Viral Hepatitis
Copyright ©The Author(s) 2003.
World J Gastroenterol. Oct 15, 2003; 9(10): 2226-2231
Published online Oct 15, 2003. doi: 10.3748/wjg.v9.i10.2226
Figure 1
Open in New Tab Full Size Figure Download Figure
Figure 1 PCR products of HCV structural protein genes. Lane1: DNA molecular weight marker (DL-2000), lane2: 5’NCR-CE1E2, lane3: CE1E2.
Figure 2
Open in New Tab Full Size Figure Download Figure
Figure 2 Confirmation of recombinant baculovirus donor plasmids. Lane1: PCR of pFB1-CS, lane 2: PCR of pFB1-CE1E2, lane 3: pFB1-CS digested by XhoI/Hind III, lane 4: pFB1-CE1E2 digested by XhoI/Hind III, lane 5: pFastBacI digested by BamHI/Hind III, lane 6: DL-15000 marker.
Figure 3
Open in New Tab Full Size Figure Download Figure
Figure 3 Confirmation of recombinant bacmids by PCR. Lane 1: bacmid/0 control, lane 2: bacmid/CS, lane 3: bacimd/CE1E2, lane 4: DL-15000 marker.
Figure 4
Open in New Tab Full Size Figure Download Figure
Figure 4 Baculoviruses in nuclei of Sf9 cells transfected with recombinant bacmid DNA (× 25000).
Figure 5
Open in New Tab Full Size Figure Download Figure
Figure 5 Confirmation of recombinant baculoviruses by PCR. Lane 1: BV/Bac control, lane 2: reBV/CS, lane 3: reBV/CE1E2, lane 4: DL-2000 marker.
Figure 6
Open in New Tab Full Size Figure Download Figure
Figure 6 Immunofluorescence analysis of expression of HCV structural proteins in Sf9 cells. Sf9 cells infected with A: reBV/CS, B: reBV/CE1E2, C: control BV/Bac.
Figure 7
Open in New Tab Full Size Figure Download Figure
Figure 7 Identification of HCV structural proteins expressed in Sf9 cells by SDS-PAGE. Lanes 1-2: cells infected with reBV/CS and reBV/CE1E2 respectively, lane 3: control cells infected with BV/Bac, lane 4: molecular weight marker.
Figure 8
Open in New Tab Full Size Figure Download Figure
Figure 8 Immunoblotting of anti-E2 antibody immunoprecipi-tated proteins with anti-E2 mAb (A), anti-C mAb (B) and anti-HCV serum from HCV-infected patients (C). Lane 1: control BV/Bac, lane 2: reBV/CE1E2, lane 3: reBV/CS.
Figure 9
Open in New Tab Full Size Figure Download Figure
Figure 9 Electron micrographs of baculovirus-infected Sf9 cells (× 25000). Sf9 cells infected with A: reBV/CS, B: reBV/CE1E2, C: control BV/Bac.
Figure 10
Open in New Tab Full Size Figure Download Figure
Figure 10 VLP band (indicated by arrow) in sucrose gradient centrifugation.
Figure 11
Open in New Tab Full Size Figure Download Figure
Figure 11 Electron microscopy and immune aggregation elec-tron microscopy analysis of VLPs (negative staining). A and B (× 100000): VLPs isolated from Sf9 cells infected with reBV/CS and reBV/CE1E2, respectively. C and D (× 150000): Immune aggregation of VLPs from A and B with anti-HCV serum, respectively.