Case Control Study
Copyright ©The Author(s) 2017. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Apr 28, 2017; 23(16): 2948-2956
Published online Apr 28, 2017. doi: 10.3748/wjg.v23.i16.2948
Recent upper gastrointestinal panendoscopy increases the risk of pyogenic liver abscess
Ming-Jen Tsai, Chin-Li Lu, Ying C Huang, Chung Hsien Liu, Wan-Ting Huang, Kai-Yuan Cheng, Solomon Chih-Cheng Chen
Ming-Jen Tsai, Ying C Huang, Chung Hsien Liu, Kai-Yuan Cheng, Department of Emergency Medicine, Ditmanson Medical Foundation Chia-Yi Christian Hospital, Chiayi 60002, Taiwan
Ming-Jen Tsai, Department of Sports Management, Chia Nan University of Pharmacy and Science, Tainan 71710, Taiwan
Chin-Li Lu, Department of Public Health, College of Medicine, National Cheng-Kung University, Tainan 70101, Taiwan
Ying C Huang, Department of Emergency Medicine, Medical Center and School of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan
Wan-Ting Huang, Department of Medical Research, Ditmanson Medical Foundation Chia-Yi Christian Hospital, Chiayi 60002, Taiwan
Solomon Chih-Cheng Chen, Heng Chun Christian Hospital, Pingtung County 946, Taiwan
Solomon Chih-Cheng Chen, Department of Pediatrics, School of Medicine, College of Medicine, Taipei Medical University, Taipei 110, Taiwan
Author contributions: Tsai MJ and Cheng KY contributed equally to this study and shared the first authorship; Tsai MJ, Cheng KY and Chen SCC conceptualized and designed the research; Chen SCC applied for the dataset from the National Health Research Institute; Lu CL and Huang WT collected and analyzed the data; all authors discussed the results; Tsai MJ and Cheng KY drafted the manuscript; Huang YC and Chen SCC critically reviewed the paper; all authors discussed the feasibility of the research and the results, and approved the final manuscript.
Institutional review board statement: The Institutional Review Board of Ditmanson Medical Foundation Chia-Yi Christian Hospital has approved this study (CYCH-IRB No. 104034).
Informed consent statement: No written informed consent is obtained since all the data have been de-identified before analysis.
Conflict-of-interest statement: The authors declare no conflicts of interest.
Data sharing statement: Dataset if available from the corresponding author at Solomon.ccc@gmail.com. No additional data are available.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Solomon Chih-Cheng Chen, MD, MS, PhD, Heng Chun Christian Hospital, No. 21, Hengxi Road, Hengchun Township, Pingtung County 946, Taiwan. solomon.ccc@gmail.com
Telephone: +886-928901217 Fax: +886-88880111
Received: January 5, 2017
Peer-review started: January 8, 2017
First decision: January 19, 2017
Revised: February 1, 2017
Accepted: March 30, 2017
Article in press: March 30, 2017
Published online: April 28, 2017

Abstract
AIM

To investigate the association between a recent gastrointestinal (GI) endoscopy and the subsequent risk of pyogenic liver abscess (PLA).

METHODS

We designed a nested case control study. Using the Taiwan National Health Insurance Research Database, 2135 patients with a first diagnosis of PLA were identified from 1998 to 2011. Another 10675 patients without PLA matched by age and sex were selected as reference controls. We identified and compared the possible risk factors for PLA and GI endoscopies performed before the index date (when PLA was diagnosed) between the two cohorts. Multivariate analysis was conducted to examine the risk of PLA within the 90 d after the GI endoscopies.

RESULTS

Patients with a history of diabetes [adjusted odds ratio (aOR) = 4.92, 95%CI: 1.78-13.61], end-stage renal disease (aOR = 3.98, 95%CI: 1.45-10.91), biliary tract infection (aOR = 2.68, 95%CI: 2.11-3.40), liver cirrhosis (aOR = 2.19, 95%CI: 1.39-3.46), GI malignancies (aOR = 5.68, 95%CI: 4.23-7.64), appendicitis (aOR = 3.16, 95%CI: 2.27-4.41), diverticulitis (aOR = 1.64, 95%CI: 1.01-2.64), and recent endoscopic retrograde cholangiopancreatography (aOR = 27.04, 95%CI: 11.65-62.72) were significantly associated with an increased risk of PLA. After adjusting for the above risk factors and the frequency of outpatient department visits and abdominal ultrasounds during 90 d before the index date, an upper GI panendoscopy (aOR = 2.75, 95%CI: 2.05-3.69) but not a lower GI endoscopy (aOR = 1.07, 95%CI: 0.62-1.86) was significantly associated with PLA.

