Heme oxygenase-1 protects donor livers from ischemia/reperfusion injury: The role of Kupffer cells

Table 1 Primers used for RT-PCR

Gene	Primer sequence	Size of amplified DNA (bp)	Annealing temperature (°C)
HO-1	F 5’ TGGAAGAGGAGATAGAGCGA 3’	451	53
HO-1	R 5’ TGTTGAGCAGGAAGGCGGTC 3’	451
CD14	F 5’ GGACCCGATCTCAACACCT 3’	377	55
CD14	R 5’ CCAGCAGTATCCCGCAGT 3’	377
β-actin	F 5’ CGGGAAATCGTGCGTGAC 3’	443	55
β-actin	R 5’ TGGAAGGTGGACAGCGAGG 3’	443

RT-PCR: Reverse-transcriptase polymerase chain reaction; F: Forward; R: Reverse.