Basic Study
Copyright ©The Author(s) 2018. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Feb 14, 2018; 24(6): 706-715
Published online Feb 14, 2018. doi: 10.3748/wjg.v24.i6.706
Recombinant expressed vasoactive intestinal peptide analogue ameliorates TNBS-induced colitis in rats
Chun-Lan Xu, Yu Guo, Lei Qiao, Li Ma, Yi-Yi Cheng
Chun-Lan Xu, Yu Guo, Lei Qiao, Li Ma, Yi-Yi Cheng, The Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi’an 710072, Shaanxi Province, China
Author contributions: Xu CL conceived and designed the experiments; Guo Y, Qiao L and Ma L performed the experiments; Cheng YY analyzed the data; Roman A contributed reagents/materials/analysis tools; Xu CL wrote the manuscript.
Supported by the National Natural Science Foundation of China, No. 31672435; the Graduate Starting Seed Fund of Northwestern Polytechnical University, No. Z2017236; and the National College Students Innovation, Experiment Program, No. 201610699266.
Institutional review board statement: The study was reviewed and approved by the Northwestern Polytechnical University Institutional Review Board.
Institutional animal care and use committee statement: All procedures involving animals were reviewed and approved by the Institutional Animal Care and Use Committee of the Northwestern Polytechnical University (IACUC protocol number: No.2016014).
Conflict-of-interest statement: No potential conflicts of interest.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See:
Correspondence to: Chun-Lan Xu, PhD, Associate Professor, The Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, 127 Youyixi Road, Xi’an 710072, Shaanxi Province, China.
Telephone: +86-29-88460543 Fax: +86-29-88460332
Received: December 1, 2017
Peer-review started: December 1, 2017
First decision: December 13, 2017
Revised: December 19, 2017
Accepted: December 27, 2017
Article in press: December 27, 2017
Published online: February 14, 2018
Core Tip

Core tip: Vasoactive intestinal peptide (VIP) is a neuropeptide with potent anti-inflammatory activities. Recombinant VIP analogue (rVIPa with amino acid sequence “HSKAVFTKNYTRLRKQMAVKKYLNSILN”) with higher antimicrobial activity and stability than natural peptide was produced by an effective and low-cost production method. The current study first indicated that rVIPa could alleviated TNBS-induced colitis via TLR4/NF-κB-mediated signaling pathway. In summary, these results suggest a protective role and anti-inflammatory effect of rVIPa in inflammatory bowel disease and indicate that rVIPa has therapeutic potential for intestinal inflammatory disorders. The study contributes to identify and produce novel anti-inflammatory agents from human innate host defense mechanisms in the process of biological evolution.