Mutation and methylation of hMLH1 in gastric carcinomas with microsatellite instability

Figure 1 MSI in gastric cancer using 5 microsatellite loci (BAT-25, BAT-26, BAT-40, D2S123, and D5S346). Arrows indicate variant conformers. N: normal DNA pattern; T: tumor speci-mens containing variant conformers representing MSI.

Figure 2 Detection of mismatching repair gene hMLH1 muta-tion in gastric cancer by two-dimentional DNA electrophoresis. Four-band pattern was observed at exon 8.

Figure 3 Methylation of hMLH1 in gastric cancer. A: No me-thylation was found in normal mucosa; B: Methylation of hMLH1 in MSI-H gastric cancer. Cases 1, 3 showed both hypermethylation and unmethylation; C: Methylation of hMLH1 in MSI-L gastric cancer. Case 3 showed both hypermethylation and unmethylation; U: Unmethylation; M: methylation.