Observational Study
Copyright ©2014 Baishideng Publishing Group Inc.
World J Gastroenterol. Jul 14, 2014; 20(26): 8681-8690
Published online Jul 14, 2014. doi: 10.3748/wjg.v20.i26.8681
Table 1 Sample demographics (n = 128)
Liver diseaseGender
Age (yr)
MenWomen21-4040-6061-80
Normal donorsTrimmed normal livers3233410
End-stage liversHCC2224164
Peri-HCC2224164
HBV cirrhosis2162187
Severe cirrhosis112472
Alcoholic cirrhosis50050
Table 2 Primer sequence for real-time RT-PCR analysis
GeneGenBankForwardReverse
number
AhRNM_001621CTGCCTTTCCCACAAGATGTAGTTATCCTGGCCTCCGTTT
CARNM_005122CTTCTCTCCTGACCGACCTGAGGCCTAGCAACTTCGCATA
CYP1A2NM_000761GGACAGCACTTCCCTGAGAGGAGGCAGTCTCCACGAACTC
CYP2B6NM_000767TCCAGTCTCAGCTCCCAAGTCTGGCCAACATGTCCCTACT
CYP2C9NM_000771CCACATGCCCTACACAGATGTGCCCTTGGGAATGAGATAG
CYP2C19NM_000769CAACAACCCTCGGGACTTTAGTCTCTGTCCCAGCTCCAAG
CYP2D6NM_000106CAGAGATGGAGAAGGCCAAGAGAACAGGTCAGCCACCACT
CYP2E1NM_000773CCCAAAGGATATCGACCTCAAGGGTGTCCTCCACACACTC
CYP3A4NM_017460ACCGTGACCCAAAGTACTGGGTTTCTGGGTCCACTTCCAA
CYP3A5NM_000777GGAGATGTTCCCCATCATTGGCCCCAAAGATGTCTTTCAA
CYP3A7NM_000765GAAACACAGATCCCCCTGAAAGGGAAATCAGGCTCCACTT
CYP4A11NM_000778CACCACAACCCAAAAGTGTGATTGTTTCCCGATGCAGTTC
FXRNM_005123ATTTTGACGGAAATGGCAACAGACCCTTTCAGCAAAGCAA
GADPHNM_002046ACAGTCAGCCGCATCTTCTTACGACCAAATCCGTTGACTC
PPARαY07619ACGATTCGACTCAAGCTGGTGTTGTGTGACATCCCGACAG
PXRNM_005123GGAATGTTGGCTGAATGCTTCTGCATGCTGCTTCACATTT
Table 3 mRNA expression of Cytochrome P450 enzymes and corresponding nuclear receptors
Normal liver (n = 35)
End-stage livers (n = 93)
GeneExpression%-foldmean ± SEExpression%-foldmean ± SE
AhR5.52-27.14.9114.7 ± 0.742.71-12847.217.4 ± 1.52
CYP1A220.5-53726.2234 ± 94.60.78-175224136 ± 23.9a
CAR0.55-14.125.67.87 ± 0.500.21-29313957.73 ± 0.86
CYP2B61.31-10277.917.1 ± 2.740.81-12815827.2 ± 2.77a
CYP2C914.4-1544107786 ± 57.93.74-1486387418 ± 34.8a
CYP2C1949.6-125425.2594 ± 40.21.75-1379788256 ± 23.9a
CYP2D610.7-31629.5149 ± 12.24.31-930216156 ± 15.6
CYP2E1210-357717.21708 ± 11517.7-4373247990 ± 75.4a
PXR14.2-18913.341.4 ± 4.953.01-87.129.033.0 ± 1.81
CYP3A4171-13577.94798 ± 49.28.21-1966240498 ± 48.6a
CYP3A522.2-65930.0278 ± 26.99.92-97998.7203 ± 19.1a
CYP3A786.3-105412.2450 ± 36.71.51-21371415479 ± 47.7
PPARα13.7-57.74.2129.4 ± 1.892.61-16964.827.6 ± 2.43
CYP4A1115.1-47831.6198 ± 17.21.41-74052489.2 ± 11.1a