Expression and significance of cyclooxygenase-2 mRNA in benign and malignant ascites

Table 1 Primer sequences of cyclooxygensae-2 and glyceraldehyde-3-phosphate dehydrogenase genes

Primer		Primer sequence	Product size
COX-2	Forward primer	5’-CTTGGGTGTCAAAGGTAA-3’	581 bp
	Reverse primer	5’-AGGGACTTGAGGAGGGTA-3’
GAPDH	Forward primer	5’-GTGGGGCGCCAGGCACCA-3’	146 bp
	Reverse primer	5’-CTCCTATGTCACGCACATTC-3’

COX-2: Cyclooxygensae-2; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.

Table 2 mRNA expression of cyclooxygensae-2 n (%)

Group	COX-2 mRNA expression		χ2	P value
	Positive	Negative
In benign and malignant ascites1			4.051	0.044
Benign ascites	1 (6.7)	14 (93.3)
Malignant ascites	9 (42.9)	12 (57.1)
Among different disease types in benign group2				1.000
Cirrhosis with ascites	1 (6.7)	9 (60)
Tuberculous ascites	0 (0.0)	5 (33.3)
Among different disease types in malignant group3			1.614	0.806
Ovarian cancer	4 (19.0)	3 (14.3)
Colon cancer	2 (9.5)	3 (14.3)
Liver cancer	2 (9.5)	4 (19.0)
Gastric cancer	1 (4.8)	1 (4.8)
Bladder cancer	0 (0.0)	1 (4.8)

¹Comparison was made between groups using Yates’ continuity correction;

²Comparison was made between groups using Fisher's exact probability test;

³Comparison between groups utilized χ² test; P > 0.05 was considered not statistically significant. P < 0.05 was considered statistically significant. COX-2: Cyclooxygensae-2.