Cytokeratin 8 is increased in hepatitis C virus cells and its ectopic expression induces apoptosis of SMMC7721 cells

Table 1 Primers used for real-time polymerase chain reaction and high fidelity

Name	Forward primer (5’-3’)	Reverse primer (5’-3’)
CK8 (172 bp)	AGCTGGAGTCTCGCCTGGAA	TGTGCCTTGACCTCAGCAATG
GAPDH (138 bp)	GCACCGTCAAGGCTGAGAAC	TGGTGAAGACGCCAGTGGA
CK8 (1465 bp)	ATGTCGACATGTCCATCAGGGTGAC	TAGGATCCCTTGGGCAGGACGTC

CK8: Cytokeratin 8; PCR: Polymerase chain reaction; GAPDH: Glyceraldehydes-3-phosphate dehydrogenase.

Table 2 Detection of hepatitis C virus at RNA level in transfected cellular supernatant

	HCV RNA in supernatant of Huh-7-HCV cells	HCV RNA in supernatant of Huh-7.5-HCV cells
24 h	5.73 × 105	9.48 × 105
48 h	1.38 × 106	6.40 × 106
72 h	3.00 × 104	9.29 × 104
96 h	6.62 × 103	1.43 × 104

HCV: Hepatitis C virus.