CONCLUSION

An upper GI panendoscopy performed before 90 d may increase the risk of PLA.

Key Words: Appendicitis, Colonoscopy, Diverticulitis, Gastrointestinal endoscopy, Panendoscopy, Pyogenic liver abscess

Core tip: A pyogenic liver abscess (PLA) is a potential lethal disease with known pathogeneses, including biliary tract infection and portal venous bacterial spreading. Gastrointestinal (GI) endoscopies are common procedures that sometimes have complications of mucosa trauma, local infection, and bacteremia. The relationship between GI endoscopy and subsequent PLA has not yet been documented. This large nested case-control study has shown a significant association between a recent upper GI panendoscopy and increased risk of PLA, though a lower GI endoscopy and the invasive procedure itself of a GI endoscopy did not increase the risk of PLA. Furthermore, patients with diabetes mellitus, end-stage renal disease, liver cirrhosis, biliary tract infection, and GI malignancies could also have a higher risk of PLA. In summary, clinical physician should not ignore the risk of development of PLA after patients receiving an upper GI panendoscopy, especially in those with diabetes mellitus, end-stage renal disease, liver cirrhosis, biliary tract infection, and GI malignancies.



INTRODUCTION

A pyogenic liver abscess (PLA) is the most common type of visceral abscess and is a potentially life-threatening disease with distinct incidence rates worldwide[1-3]. In western countries, the annual PLA incidence rate is around 1-2.3 per 100000[2-4] with an overall mortality of around 10%[2,4,5]. In Taiwan, the annual incidence has increased steadily from 11.2 per 100000 in 1996 to 17.6 per 100000 in 2004[1].

The direct ascending spread of bacteria from the biliary tract or a hematologic spread of bacteria from organs of the portal systems due to gastrointestinal lesions with mucosa defects or a compromised mucosa barrier[5,6], is the well-known pathogenesis. Therefore, the documented risk factors for PLA include diabetes mellitus (DM)[1,7], end-stage renal disease (ESRD)[8,9], biliary tract infection (BTI)[10], liver cirrhosis[11,12], colorectal cancer[13-17], hepatobiliary tract cancer[18,19], and endoscopic retrograde cholangiopancreatography (ERCP)-related biliary tract procedures[20-23]. In addition, serial case reports have also demonstrated that acute appendicitis or diverticulitis may result in PLA through bacterial spreading from portal systems[24-29].

Gastrointestinal (GI) endoscopies are common procedures for the diagnosis and treatment of GI diseases. Although the risk of adverse events associated with these procedures is low, complications still occasionally happen under extensive usage. Common complications of GI endoscopy include perforation, hemorrhage, and infection. According to the literature, the mean rate of bacteremia after ERCP ranges from 6.4% to 18%[21]. These bacteremia episodes may evolve into clinical complications, including cholangitis, cholecystitis, PLA, pancreatic pseudocyst infection and even sepsis, at a rate ranging from 0.04% to 8.6%[20-23]. Hence, prophylaxis antibiotics are recommended for patients who receive ERCP with a bile duct obstruction and without adequate biliary drainage[20]. Transient bacteremia after a diagnostic upper GI (UGI) panendoscopy and colonoscopy has also been reported to occur at a mean rate of 4.4%[20,30,31]. Therefore, the development of PLA after a GI endoscopy through the hemorrhagic spread of bacteria is possible[20,24,31-33].

Until now, no large-scale study has been conducted to investigate the relationship between GI endoscopy and the subsequent risk of PLA. The aim of this study was to determine whether patients who had undergone a recent GI endoscopy had an increased risk of PLA compared to those who had not undergone GI endoscopy, based on a nationwide population-based database in Taiwan.

