Molecular clone constructed by the fragment of chromosomal DNA from Helicobacter pylori

doi:10.3748/wjg.v4.iSuppl2.105

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Oct 15, 1998 (publication date) through Apr 18, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Oct 15, 1998; 4(Suppl2): 105-105
Published online Oct 15, 1998. doi: 10.3748/wjg.v4.iSuppl2.105

Molecular clone constructed by the fragment of chromosomal DNA from Helicobacter pylori

Xiu-Ying Pan, Wen-Yu Lin, Shen-Wu Wang, Guo-Dong Cao, Yong Liu

Xiu-Ying Pan, Wen-Yu Lin, Shen-Wu Wang, Guo-Dong Cao, Yong Liu, Department of People's Hospital, Beijing Medical University, Beijing 100044, China

Author contributions: All authors contributed equally to the work.

Correspondence to: Dr. Xiu-Ying Pan, Department of People's Hospital, Beijing Medical University, Beijing 100044, China

Received: June 28, 1998
Revised: August 7, 1998
Accepted: August 29, 1998
Published online: October 15, 1998

Abstract

AIM: Helicobacter pylori (Hp) was related to chronic gastritis and peptic ulcer. The fragment of chromosomal DNA from Hp amplified by PCR was cloned, which is significant on the basic research and clinical test for the positive control.

METHODS: Primer paris was designed from the fragment of Hp chromosome and the fragment was amplified by PCR, then the amplified product was inserted to pBluescript SK vector and transformed into E.coli DH5α. The constructed clone of Hp DNA was certified by PCR, restrict endo-enzy me digestion and DNA sequencing.

RESULTS: PCR product was showed to be 203 bp electronic band, consistent to that of Hp standard strain. Analysis of Hp-DNA sequence was similar to known Hp sequence.

CONCLUSION: The Hp DNA clone is used as the positive control in the Hp test.

Key Words: Helicobacter pylori, DNA, bacterial, Molecular cloning, Chromosome

Citation: Pan XY, Lin WY, Wang SW, Cao GD, Liu Y. Molecular clone constructed by the fragment of chromosomal DNA from Helicobacter pylori. World J Gastroenterol 1998; 4(Suppl2): 105-105
URL: https://www.wjgnet.com/1007-9327/full/v4/iSuppl2/105.htm
DOI: https://dx.doi.org/10.3748/wjg.v4.iSuppl2.105

Footnotes

E- Editor: Li RF