High persistence rate of hepatitis B virus in a hydrodynamic injection-based transfection model in C3H/HeN mice

Figure 1 Hepatitis B surface antigen, hepatitis B e antigen and hepatitis B virus DNA levels in serum of C3H/HeN and C57BL/6 mice after hydrodynamic injection. pAAV/HBV1.2 DNA was injected hydrodynamically into the tail vein of 5-6 wk male C3H/HeN and C57BL/6 mice. After injection, the mice were regularly bled to monitor the serum levels of HBsAg, HBeAg and HBV DNA. A: Titer of serum HBsAg in C3H/HeN or C57BL/6 mice after HI at different time points; B: Positive rates of serum HBsAg in C3H/HeN (n = 25) or C57BL/6 (n = 20) mice at different time points after HI; C: Titer of serum HBeAg in C3H/HeN or C57BL/6 mice after HI at different time points; D: Serum HBV DNA level was determined at different time points. HBsAg: Hepatitis B surface antigen; HBeAg: Hepatitis B e antigen; HBV: Hepatitis B virus; HI: Hydrodynamic injection.