MATERIALS AND METHODS
Data source

This study was based on claims data from the Taiwan National Health Insurance (NHI) program, which was initiated in 1995 to provide affordable healthcare for all residents in Taiwan. There are currently more than 23 million enrollees in the program, representing over 99% of Taiwan’s entire population. For research purposes, claims data have been updated annually in the National Health Insurance Research Database (NHIRD) by the National Health Research Institute (NHRI). The NHRI released sets of sampling files, called the Longitudinal Health Insurance Database (LHID), for the year 2005 (LHID 2005). The NHRI randomly sampled 1000000 beneficiaries in 2005 from the entire population of NHI beneficiaries. All registrations and claims data for these 1000000 beneficiaries from 1996 to 2011 were included in the LHID 2005. The primary data source of this study was retrieved from the LHID 2005. In this cohort dataset, the original identification number of each patient was scrambled to protect patient privacy, thus patients’ informed consent was not needed. The study was approved by the Institutional Review Board of the Ditmanson Medical Foundation Chia-Yi Christian Hospital (CYCH-IRB 104034).

Study population and design

The present study was designed as a nested case-control study. We used LHID 2005 to identify patients with first-diagnosed PLA (International Classification of Disease, 9th Revision, Clinical Modification [ICD-9-CM], code 572.0) from either outpatient or inpatient medical records from 1998 to 2011. The diagnosis date of PLA served as the index date. For each patient with PLA, five matched controls were selected from the remaining patients in the database using an incidence density sampling method[34]. The controls were randomly selected from people who had a matched birth year and sex, and who had not been diagnosed with PLA from 1997 to the PLA occurrence date (index date) of their matched cases (Figure 1).

Figure 1
Figure 1 Flow chart for selecting cases and controls and identifying comorbidity and examinations.
Criteria and definitions

To evaluate the association between a recent digestive endoscopy and PLA, we reviewed inpatient and outpatient medical records of cases and controls in the 90 d period before the index date (Figure 1). Digestive endoscopy was determined by specific NHI order codes: UGI panendoscopy, 28016C; lower gastrointestinal (LGI) endoscopy; 28017C (colonoscopy); 28011C (rectoscopy); and 28013C (sigmoidoscopy). Endoscopy with or without invasive procedures, like biopsy, polypectomy, hemostasis and foreign body removal, were also determined by specific codes: 28030C (endoscopic biopsy); 47043B (endoscopic hemostasis); 47074C (panendoscopic polypectomy); 47083C (UGI foreign body removal); 28031C (colonoscopic or enteroscopic biopsy); 49014C (colonoscopic polypectomy); 49023C (rectoscopic hemostasis); 49025C (colonoscopy with removal of a foreign body); and 49026C (endoscopic hemostasis for colon bleeding). Because PLA had already been demonstrated as a complication of ERCP[20-23], we identified, controlled, and excluded ERCP-related procedures, including ERCP (33024B), endoscopic retrograde pancreas drainage (33033B), endoscopic retrograde biliary drainage (56020B), endoscopic papillotomy with stone extraction (56033B), endoscopic sphincterotomy (56031B), and endoscopic nasobiliary drainage (56021B), according to the specific codes. Moreover, to control other unidentified causes, usage dependence bias and confounding factors that may promote to a diagnosis of PLA, we also controlled for the frequency of outpatient department (OPD) visits and abdominal ultrasound examinations (19001C and 19009C) during the 90 d period before the index date in a multivariate analysis. Since the bureau of NHI regularly checked the claims dataset to ensure the validity of all procedure codes that patients received before reimbursement, we believe that the above endoscopy procedure records should be reliable. There was also a possibility that the patients who received endoscopic procedures were not recorded in the NHI database. For example, patients who received self-paid endoscopic procedures were not included. However, the NHI program covers near 100% of Taiwan’s entire population. The ratio of these self-paid endoscopic procedures was extremely low and can almost be neglected.

