Basic Study
Copyright ©The Author(s) 2018.
World J Gastroenterol. Feb 14, 2018; 24(6): 706-715
Published online Feb 14, 2018. doi: 10.3748/wjg.v24.i6.706
Figure 1
Open in New Tab Full Size Figure Download Figure
Figure 1 Administration of rVIPa inhibited the pathogenic effects and body weight loss caused by TNBS. A: The experimental scheme of induction of colitis and administration of rVIPa; B: Body weight of rats between days 1-10. rVIPa: Recombinant expressed vasoactive intestinal peptide analogue; TNBS: Trinitrobenzene sulfonic.
Figure 2
Open in New Tab Full Size Figure Download Figure
Figure 2 Histological observation and evaluation of colon tissue. A: Visual inspection of TNBS-induced colitis of colon tissue in rats and effect of rVIPa with different concentrations; B: Histologic score; C: Colon length. Data represent mean ± SEM (n = 8). aP < 0.05, eP < 0.001 vs control rats; cP < 0.05, dP < 0.01, fP < 0.001 vs TNBS-induced model rats.
Figure 3
Open in New Tab Full Size Figure Download Figure
Figure 3 Myeloperoxidase and total superoxide dismutase activities in serum and colon tissue. Data represent mean ± SEM (n = 8). bP < 0.01, eP < 0.001 vs control rats; dP < 0.01, fP < 0.001 vs TNBS-induced model rats. MPO: Myeloperoxidase; T-SOD: Total superoxide dismutase; rVIPa: Recombinant expressed vasoactive intestinal peptide analogue; TNBS: Trinitrobenzene sulfonic.
Figure 4
Open in New Tab Full Size Figure Download Figure
Figure 4 TNF-α, IL-10, and endotoxin level in serum and colon tissue. Data represent mean ± SEM (n = 8). bP < 0.01, eP < 0.001 vs Control rats; cP < 0.05, dP < 0.01, and fP < 0.001 vs TNBS-induced model rats. TNF-α: Tumor necrosis factor; IL-10: Interleukin-10; rVIPa: Recombinant expressed vasoactive intestinal peptide analogue; TNBS: Trinitrobenzene sulfonic.
Figure 5
Open in New Tab Full Size Figure Download Figure
Figure 5 Protein expression level of occludin and ZO-1 in colon. Data represent mean ± SEM (n = 8). bP < 0.01 vs control rats; cP < 0.05 vs TNBS-induced model rats. rVIPa: Recombinant expressed vasoactive intestinal peptide analogue; TNBS: Trinitrobenzene sulfonic.
Figure 6
Open in New Tab Full Size Figure Download Figure
Figure 6 Effects of rVIPa with different concentration on TLR4/NF-κB signaling proteins expression level in colon tissue. Data represent mean ± SEM (n = 8). bP < 0.01 vs control rats; cP < 0.05 vs TNBS-induced model rats. rVIPa: Recombinant expressed vasoactive intestinal peptide analogue; TLR4: Toll-like receptor 4; NF-κB: Nuclear factor of kappa B; rVIPa: Recombinant expressed vasoactive intestinal peptide analogue; TNBS: Trinitrobenzene sulfonic.