicopathologic parameters

Clinicopathologic parameters	Cases(n)	RhoC mRNA relative content	F(t, χ2)	P value	RhoC mRNA expression			χ2	P value	RhoC protein expression			χ2	P value	VEGF protein expression			χ2	P value
					-	+	Positive rate			-	+	Positive rate			-	+	Positive rate
Gender
Male	36	0.922 ± 0.123	0.586	0.118	7	29	80.6	0.000	0.983	6	30	83.3	0.018	0.892	11	25	69.4	0.004	0.950
Female	26	0.874 ± 0.109			5	21	80.8			4	22	84.6			9	17	65.4
Age (yr)
≥ 60	33	0.911 ± 0.108	0.619	0.538	6	27	81.8	0.062	0.803	7	26	78.8	1.348	0.246	12	21	63.6	0.217	0.642
< 60	29	0.892 ± 0.132			6	23	79.3			3	26	89.7			8	21	72.4
Histologic grading
I	15	0.795 ± 0.056			7	8	53.3			7	8	53.3			8	7	46.7
II	25	0.898 ± 0.086	22.535	0.000	3	22	88.0	9.520	0.009	2	23	92.0	13.744	0.001	7	18	72.0	4.014	0.134
III	22	0.979 ± 0.128			2	20	90.9			1	21	95.5			5	17	77.3
Invasion depth
Shallow group	7	0.751 ± 0.036			4	3	42.9			4	3	42.9			6	1	14.3
Deep group	55	0.924 ± 0.111	8.716	0.000	8	47	85.5	7.219	0.007	6	49	89.1	9.812	0.002	14	41	74.5	10.319	0.001
Lymphatic metastasis
Without	42	0.840 ± 0.074	9.042	0.000	11	31	73.8	3.898	0.048	10	32	76.2	5.678	0.017	18	24	57.1	6.693	0.010
With	20	1.032 ± 0.086			1	19	95.0			0	20	100.0			2	18	90.0

RhoC: Ras homolog C; VEGF: Vascular endothelial growth factor.

Table 3 Relationship among Ras homolog C protein, Ras homolog C mRNA, vascular endothelial growth factor protein and CD105 staining of measure microvascular density

RhoC protein expression	n	RhoC mRNA expression		r	P value	VEGFexpression		r	P value	MVDCD105(mean ± SD)	r	P value	VEGF protein expression	n	MVDCD105(mean ± SD)	t	P value
		+	-			+	-
+	52	46	6	0.451	0.000	40	12	0.448	0.000	41.99 ± 9.83	0.451	0.000	+	42	38.88 ± 0.041	5.147	0.027
-	10	4	6			2	8			31.07 ± 6.38			-	20	36.45 ± 0.036

MVD_CD105: CD105 staining of measure microvascular density; RhoC: Ras homolog C; VEGF: Vascular endothelial growth factor.