Gene expression profiles of hepatoma cell line HLE

Figure 1 Parallel analyses of gene expression profiles in hu-man hepatoma cell line HLE and normal liver tissues. Atlas human cancer cDNA expression array (Clontech, USA) was hybridized with ³²P-labeled cDNA probes in normal liver tis-sues (A) and human hepatoma cell line HLE (B).

Figure 2 Partial semi-quantitative RT-PCR for 2 genes in 24 paired tissues. A total of 10 µl RT-PCR products were electro-phoresed on 2% agarose gel containing ethidium bromide. GAPDH was used as an internal control. (RT-PCR, reverse tran-scription polymerase chain reaction; N, adjacent normal liver tissue; C, human hepatocellular carcinoma tissue; GAPDH, glyceraldehyde-s-phosphate dehydrogenase; M, pUC Mix Maker).

Figure 3 Northern blot analysis of 2 genes to confirm the Atlas human cancer cDNA expression array. Four paired cases were used to determine these genes expression patterns. Twenty µg RNA was analyzed on a 1.2% denaturing agrose gel and trans-ferred onto a nylon membrane. ³²P-labeled cDNA probes for these genes were hybridized to the RNA-blotted membranes. After stringent washes, membranes were exposed to X-ray film overnight at -70 °C. The same membranes were rehybridized with human β-actin for an RNA loading control (N, adjacent normal liver tissue; C, human HCC tissue).