In addition to demographic variables, documented risk factors for PLA, including diabetes mellitus (ICD-9-CM code 250), BTI (ICD-9-CM codes 574.0-574.4, 575.0, 575.1, 575.10-575.12, and 576.1-576.3), liver cirrhosis (ICD-9-CM codes 571.2, 571.5, and 571.6), appendicitis (ICD-9-CM codes 540.0, 540.1, 541, and 542) and diverticulitis (ICD-9-CM codes 562.10-562.13), were also identified during the 15 mo (90 + 365 d) before the index date (Figure 1). Moreover, patients with ESRD or GI malignancies that were registered in the Critical Illness Database of NHIRD before the index date (not limited to the 15-month duration before the index date), were also identified (Figure 1). Malignancies of the GI tract included malignancies of colon (ICD-9-CM codes 153.0-153.9), rectosigmoid junction (154.0), rectum (154.1), anus (154.2, 154.3, and 154.8), liver (155.0 and 155.2), intrahepatic bile duct (155.1), gallbladder (156.0), extrahepatic bile ducts (156.1, 156.2, 156.8, and 156.9), stomach (151.0-151.9), small intestine (152.0-152.3, 152.8, and 152.9), and pancreas (157.0-157.4, 157.8, and 157.9).

Statistical analysis

Frequencies of demographic and clinical characteristics were described and compared between cases and controls using χ2 test. To evaluate the net effect of a recent UGI panendoscopy or LGI endoscopy on PLA risk, a multivariate analysis adjustment for the well documented or possible risk factors for PLA was conducted. The frequency of OPD visits and the frequency of abdominal ultrasound examinations were also adjusted to prevent a usage dependency bias for patients who had other risk and confounding factors of PLA which would lead to a higher frequency of OPD visits and ultrasound examinations. A conditional logistic regression model was performed to estimate the adjusted odds ratio (OR) and its 95% confidence interval (CI). Significance was set at P < 0.05. All statistical analyses were performed using SAS (version 9.3, SAS Institute Inc., Cary, NC, United States). In addition to the analysis of a recent 90 d GI endoscopy and PLA risk, we also conducted another sensitivity analysis of a recent 180 d and 1 year (365 d) GI endoscopy by the aforementioned methods to validate the association between GI endoscopy and PLA risk.

RESULTS
Demographics and comorbidities

During 1998 to 2011, 2135 PLA cases and 10675 matched controls were obtained from LHID 2005. The annual PLA incidence in Taiwan during this period was 15.25 cases per 100000. The mean age was 58.9 ± 16.5 year and 60% were males. PLA mostly happened in those aged 45-64 year (41.4%). The leading comorbidities of patients with PLA were BTI (11.7%), diabetes (9.7%), and liver cirrhosis (6.8%) (Table 1). Patients with PLA were more likely to have the following comorbidities than patients without PLA: diabetes (P < 0.001), ESRD (P < 0.001), BTI (P < 0.001), liver cirrhosis (P < 0.001), malignancies of the GI tract (P < 0.001), appendicitis (P < 0.001), and diverticulitis (P < 0.001) (Table 1).

Table 1 Demographic features and comorbidities of pyogenic liver abscess cases and controls n (%).
Demographic and clinical characteristicsCases(n = 2135)Controls(n = 10675)Total(n = 12810)P value
Age, yr
< 2031 (1.5)164 (1.5)1950.991
20-44391 (18.3)1952 (18.3)2343
45-64883 (41.4)4423 (41.4)5306
65-74454 (21.3)2229 (20.9)2683
75+376 (17.6)1907 (17.9)2283
mean ± SD58.8 ± 16.558.8 ± 16.558.8 ± 16.50.991
Gender
Female854 (40.0)4270 (40.0)51241.000
Male1281 (60.0)6405 (60.0)7686
Diabetes
With206 (9.7)253 (2.4)459< 0.001
Without1929 (90.4)10422 (97.6)12351
ESRD
With46 (2.2)73 (0.7)119< 0.001
Without2089 (97.9)10602 (99.3)12691
BTI
With249 (11.7)99 (0.9)348< 0.001
Without1886 (88.3)10576 (99.1)12462
Liver cirrhosis
With146 (6.8)97 (0.9)243< 0.001
Without1989 (93.2)10578 (99.1)12567
GI malignancy
With62 (2.9)80 (0.8)142< 0.001
Without2073 (97.1)10595 (99.3)12668
Appendicitis
With10 (0.47)9 (0.08)19< 0.001
Without2125 (99.53)10666 (99.92)12791
Diverticulitis
With12 (0.56)11 (0.1)23< 0.001
Without2123 (99.44)10664 (99.9)
Examinations and risk of pyogenic liver abscess

