Effect of 2-amino-2-[2-(4-octylphenyl) ethyl] propane-1,3-diol hydrochloride (FTY 720) on immune liver injury in mice

Table 1 Effect of FTY 720 on serum ALT and AST activities in BCG+LPS -induced liver injury (mean±SD).

Group	Number of mice	sALT Karman’s units/dL	sAST Karman’s units/dL
Normal	10	19.65±11.87a	108.00±22.37a
BCG (3 mg/mouse) +LPS (7.5 mg/mouse)	10	234.24±143.17	254.71±101.45
BCG (3 mg/mouse) +LPS (7.5 mg/mouse)	10	168.05±87.19	220.31±54.96
+FTY 720 1 mg/kg BCG (3 mg/mouse)+LPS (7.5mg/mouse)+FTY 720 3 mg/kg	10	162.411±77.43	214.21±32.34
BCG (3 mg/mouse)+LPS (7.5 mg/mouse)+FTY 720 6 mg/kg	10	119.411±77.45a	183.00±46.29a

^aP<0.05 vs BCG (3 mg/mouse)+LPS (7.5 μg/mouse) model groups.

Table 2 Effect of FTY720 on serum ALT and AST activities in Con A-induced liver injury (mean±SD).

Group	Number of mice	sALT Karman’s units/dL	sAST Karman’s units/dL
Normal	10	52.79±4.43a	92.17±19.93a
Con A 15 mg/kg	10	104.73±44.83	120.34±28.25
Con A15 mg/kg+FTY 720 1 mg/kg	10	99.69±37.15	118.27±22.47
Con A15 mg/kg+FTY 720 3 mg/kg	10	79.99±51.79	110.63±26.26
Con A15 mg/kg+FTY 720 6 mg/kg	10	65.82±36.32a	93.18±17.36a

^aP<0.05 vs Con A model group.

Table 3 Alteration of serum cytokines after treatment with FTY 720 in Con A-induced liver injury (mean±SD).

Group	Number of mice	IFN-g (10-3 mg/L)	IL-4 (10-3 mg/L)
Normal	10	0.25±0.03a	0.13±0.00a
Con A 15 mg/kg	10	0.93±0.49	0.17±0.02
Con A15 mg/kg+FTY 720 1 mg/kg	10	0.36±0.17a	0.15±0.01a
Con A15 mg/kg+FTY 720 3 mg/kg	10	0.33±0.10a	0.14±0.00a
Con A15 mg/kg+FTY 720 6 mg/kg	10	0.29±0.07a	0.03±0.01a

^aP<0.05 vs Con A model group.

Table 4 Effect of FTY720 on spleen cell apoptosis.

Group	FTY 720 concentration %	Apoptotic cells
Normal		7.03
FTY 720	1.87 mg/L	7.55
FTY 720	3.75 mg/L	12.08
FTY 720	7.50 mg/L	25.46