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World J Gastroenterol. Jul 14, 2014; 20(26): 8681-8690
Published online Jul 14, 2014. doi: 10.3748/wjg.v20.i26.8681
Published online Jul 14, 2014. doi: 10.3748/wjg.v20.i26.8681
Expression of P450 and nuclear receptors in normal and end-stage Chinese livers
Hong Chen, Zhong-Yang Shen, Wang Xu, Tie-Yan Fan, Jun Li, The Institute of Organ Transplantation, The General Hospital of Chinese People’s Armed Police Forces, Beijing 100039, China
Yuan-Fu Lu, Jie Liu, Department of Pharmacology, Kay Lab for Basic Pharmacology of Ministry of Education, Zunyi Medical College, Zunyi 563003, Guizhou Province, China
Ming-Liang Cheng, Department of Infectious Diseases, Guiyang Medical College Hospital, Guiyang 550004, Guizhou Province, China
Author contributions: Chen H, Shen ZY, Cheng ML and Liu J designed the experiments; Chen H, Xu W, Fan TY and Li J collected samples and performed the experiments; Lu YF performed RT-PCR analysis; Cheng ML contributed reagents/analytic tools; Chen H and Liu J analyzed the data and wrote the manuscript.
Supported by Grants from the Chinese 863 project, No. 2012AA022409; and Guizhou Science and Technology Foundation, No. 2009-70019
Correspondence to: Dr. Zhong-Yang Shen, The Institute of Organ Transplantation, The General Hospital of Chinese People’s Armed Police Forces, No. 69 Yong-Ding Road, Beijing 100039, China. zhongyangshen@gmail.com
Telephone: +86-10-57976839 Fax: +86-10-68242910
Received: January 9, 2014
Revised: February 19, 2014
Accepted: April 8, 2014
Published online: July 14, 2014
Revised: February 19, 2014
Accepted: April 8, 2014
Published online: July 14, 2014
Core Tip
Core tip: This study examined the expression of P450 enzyme (CYP) genes in end-stage liver diseases, including hepatocellular carcinoma (HCC), peri-HCC tissue, hepatitis B virus cirrhosis, alcoholic cirrhosis, and severe cirrhosis, and compared to normal donor livers by real-time RT-PCR analysis. The 93 end-stage livers had much higher inter-individual variations in gene expression than 35 normal livers. In general, the end-stage livers had decreased expression of CYP1A2, CYP2B6, CYP2C9, CYP2C19, CYP2D6, CYP2E1, CYP3A4, CYP3A5, CYP3A7 and CYP4A11, as well as their corresponding nuclear receptor AhR, CAR, PXR, and PPARα. HCC had dramatic decreases in CYP gene expression and nuclear receptors compared with peri-HCC tissues.