During the 90 d period before the index date, patients with PLA had a significantly higher rate of having undergone an UGI panendoscopy (P < 0.001), LGI endoscopy (P < 0.001), and ERCP-related procedures (P < 0.001) than patients without PLA. Fifty seven (33.1%) PLA cases occurred within the first 10 d after an endoscopic examination (Figure 2), with a median of 25.5 d (interquartile range, 7-53 d). Moreover, the frequency of OPD visits (P < 0.001) and frequency of abdominal ultrasound examinations (P < 0.001) during the 90 d period before the index date were also significantly higher in patients with PLA (Table 2).

Table 2 Outpatient visits and examination of pyogenic liver abscess cases and controls n (%).
Demographic and clinical characteristicsCases(n = 2135)Controls(n = 10675)Total(n = 12810)P value
Frequency of OPD visits
< 1119 (5.6)2781 (26.1)2900< 0.001
1-2243 (11.4)1989 (18.6)2232
3-7774 (36.3)3403 (31.9)4177
≥ 8999 (46.8)2502 (23.4)3501
Frequency of abdominal ultrasound
01692 (79.3)10329 (96.8)12021< 0.001
1358 (16.8)321 (3.0)679
≥ 285 (4.0)25 (0.2)110
Abdominal ultrasound
with443 (20.8)346 (3.2)789< 0.001
without1692 (79.3)10329 (96.8)12021
ERCP-related procedures
with107 (5.0)9 (0.1)116< 0.001
without2028 (95.0)10666 (99.9)12694
Upper GI panendoscopy
with172 (8.1)131 (1.2)303< 0.001
without1963 (91.9)10544 (98.8)12507
Lower GI endoscopy
with36 (1.7)53 (0.5)89< 0.001
without2099 (98.3)10622 (99.5)12721
Upper or lower GI endoscopy
with189 (8.9)171 (1.6)360< 0.001
without1946 (91.2)10504 (98.4)12450
Upper GI panendoscopy
upper GI only1150 (7.0)130 (1.2)280< 0.001
upper GI with ERCP-related procedures22 (1.0)1 (0.0)23
ERCP without upper GI85 (4.0)8 (0.1)93
None21878 (88.0)10536 (98.7)12414
Lower GI endoscopy
lower GI only134 (1.6)53 (0.5)87< 0.001
lower GI with ERCP-related procedures2 (0.1)0 (0.0)2
ERCP without lower GI105 (4.9)9 (0.1)114
None21994 (93.4)10613 (99.4)12607
Upper or lower GI endoscopy
upper or lower GI only1166 (7.8)170 (1.6)336< 0.001
upper or lower GI with ERCP-related procedures23 (1.1)1 (0.0)24
ERCP without upper or lower GI84 (3.9)8 (0.1)92
None21862 (87.2)10496 (98.3)12450
Figure 2
Figure 2 Frequency and duration of the diagnosis of pyogenic liver abscess after the first upper gastrointestinal panendoscopy examination. PLA: Pyogenic liver abscess; UGI: Upper gastrointestinal.

