Liver Cancer
Copyright ©The Author(s) 2003.
World J Gastroenterol. Apr 15, 2003; 9(4): 683-687
Published online Apr 15, 2003. doi: 10.3748/wjg.v9.i4.683
Figure 3
Figure 3 Northern blot analysis of 2 genes to confirm the Atlas human cancer cDNA expression array. Four paired cases were used to determine these genes expression patterns. Twenty µg RNA was analyzed on a 1.2% denaturing agrose gel and trans-ferred onto a nylon membrane. 32P-labeled cDNA probes for these genes were hybridized to the RNA-blotted membranes. After stringent washes, membranes were exposed to X-ray film overnight at -70 °C. The same membranes were rehybridized with human β-actin for an RNA loading control (N, adjacent normal liver tissue; C, human HCC tissue).