High persistence rate of hepatitis B virus in a hydrodynamic injection-based transfection model in C3H/HeN mice

Figure 3 Longer expression of hepatitis B core antigen and hepatitis B surface antigen in the liver of C3H/HeN mice than C57BL/6 mice. C3H/HeN or C57BL/6 mice were hydrodynamically injected with 10 μg of pAAV/HBV1.2 plasmid, and the livers of C3H/HeN or C57BL/6 mice were collected at 5, 8 and 46 wk after HI. A: Detection of HBcAg expression in C3H/HeN or C57BL/6 mouse liver at 5, 8 and 46 wk after HI by immunochemistry; B: Detection of HBsAg expression in C3H/HeN or C57BL/6 mouse liver at 5, 8 and 46 wk after HI by chemistry. Images of specific antibody stained slides were observed under a microscope at magnification of x 400. Experiments were repeated twice with a similar pattern. HBcAg: Hepatitis B core antigen; HBsAg: Hepatitis B surface antigen; HI: Hydrodynamic injection.