The net effect of a recent UGI panendoscopy and LGI endoscopy and PLA risk was evaluated by a multivariate analysis after adjusting for risk factors for PLA, recent ERCP-related procedures, and frequency of OPD visits and abdominal ultrasounds (Table 3). The results showed that patients who had undergone a recent UGI panendoscopy had significantly higher odds of having PLA than patients who had not undergone an UGI panendoscopy (OR = 2.75, 95%CI: 2.05-3.69, P < 0.001). The number needed to harm (NNH) for UGI panendoscopy in PLA was 15 patients (95%CI: 12-17). However, there was no significant difference between patients with or without a LGI endoscopy (OR = 1.07, 95%CI: 0.62-1.86, P = 0.803). Because a few cases who received an UGI panendoscopy or LGI endoscopy also received ERCP-related procedures during the preceding 90 d (Table 2), we also used another multivariate analysis model to calculate the OR of only UGI panendoscopy or LGI endoscopy (i.e., patients with concurrent ERCP-related procedures were excluded). The results still showed that an UGI panendoscopy was significantly associated with PLA (OR = 2.70, 95%CI: 2.01-3.63, P < 0.001; NNH = 17, 95%CI: 14-21) (Supplementary Table 1). Another sensitivity analysis conducted for a recent 180 d and 1 year GI endoscopy, and risk of PLA, had similar results. The OR for PLA risk was higher during the previous 90 d (OR = 2.75) than the 180 d (OR = 2.33, P < 0.001) and 1 year UGI panendoscopy (OR = 1.75, P < 0.001).

Table 3 Risk of pyogenic liver abscess related to comorbidities and examination n (%).
aOR95%CIP value
DiabetesWith vs without4.921.78-13.610.002
ESRDWith vs without3.981.45-10.910.007
BTIWith vs without2.682.11-3.40< 0.001
Liver cirrhosisWith vs without2.191.39-3.46< 0.001
GI malignancyWith vs without5.684.23-7.64< 0.001
AppendicitisWith vs without3.162.27-4.41< 0.001
DiverticulitisWith vs without1.641.01-2.640.044
Frequency of OPD visits1-2 vs < 12.652.10-3.34< 0.001
3-7 vs < 14.753.86-5.85< 0.001
8+ vs < 16.725.43-8.32< 0.001
Frequency of Ultrasound≥ 2 vs 03.262.69-3.95< 0.001
1 vs 05.693.35-9.66< 0.001
ERCP-related proceduresWith vs without27.0411.65-62.72< 0.001
UGI panendoscopyWith vs without2.752.05-3.69< 0.001
LGI EndoscopyWith vs without1.070.62-1.860.803
Comorbidities and risk of pyogenic liver abscess

Diabetes (OR = 4.92, 95%CI: 1.78-13.61, P = 0.002), ESRD (OR = 3.98, 95%CI: 1.45-10.91, P = 0.007), BTI (OR = 2.68, 95%CI: 2.11-3.40, P < 0.001), liver cirrhosis (OR = 2.19, 95%CI: 1.39-3.46, P < 0.001), history of GI tract malignancies (OR = 5.68, 95%CI: 4.23-7.64, P < 0.001), and ERCP-related procedures (OR = 27.04, 95%CI: 11.65-62.72, P < 0.001), which have been documented previously as PLA risk factors, were also significantly associated with PLA in this study. Additionally, patients with a history of appendicitis (OR = 3.16, 95%CI: 2.27-4.41, P < 0.001) or diverticulitis (OR = 1.64, 95%CI: 1.01-2.64, P = 0.044) also had significantly higher odds of having PLA than patients without appendicitis or diverticulitis, but the case numbers of these two illnesses were small (Table 3).

Invasiveness of endoscopy and risk of pyogenic liver abscess

We further analyzed the association between the risk of PLA and the invasiveness of UGI or LGI tract endoscopy. The results showed no significant association with PLA risk on whether the invasive procedures were performed or not in both an UGI panendoscopy (OR = 0.91, 95%CI: 0.47-1.77) and LGI endoscopy (OR = 0.97, 95%CI: 0.32-2.96) (Table 4).

