Published online Oct 15, 1998. doi: 10.3748/wjg.v4.iSuppl2.105
Revised: August 7, 1998
Accepted: August 29, 1998
Published online: October 15, 1998
AIM: Helicobacter pylori (Hp) was related to chronic gastritis and peptic ulcer. The fragment of chromosomal DNA from Hp amplified by PCR was cloned, which is significant on the basic research and clinical test for the positive control.
METHODS: Primer paris was designed from the fragment of Hp chromosome and the fragment was amplified by PCR, then the amplified product was inserted to pBluescript SK vector and transformed into E.coli DH5α. The constructed clone of Hp DNA was certified by PCR, restrict endo-enzy me digestion and DNA sequencing.
RESULTS: PCR product was showed to be 203 bp electronic band, consistent to that of Hp standard strain. Analysis of Hp-DNA sequence was similar to known Hp sequence.
CONCLUSION: The Hp DNA clone is used as the positive control in the Hp test.