Basic Study
Copyright ©The Author(s) 2015. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Mar 28, 2015; 21(12): 3527-3536
Published online Mar 28, 2015. doi: 10.3748/wjg.v21.i12.3527
High persistence rate of hepatitis B virus in a hydrodynamic injection-based transfection model in C3H/HeN mice
Xiu-Hua Peng, Xiao-Nan Ren, Li-Xiang Chen, Bi-Sheng Shi, Chun-Hua Xu, Zhong Fang, Xue Liu, Jie-Liang Chen, Xiao-Nan Zhang, Yun-Wen Hu, Xiao-Hui Zhou
Xiu-Hua Peng, Xiao-Nan Ren, Li-Xiang Chen, Bi-Sheng Shi, Chun-Hua Xu, Zhong Fang, Xue Liu, Jie-Liang Chen, Xiao-Nan Zhang, Yun-Wen Hu, Xiao-Hui Zhou, Shanghai Public Health Clinical Center, Key Laboratory of Medical Molecular Virology, Ministry of Education and Health, Fudan University, Shanghai 201508, China
Author contributions: Zhou XH conceived and designed the study; Peng XH, Ren XN, Chen LX, Shi BS, Xu CH, Fang Z, Liu X, Chen JL and Zhang XN performed the experiments; Peng XH, Ren XN, Chen LX and Zhou XH analyzed the data; Zhou XH, Ren XN, Chen LX, Chen JL, Zhang XN and Hu YW wrote and revised the paper.
Supported by Shanghai Municipal Fund for Science and Technology Development, No. 13140902200 and No. 12140900300; National Key Basic Research Development Program (973 Program), No. 2012CB519005.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Xiao-Hui Zhou, MD, PhD, Associate Professor, Shanghai Public Health Clinical Center, Key Laboratory of Medical Molecular Virology, Ministry of Education and Health, Fudan University, 2901 Caolang Road, Jin Shan District, Shanghai 201508, China. zhouxiaohui@shaphc.org
Telephone: +86-21-37990333 Fax: +86-21-37990333
Received: July 21, 2014
Peer-review started: July 21, 2014
First decision: August 15, 2014
Revised: September 15, 2014
Accepted: October 14, 2014
Article in press: October 15, 2014
Published online: March 28, 2015
Core Tip

Core tip: In the classic hepatitis B virus (HBV) hydrodynamic injection (HI) model using C57BL/6 mice, only about 30% of the injected mice carried HBV for more than 12 wk. Here we injected the pAAV-HBV1.2 plasmid into C3H/HeN mice and observed that the hepatitis B surface antigen, hepatitis B e antigen and viral DNA persisted even up to 46 wk in about 90% of the hydrodynamically injected mice. Applying interferon-α or entecavir in this HI model decreased HBV DNA in vivo. Hence, C3H/HeN is a suitable mouse strain for the persistent HBV HI model, which might be useful for chronic hepatitis B research and therapeutic drug development.