Table 4 Risks of pyogenic liver abscess related to site and invasiveness of the digestive endoscopy performed n (%).
Cases (n = 2135)Controls (n = 10675)TotalaOR195%CIP value
Upper GI panendoscopy
Without invasive procedure2130 (6.09)98 (0.92)228Ref.
With invasive procedure42 (1.97)33 (0.31)750.910.47-1.770.787
None31963 (91.94)10544 (98.77)125070.350.26-0.49< 0.001
Lower GI endoscopy
Without invasive procedure224 (1.12)38 (0.36)62Ref.
With invasive procedure12 (0.56)15 (0.14)270.970.32-2.960.958
None32099 (98.31)10622 (99.5)127210.660.35-1.230.193
Upper or Lower GI endoscopy
Without invasive procedure2138 (6.46)124 (1.16)262Ref.
With invasive procedure51 (2.39)47 (0.44)980.870.49-1.540.624
None31946 (91.15)10504 (98.4)124500.400.29-0.54< 0.001
DISCUSSION

This large nested case-control study has shown a significant association between a recent UGI panendoscopy and increased risk of PLA (OR = 2.75, 95%CI: 2.05-3.69, NNH = 15). However, a LGI endoscopy and the invasive procedure itself of a GI endoscopy did not seem to increase the risk of PLA.

Bacterial translocation of GI microbial flora into the bloodstream may occur during an endoscopy due to mucosal injury. Although the risk of infection in remote tissues (i.e., infective endocarditis) caused by endoscopy-related transient bacteremia is extremely low, local infection may occur in which a typically sterile space or tissue is breached and contaminated by an endoscopic accessory[20,21]. Once the local infection progresses, it may develop into portal venous bacteremia and lead to PLA.

Previous studies have shown that bacteremia occurred mostly within 30 min after GI endoscopy[21]. In the present study, we found that 33.1% of PLA occurred within 10 d after an UGI panendoscopy. In addition, UGI panendoscopy in the previous 90 d had higher OR for PLA than the previous 180 d and 1 year panendoscopy. As the PLA occurred after UGI panendoscopy and the OR subsided with time, a causal relationship between them is very likely. Though the mean duration that PLA occurred after an UGI panendoscopy was 32 d (median 25.5 d), the shortest duration of finding the PLA after panendoscopy was only 1 d. Therefore, the possibility of a PLA coincidentally exists when receiving panendoscopy could not be ignored, because some patients with early PLA may also present upper GI symptoms which lead them to receive an UGI panendoscopy. The reasonable incubation time of developing PLA after panendoscopy is an interesting and important issue and deserves further study.

Our study also demonstrated that an UGI panendoscopy was significantly associated with a subsequent risk of PLA, but a LGI endoscopy was not. The distance between the liver and the examined organs may be the reason for this finding. For example, the colon is further away from the portal venous and lymphatic circulations to the liver than the esophagus, stomach, and duodenum, therefore a LGI endoscopy might have a lower probability of occurrence of PLA. Moreover, a complicated mesenteric lymphatic defense system in the LGI tract may also decrease the probability of portal venous bacteremia induced by a LGI endoscopy. In addition, high intraluminal air pressure due to air inflation during a duodenum examination in an UGI panendoscopy may also increase the additional risk of retrograde bacterial translocation from the biliary tract and increase the probability of PLA. All of the above factors may explain our findings that an UGI panendoscopy was significantly associated with a subsequent higher risk of PLA than a LGI endoscopy.

In theory, invasive procedures including biopsy, polypectomy, hemostasis, or foreign body removal will result in direct mucosal damage. Presumably, the risk of bacterial translocation into local tissue or circulation will increase due to the endogenous microbial flora gaining a portal of entry. Previous studies have shown that an interventional endoscopy may cause a higher incidence of bacteremia[21,35]. Hence, prophylaxis antibiotics were suggested for some high risk procedures, especially in patients with liver cirrhosis and acute GI bleeding or valvular heart disease[35,36]. However, in this study we found no significant difference in the risk of PLA between a GI endoscopy with and without invasive procedures. One explanation is that an UGI panendoscopy itself is invasive enough to result in an increased risk of PLA. Another possibility may be that some patients receiving invasive procedures were prescribed with prophylactic antibiotics or had concurrent antibiotics usage. Thus, it prevented the development of PLA, since the use of antibiotics has been recommended for invasive procedures with a high risk of bacteremia[20,21,37].

