Liver Cancer
Copyright ©The Author(s) 2003. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Apr 15, 2003; 9(4): 683-687
Published online Apr 15, 2003. doi: 10.3748/wjg.v9.i4.683
Gene expression profiles of hepatoma cell line HLE
Lian-Xin Liu, Zhi-Hua Liu, Hong-Chi Jiang, Wei-Hui Zhang, Shu-Yi Qi, Jie Hu, Xiu-Qin Wang, Min Wu
Lian-Xin Liu, Hong-Chi Jiang, Wei-Hui Zhang, Department of Surgery, the First Clinical College, Harbin Medical University, Harbin 150001, Heilongjiang Province, China
Lian-Xin Liu, Zhi-Hua Liu, Xiu-Qin Wang, Min Wu, National Labortory of Molecular Oncology, Department of Cell Biology, Cancer Institute, Chinese Academy of Medical Science & Peking Union Medical College, Beijing 100021, China
Shu-Yi Qi, Jie Hu, Department of VIP, the First Clinical College, Harbin Medical University, Harbin 150001, Heilongjiang Province, China
Author contributions: All authors contributed equally to the work.
Supported by China Key Program on Basic Research, No.Z-19-01-01-02; Chinese Climbing Project, No.18; Youth Natural Scientific Foundation of Heilongjiang Province and Harbin, No.QC01C11
Correspondence to: Dr. Lian-Xin Liu, Department of Surgery, the First Clinical College of Harbin Medical University, No.23 Youzheng Street, Nangang District, Harbin 150001, China.
Telephone: +86-451-3668999 Fax: +86-451-3670428
Received: October 17, 2002
Revised: December 9, 2002
Accepted: December 16, 2002
Published online: April 15, 2003

AIM: To investigate the global gene expression of cancer related genes in hepatoma cell line HLE using Atlas Human Cancer Array membranes with 588 well-characterized human genes related with cancer and tumor biology.

METHODS: Hybridization of cDNA blotting membrane was performed with 32P-labeled cDNA probes synthesized from RNA isolated from Human hepatoma cell line HLE and non-cirrhotic normal liver which was liver transplantation donor. AtlasImage, a software specific to array, was used to analyze the result. The expression pattern of some genes identified by Atlas arrays hybridization was confirmed by reverse transcription polymerase chain reaction (RT-PCR) in 24 pairs of specimens and Northern blot of 4 pairs of specimens.

RESULTS: The differential expression of cell cycle/growth regulator in hepatocellular carcinoma (HCC) showed a stronger tendency toward cell proliferation with more than 1.5-fold up-regulation of Cyclin C, ERK5, ERK6, E2F-3, TFDP-2 and CK4. The anti-apoptotic factors such as Akt-1 were up-regulated, whereas the promotive genes of apoptosis such as ABL2 were down-regulated. Among oncogene/tumors suppressors, SKY was down-regulated. Some genes such as Integrin beta 8, Integrin beta 7, DNA-PK, CSPCP, byglycan, Tenacin and DNA Topo were up-regulated. A number of genes, including LAR, MEK1, eps15, TDGF1, ARHGDIA were down-regulated. In general, expression of the cancer progression genes was up-regulated, while expression of anti-cancer progression genes was down-regulated. These differentially expressed genes tested with RT-PCR were in consistent with cDNA array findings.

CONCLUSION: Investigation of these genes in HCC is helpful in disclosing molecular mechanism of pathogenesis and progression of HCC. For the first time few genes were discovered in HCC. Further study is required for the precise relationship between the altered genes and their correlation with the pathogenesis of HCC.

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