PLA secondary to inflammatory diseases of the GI tract, like acute appendicitis or diverticulitis, have been described in serial case reports through the mechanism of portal bacteremia[25-29], but scarcely studies have verified their association. Recently, one study demonstrated appendectomy correlates with increased risk of PLA[38]. In our study, we identified patients with a diagnosis of acute appendicitis or diverticulitis during the 15 mo before having PLA. Although the ratio and sample size of a history of acute appendicitis or diverticulitis in patients with PLA were small [0.47% (n = 10) and 0.56% (n = 12), respectively, Table 1], both were significantly associated with an increased risk of PLA (OR = 3.16, 95%CI: 2.27-4.41 in acute appendicitis; and OR = 1.64, 95%CI: 1.01-2.64 in diverticulitis; Table 3). This finding should remind us that PLA may develop after having appendicitis or diverticulitis.

Because the dataset of this study has covered over a decade of cases, we also take the change of endoscopy procedure into consideration. The most-related points are the infection controls for the procedures, which has meant the carrying of lower infectious complications and it has highlighted the high level and up-to-date GI endoscopy disinfection procedures in Taiwan[39,40]. The etiology for the development of PLA after GI endoscopy can be iatrogenic and the positive bacterial culture rate from these GI instruments could be high[40]. Hence, manual washing, automated endoscope washer reprocessing and adequate drying/storage after rinsing with regular surveillance culture were recently advised to diminish iatrogenic infectious complications[40].

The use of a representative, nationwide, population-based sample to investigate the risk factors of PLA increases the validity of our results. This large sample size granted us the statistical power to detect differences between the study groups. Nevertheless, there are several limitations in the present study. First, similar to other studies that used administrative data, unmeasured confounders may exist in our study. Second, the use of prophylactic antibiotics for patients who underwent invasive endoscopy was not considered in the analysis, which might confine the interpretation of the antibiotics on reducing the risk of PLA. Third, because the study was based on an administrate claims dataset, the diagnosis of PLA was defined by ICD-9-CM rather than detail clinical information, coding error is possible. Further validation may be needed. However, the NHI regularly checked the claims dataset to ensure the validity before reimbursement, we believe the ratio of coding error is extremely low[41]. In addition, the NHIRD has been extensively used in research, the same definition of PLA has been published in peer-reviewed journals previously[7-9,42]. Finally, a lack of microbiologic data limited the analysis of the association among causative pathogens, site of endoscopy, and PLA. Further study with a larger sample size that adjusts for the influence of concurrent antibiotic use may be needed to verify these findings.

In conclusion, a higher risk of PLA was found in patients who had recently undergone an UGI panendoscopy, especially within the first 10 d after panendoscopy. Clinical physician should not ignore the risk of development of PLA after patients receiving an UGI panendoscopy, especially in those with diabetes mellitus, ESRD, liver cirrhosis, BTI, and GI malignancies.

COMMENTS
Background

A pyogenic liver abscess (PLA) is a potential lethal disease with known pathogeneses, including biliary tract infection (BTI) and portal venous bacterial spreading. Gastrointestinal (GI) endoscopies are common procedures that sometimes have complications of mucosa trauma, local infection, and bacteremia. The relationship between GI endoscopy and subsequent PLA has not been documented.

Research frontiers

This study investigates the association between a recent GI endoscopy and the subsequent risk of PLA.

Innovations and breakthroughs

A higher risk of PLA was found in patients who had recently undergone an upper GI panendoscopy, especially within the first 10 d after panendoscopy. Furthermore, patients with diabetes mellitus, end-stage renal disease, liver cirrhosis, BTI, and GI malignancies also could have a much higher risk of PLA.

Applications

These findings remind clinical physician that PLA may occur in those high-risk patients when they undergo an upper GI panendoscopy.

Peer-review

This manuscript provides the updated evidence to the readers. The topic is an important one and deserves a practical value.

Footnotes

Manuscript source: Unsolicited manuscript

Specialty type: Gastroenterology and hepatology

Country of origin: Taiwan

Peer-review report classification

Grade A (Excellent): A

Grade B (Very good): B, B

Grade C (Good): C, C

Grade D (Fair): 0

Grade E (Poor): 0

P- Reviewer: Cerwenka HR, Joseph Lo Z, Liao KF, Lee HC, Poovorawan K S- Editor: Gong ZM L- Editor: A E- Editor: Wang CH

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